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Literature summary for extracted from

  • Liu, J.J.; Zhang, G.C.; Kwak, S.; Oh, E.J.; Yun, E.J.; Chomvong, K.; Cate, J.H.D.; Jin, Y.S.
    Overcoming the thermodynamic equilibrium of an isomerization reaction through oxidoreductive reactions for biotransformation (2019), Nat. Commun., 10, 1356 .
    View publication on PubMedView publication on EuropePMC


Application Comment Organism
synthesis a yeast strain capable of consuming lactose intracellularly is engineered to produce tagatose from lactose. GAL1 coding for galactose kinase is deleted to eliminate galactose utilization. Heterologous xylose reductase (XR) and galactitol dehydrogenase (GDH) are introduced into the Gal1 deletion strain. The expression levels of XR and GDH are adjusted to maximize tagatose production. The resulting engineered yeast produces 37.69 g/l of tagatose from lactose with a tagatose and galactose ratio of 9:1 in the reaction broth Rhizobium leguminosarum bv. viciae


Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Rhizobium leguminosarum bv. viciae


Organism UniProt Comment Textmining
Rhizobium leguminosarum bv. viciae Q1MLL4


Synonyms Comment Organism
Rhizobium leguminosarum bv. viciae