General Stability | Organism |
---|---|
the relative activity of immobilized enzyme on aminoepoxy resin increases by approximately 80%. After 6 cycles, the residual activities of the enzyme immobilized on the amino-epoxy resin and epoxy resin are 81.4% and 89.5%, respectively | Clostridium sardiniense |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
taurochenodeoxycholic acid + NADP+ | Clostridium sardiniense | - |
tauro-7-ketolithocholic acid + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium sardiniense | G9FRD7 | - |
- |
Storage Stability | Organism |
---|---|
4°C, 50 mM Tris-HCl (pH 9.0), 2 weeks, 24.9% loss of activity, immobilized enzyme | Clostridium sardiniense |
4°C, 50 mM Tris-HCl (pH 9.0), 5 weeks, 60.3% loss of activity, immobilized enzyme | Clostridium sardiniense |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
taurochenodeoxycholic acid + NADP+ | - |
Clostridium sardiniense | tauro-7-ketolithocholic acid + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
7alpha-HSDH | - |
Clostridium sardiniense |
nicotinamide adenine dinucleotide phosphate-dependent 7alpha-hydroxysteroid dehydrogenase | - |
Clostridium sardiniense |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Clostridium sardiniense |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 65 | the relative activity of the free enzyme drastically decreases at temperatures over 35°C, with minimal activity observed at 45°C. In contrast, the immobilized preparations are relatively stable at temperatures ranging from 15 to 35°C, with the relative activities of enzyme immobilized on the amino-epoxy resin and epoxy resin at 56.4% and 61.0%, respectively, after 2 h at 65°C | Clostridium sardiniense |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Clostridium sardiniense |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9 | - |
the optimum conditions for the immobilization of the enzyme on both amino-epoxy resin and epoxy resin are pH 9.0 and 20°C for 2 h | Clostridium sardiniense |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Clostridium sardiniense |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Clostridium sardiniense | isoelectric focusing | - |
5.7 |