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Literature summary for 1.1.1.159 extracted from

  • Zhu, D.; Stearns, J.E.; Ramirez, M.; Hua, L.
    Enzymatic enantioselective reduction of alpha-ketoesters by a thermostable 7alpha-hydroxysteroid dehydrogenase from Bacteroides fragilis (2006), Tetrahedron, 62, 4535-4539.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Bacteroides fragilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7-keto-lithocholic acid + NADH Bacteroides fragilis in the gastrointestinal tract of host organisms cheno-deoxycholic acid + NAD+
-
?

Organism

Organism UniProt Comment Textmining
Bacteroides fragilis
-
strain ATCC 25285
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Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli by heat treatment at 55-60┬░C, polyethyleneimine treatment, ammonium sulfate fractionation, and gel filtration, followed by lyophilization Bacteroides fragilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.32
-
purified recombinant enzyme, substrate methyl benzoylformate Bacteroides fragilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-keto-lithocholic acid + NADH
-
Bacteroides fragilis cheno-deoxycholic acid + NAD+
-
?
7-keto-lithocholic acid + NADH in the gastrointestinal tract of host organisms Bacteroides fragilis cheno-deoxycholic acid + NAD+
-
?
ethyl (R)-2-hydroxy-2-(3,5-difluorophenyl)acetate + NAD+
-
Bacteroides fragilis ethyl 2-oxo-2-(3,5-difluorophenyl)acetate + NADH
-
?
ethyl (R)-2-hydroxy-3,3-dimethylbutyrate + NAD+
-
Bacteroides fragilis ethyl 2-oxo-3,3-dimethylbutyrate + NADH
-
?
ethyl (R)-2-hydroxy-3-methylbutyrate + NAD+
-
Bacteroides fragilis ethyl 2-oxo-3-methylbutyrate + NADH
-
?
ethyl 2-hydroxy-2-(3,4-dichlorophenyl)acetate + NAD+
-
Bacteroides fragilis ethyl 2-oxo-2-(3,4-dichlorophenyl)acetate + NADH
-
?
ethyl 2-hydroxy-2-(4-bromophenyl)acetate + NAD+
-
Bacteroides fragilis ethyl 2-oxo-2-(4-bromophenyl)acetate + NADH
-
?
ethyl 2-hydroxy-2-(4-chlorophenyl)acetate + NAD+
-
Bacteroides fragilis ethyl 2-oxo-2-(4-chlorophenyl)acetate + NADH
-
?
ethyl 2-hydroxy-2-(4-cyanophenyl)acetate + NAD+ high activity Bacteroides fragilis ethyl 2-oxo-2-(4-cyanophenyl)acetate + NADH
-
?
ethyl 2-hydroxy-2-(4-fluorophenyl)acetate + NAD+
-
Bacteroides fragilis ethyl 2-oxo-2-(4-fluorophenyl)acetate + NADH
-
?
ethyl 2-hydroxy-2-(4-methylphenyl)acetate + NAD+
-
Bacteroides fragilis ethyl 2-oxo-2-(4-methylphenyl)acetate + NADH
-
?
ethyl 2-hydroxy-2-cyclohexylacetate + NAD+ best substrate Bacteroides fragilis ethyl 2-oxo-2-cyclohexylacetate + NADH
-
?
ethyl 2-hydroxy-2-phenylacetate + NAD+
-
Bacteroides fragilis ethyl 2-oxo-2-phenylacetate + NADH
-
?
methyl 2-hydroxy-2-phenylacetate + NAD+
-
Bacteroides fragilis methyl 2-oxo-2-phenylacetate + NADH
-
?
methyl benzoylformate + NADH
-
Bacteroides fragilis ?
-
?
additional information substrate specificity and stereoselectivity towards various ketones, product determination, overview Bacteroides fragilis ?
-
?

Synonyms

Synonyms Comment Organism
7-HSDH
-
Bacteroides fragilis
7alpha-hydroxysteroid dehydrogenase
-
Bacteroides fragilis

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
22
-
assay at room temperature Bacteroides fragilis

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
additional information
-
the enzyme is thermostable Bacteroides fragilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Bacteroides fragilis

Cofactor

Cofactor Comment Organism Structure
NADH
-
Bacteroides fragilis