BRENDA - Enzyme Database
show all sequences of 1.1.1.168

Discovery of a new NADPH-dependent aldo-keto reductase from Candida orthopsilosis catalyzing the stereospecific synthesis of (R)-pantolactone by genome mining

Wang, J.; Cheng, P.; Wu, Y.; Wang, A.; Liu, F.; Pei, X.; J. Biotechnol. 291, 26-34 (2019) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3) cells
Candida orthopsilosis
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ag+
complete inhibition at 1 mM
Candida orthopsilosis
Co2+
81.9% residual activity at 1 mM
Candida orthopsilosis
Cu2+
67.8% residual activity at 1 mM
Candida orthopsilosis
EDTA
-
Candida orthopsilosis
Fe2+
81% residual activity at 1 mM
Candida orthopsilosis
Mg2+
80.2% residual activity at 1 mM
Candida orthopsilosis
Mn2+
67.6% residual activity at 1 mM
Candida orthopsilosis
Pb2+
complete inhibition at 1 mM
Candida orthopsilosis
Zn2+
71.7% residual activity at 1 mM
Candida orthopsilosis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3
-
2-dehydropantolactone
at pH 7.0 and 30°C
Candida orthopsilosis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
1 mM Ni2+ and Ca2+ have almost no influence over the enzyme activity
Candida orthopsilosis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-dehydropantolactone + NADPH + H+
Candida orthopsilosis
-
(R)-pantolactone + NADP+
-
-
?
2-dehydropantolactone + NADPH + H+
Candida orthopsilosis 90-125
-
(R)-pantolactone + NADP+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Candida orthopsilosis
H8XA83
-
-
Candida orthopsilosis 90-125
H8XA83
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Ni-NTA column chromatography
Candida orthopsilosis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-dehydropantolactone + NADPH + H+
-
761570
Candida orthopsilosis
(R)-pantolactone + NADP+
-
-
-
?
2-dehydropantolactone + NADPH + H+
100% activity, 99% conversion and more than 99% enantiomeric excess within 5 h
761570
Candida orthopsilosis
(R)-pantolactone + NADP+
-
-
-
?
2-dehydropantolactone + NADPH + H+
-
761570
Candida orthopsilosis 90-125
(R)-pantolactone + NADP+
-
-
-
?
2-dehydropantolactone + NADPH + H+
100% activity, 99% conversion and more than 99% enantiomeric excess within 5 h
761570
Candida orthopsilosis 90-125
(R)-pantolactone + NADP+
-
-
-
?
ethyl 2-oxopropanoate + NADPH + H+
0.496% activity compared to 2-dehydropantolactone
761570
Candida orthopsilosis
ethyl (2R)-2-hydroxypropanoate + NADP+
-
-
-
?
ethyl 2-oxopropanoate + NADPH + H+
0.496% activity compared to 2-dehydropantolactone
761570
Candida orthopsilosis 90-125
ethyl (2R)-2-hydroxypropanoate + NADP+
-
-
-
?
ethyl oxo(phenyl)acetate + NADPH + H+
3.45% activity compared to 2-dehydropantolactone
761570
Candida orthopsilosis
ethyl (2R)-hydroxy(phenyl)acetate + NADP+
-
-
-
?
ethyl oxo(phenyl)acetate + NADPH + H+
3.45% activity compared to 2-dehydropantolactone
761570
Candida orthopsilosis 90-125
ethyl (2R)-hydroxy(phenyl)acetate + NADP+
-
-
-
?
methyl 2-oxopropanoate + NADPH + H+
0.501% activity compared to 2-dehydropantolactone
761570
Candida orthopsilosis
methyl (2R)-2-hydroxypropanoate + NADP+
-
-
-
?
methyl oxo(phenyl)acetate + NADPH + H+
3.94% activity compared to 2-dehydropantolactone
761570
Candida orthopsilosis
methyl (2R)-hydroxy(phenyl)acetate + NADP+
-
-
-
?
additional information
the enzyme does not reduce 4-nitrobenzaldehyde
761570
Candida orthopsilosis
?
-
-
-
-
additional information
the enzyme does not reduce 4-nitrobenzaldehyde
761570
Candida orthopsilosis 90-125
?
