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Literature summary for extracted from

  • Coleman, J.P.; Hudson, L.L.; Adams, M.J.
    Characterization and regulation of the NADP-linked 7alpha-hydroxysteroid dehydrogenase gene from Clostridium sordellii (1994), J. Bacteriol., 176, 4865-4874.
    View publication on PubMedView publication on EuropePMC


Cloned (Comment) Organism
expression in Escherichia coli DH5alpha-MCR, DNA and amino acid sequence analysis Paeniclostridium sordellii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
? * 30000, SDS-PAGE Paeniclostridium sordellii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Paeniclostridium sordellii bile acid-inducible to 10fold higher activity on substrate compared to uninduced cells ?


Organism UniProt Comment Textmining
Paeniclostridium sordellii

Purification (Commentary)

Purification (Comment) Organism
Paeniclostridium sordellii


Reaction Comment Organism Reaction ID
cholate + NAD+ = 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oate + NADH + H+ short-chain dehydrogenase group Paeniclostridium sordellii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
crude extract Paeniclostridium sordellii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chenodeoxycholic acid + NAD(P)+ inducible by growth on bile acids, 10fold higher enzyme activity on substrate than without induction Paeniclostridium sordellii 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NAD(P)H
additional information bile acid-inducible to 10fold higher activity on substrate compared to uninduced cells Paeniclostridium sordellii ?


Subunits Comment Organism
? ? * 30000, SDS-PAGE Paeniclostridium sordellii


Cofactor Comment Organism Structure
NAD+ recombinant enzyme from E. coli Paeniclostridium sordellii
Paeniclostridium sordellii