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Literature summary for 1.1.1.164 extracted from

  • Finnerty, W.R.
    Primary alcohol dehydrogenases from Acinetobacter (1990), Methods Enzymol., 188, 14-18.
    View publication on PubMed

General Stability

General Stability Organism
highly unstable, more than 50% loss of activity within 24 h Acinetobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
rifampicin inhibits enzyme induction in hexadecanol-grown cells, not in cells grown on ethanol, palmitate or hexadecane Acinetobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
hexadecanol growth on ethanol, NAD+ Acinetobacter sp.
0.0073
-
hexadecanol growth on palmitic acid, NAD+ Acinetobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hexadecanal + NADH Acinetobacter sp. inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane hexadecanol + NAD+
-
?
hexadecanal + NADH Acinetobacter sp. HO1-N inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane hexadecanol + NAD+
-
?

Organism

Organism UniProt Comment Textmining
Acinetobacter sp.
-
-
-
Acinetobacter sp. HO1-N
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Acinetobacter sp.

Storage Stability

Storage Stability Organism
-20Ā°C, Tris-HCl 10 mM, pH 7.8, precipitation at 50% ammonium sulfate saturation, dissolved in 15fold reduced volume Acinetobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hexadecanal + NADH inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane Acinetobacter sp. hexadecanol + NAD+
-
?
hexadecanal + NADH inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane Acinetobacter sp. HO1-N hexadecanol + NAD+
-
?
additional information enzyme is not visible as enzymatically active protein in zymograms Acinetobacter sp. ?
-
?
additional information enzyme is not visible as enzymatically active protein in zymograms Acinetobacter sp. HO1-N ?
-
?

Cofactor

Cofactor Comment Organism Structure
NAD+ preference for Acinetobacter sp.