-
-
-
-
pyridine 3-aldehyde + NADPH + H+
0.538% activity compared to 2-dehydropantolactone
761570
Candida orthopsilosis
(pyridin-3-yl)methanol + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 37000, His6-tagged enyzme, SDS-PAGE
Candida orthopsilosis
Synonyms
Synonyms
Commentary
Organism
AKR
-
Candida orthopsilosis
NADPH-dependent aldo-keto reductase
-
Candida orthopsilosis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Candida orthopsilosis
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
55
the activity remains above 50% of the maximum activity between 30 and 55°C
Candida orthopsilosis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
50
the recombinant enzyme exhibits high thermal stability with half-lives of 6.3 h, 3.7 h and 52 min at 40, 45 and 50°C, respectively
Candida orthopsilosis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
-
Candida orthopsilosis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
8
more than 40% activity between pH 6.0 and 8.0
Candida orthopsilosis
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Candida orthopsilosis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Candida orthopsilosis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Candida orthopsilosis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ag+
complete inhibition at 1 mM
Candida orthopsilosis
Co2+
81.9% residual activity at 1 mM
Candida orthopsilosis
Cu2+
67.8% residual activity at 1 mM
Candida orthopsilosis
EDTA
-
Candida orthopsilosis
Fe2+
81% residual activity at 1 mM
Candida orthopsilosis
Mg2+
80.2% residual activity at 1 mM
Candida orthopsilosis
Mn2+
67.6% residual activity at 1 mM
Candida orthopsilosis
Pb2+
complete inhibition at 1 mM
Candida orthopsilosis
Zn2+
71.7% residual activity at 1 mM
Candida orthopsilosis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3
-
2-dehydropantolactone
at pH 7.0 and 30°C
Candida orthopsilosis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
1 mM Ni2+ and Ca2+ have almost no influence over the enzyme activity
Candida orthopsilosis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-dehydropantolactone + NADPH + H+
Candida orthopsilosis
-
(R)-pantolactone + NADP+
-
-
?
2-dehydropantolactone + NADPH + H+
Candida orthopsilosis 90-125
-
(R)-pantolactone + NADP+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography
Candida orthopsilosis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-dehydropantolactone + NADPH + H+
-
761570
Candida orthopsilosis
(R)-pantolactone + NADP+
-
-
-
?
2-dehydropantolactone + NADPH + H+
100% activity, 99% conversion and more than 99% enantiomeric excess within 5 h
761570
Candida orthopsilosis
(R)-pantolactone + NADP+
-
-
-
?
2-dehydropantolactone + NADPH + H+
-
761570
Candida orthopsilosis 90-125
(R)-pantolactone + NADP+
-
-
-
?
2-dehydropantolactone + NADPH + H+
100% activity, 99% conversion and more than 99% enantiomeric excess within 5 h
761570
Candida orthopsilosis 90-125
(R)-pantolactone + NADP+
-
-
-
?
ethyl 2-oxopropanoate + NADPH + H+
0.496% activity compared to 2-dehydropantolactone
761570
Candida orthopsilosis
ethyl (2R)-2-hydroxypropanoate + NADP+
-
-
-
?
ethyl 2-oxopropanoate + NADPH + H+
0.496% activity compared to 2-dehydropantolactone
761570
Candida orthopsilosis 90-125
ethyl (2R)-2-hydroxypropanoate + NADP+
-
-
-
?
ethyl oxo(phenyl)acetate + NADPH + H+
3.45% activity compared to 2-dehydropantolactone
761570
Candida orthopsilosis
ethyl (2R)-hydroxy(phenyl)acetate + NADP+
-
-
-
?
ethyl oxo(phenyl)acetate + NADPH + H+
3.45% activity compared to 2-dehydropantolactone
761570
Candida orthopsilosis 90-125
ethyl (2R)-hydroxy(phenyl)acetate + NADP+
-
-
-
?
methyl 2-oxopropanoate + NADPH + H+
0.501% activity compared to 2-dehydropantolactone
761570
Candida orthopsilosis
methyl (2R)-2-hydroxypropanoate + NADP+
-
-
-
?
methyl oxo(phenyl)acetate + NADPH + H+
3.94% activity compared to 2-dehydropantolactone
761570
Candida orthopsilosis
methyl (2R)-hydroxy(phenyl)acetate + NADP+
-
-
-
?
additional information
the enzyme does not reduce 4-nitrobenzaldehyde
761570
Candida orthopsilosis
?
-
-
-
-
additional information
the enzyme does not reduce 4-nitrobenzaldehyde
761570
Candida orthopsilosis 90-125
?
