Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.159 extracted from

  • Baron, S.F.; Franklund, C.V.; Hylemon, P.B.
    Cloning, sequencing, and expression of the gene coding for bile acid 7alpha-hydroxysteroid dehydrogenase from Eubacterium sp. strain VPI 12708 (1991), J. Bacteriol., 173, 4558-4569.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli DH5alpha, amino acid sequence analysis and comparison with other dehydrogenases Eubacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
wild type and recombinant proteins, non-denaturing PAGE Eubacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
bile acids + NAD(P)+ Eubacterium sp.
-
?
-
r
bile acids + NAD(P)+ Eubacterium sp. VPI 12708
-
?
-
r
additional information Eubacterium sp. cholic acid inducible ?
-
?
additional information Eubacterium sp. VPI 12708 cholic acid inducible ?
-
?

Organism

Organism UniProt Comment Textmining
Eubacterium sp.
-
-
-
Eubacterium sp. VPI 12708
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and recombinant from Escherichia coli Eubacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
332
-
NADP+, recombinant enzyme from Escherichia coli Eubacterium sp.
374
-
NADP+ Eubacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bile acids + NAD(P)+
-
Eubacterium sp. ?
-
r
bile acids + NAD(P)+
-
Eubacterium sp. VPI 12708 ?
-
r
cholic acid + NAD(P)+ inducible by cholic acid Eubacterium sp. 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH
-
r
cholic acid + NAD(P)+ inducible by cholic acid Eubacterium sp. VPI 12708 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH
-
r
glycochenodeoxycholic acid + NAD(P)+
-
Eubacterium sp. N-[3alpha-hydroxy-7,24-dioxocholan-24-yl]glycine + NAD(P)H
-
r
glycochenodeoxycholic acid + NAD(P)+
-
Eubacterium sp. VPI 12708 N-[3alpha-hydroxy-7,24-dioxocholan-24-yl]glycine + NAD(P)H
-
r
additional information cholic acid inducible Eubacterium sp. ?
-
?
additional information cholic acid inducible Eubacterium sp. VPI 12708 ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 32000-33000 Eubacterium sp.

Cofactor

Cofactor Comment Organism Structure
NAD+ nearly no activity with Eubacterium sp.
NADP+
-
Eubacterium sp.