-
-
-
-
pyridine 3-aldehyde + NADPH + H+
0.538% activity compared to 2-dehydropantolactone
761570
Candida orthopsilosis
(pyridin-3-yl)methanol + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 37000, His6-tagged enyzme, SDS-PAGE
Candida orthopsilosis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Candida orthopsilosis
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
55
the activity remains above 50% of the maximum activity between 30 and 55°C
Candida orthopsilosis
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
50
the recombinant enzyme exhibits high thermal stability with half-lives of 6.3 h, 3.7 h and 52 min at 40, 45 and 50°C, respectively
Candida orthopsilosis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
-
Candida orthopsilosis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
8
more than 40% activity between pH 6.0 and 8.0
Candida orthopsilosis
Other publictions for EC 1.1.1.168
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
761008
Cheng
Recombinant expression and mo ...
Candida dubliniensis, Candida dubliniensis CD36
Enzyme Microb. Technol.
126
77-85
2019
-
-
1
-
3
-
11
3
-
-
-
2
-
12
-
-
1
-
-
-
3
-
2
1
2
1
1
1
3
1
-
1
1
-
-
-
-
-
1
1
-
3
-
-
11
-
3
-
-
-
2
-
-
-
1
-
-
3
-
2
1
1
1
1
3
1
-
1
-
-
-
-
-
3
3
761570
Wang
Discovery of a new NADPH-depe ...
Candida orthopsilosis, Candida orthopsilosis 90-125
J. Biotechnol.
291
26-34
2019
-
-
1
-
-
-
9
1
-
1
-
2
-
6
-
-
1
-
-
-
-
-
13
1
2
1
1
1
-
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
9
-
1
-
1
-
2
-
-
-
1
-
-
-
-
13
1
1
1
1
-
1
1
-
-
-
-
-
-
-
-
347731
Julliard
-
Purification and characterizat ...
Spinacia oleracea
Bot. Acta
107
191-200
1994
-
-
-
-
-
-
3
4
-
-
1
-
-
1
-
-
1
-
-
-
-
1
6
1
-
-
-
1
-
1
-
-
1
1
-
-
-
-
-
1
-
-
-
-
3
1
4
-
-
1
-
-
-
-
1
-
-
-
1
6
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
347730
Kataoka
Enzymes involved in the NADPH ...
Saccharomyces cerevisiae, Candida parapsilosis, Mucor ambiguus, Mucor ambiguus AKU 3006
Biosci. Biotechnol. Biochem.
56
820-821
1992
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
7
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
347723
Hata
Ketopantoyl-lactone reductase ...
Saccharomyces cerevisiae
FEMS Microbiol. Lett.
58
87-90
1989
-
-
-
-
-
-
3
16
-
-
-
1
-
4
-
-
-
-
-
-
-
-
21
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
16
-
-
-
1
-
-
-
-
-
-
-
-
21
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347724
Hata
Ketopantoyl-lactone reductase ...
Candida parapsilosis
Biochim. Biophys. Acta
990
175-181
1989
-
-
-
1
-
-
11
4
-
-
2
2
-
10
-
-
1
-
-
-
2
-
12
1
-
1
-
2
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
12
-
4
-
-
2
2
-
-
-
1
-
-
2
-
12
1
1
-
2
-
-
-
2
-
-
-
-
-
-
-
347726
Wilken
Ketopantoyl lactone reductases ...
Saccharomyces cerevisiae
Methods Enzymol.
62
209-215
1979
-
-
-
-
-
1
5
-
-
-
1
1
-
1
-
-
1
-
-
-
1
1
4
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
1
1
-
-
-
1
-
-
1
1
4
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
347725
Wilken
Stereospecificity of pantoyl l ...
Saccharomyces cerevisiae
Arch. Biochem. Biophys.
189
251-255
1978
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286105
Wilken
Ketopantoic acid and ketopanto ...
Saccharomyces cerevisiae
J. Biol. Chem.
250
2311-2314
1975
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347727
King
Ketopantoyl lactone and ketopa ...
Saccharomyces cerevisiae
J. Biol. Chem.
249
4689-4695
1974
-
-
-
-
-
-
-
4
-
-
1
1
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
1
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286104
King
-
Separation and preliminary stu ...
Saccharomyces cerevisiae
J. Biol. Chem.
247
4096-4098
1972
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-