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Literature summary for 1.1.1.17 extracted from

  • Brown, A.T.; Bowles, R.D.
    Polyol metabolism by a caries-conductive Streptococcus: Purification and properties of a nicotinamide adenine dinucleotide-dependent mannitol-1-phosphate dehydrogenase (1977), Infect. Immun., 16, 163-173.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
adenosine 48% inhibition of fructose 6-phosphate reduction Streptococcus mutans
ADP 20% inhibition of mannitol 1-phosphate oxidation Streptococcus mutans
ATP 53% inhibition of mannitol 1-phosphate oxidation Streptococcus mutans
cytidine 17% inhibition of fructose 6-phosphate reduction Streptococcus mutans
D-fructose 1,6-diphosphate 12% inhibition of mannitol 1-phosphate oxidation Streptococcus mutans
D-fructose 6-phosphate 56% inhibition of mannitol 1-phosphate oxidation Streptococcus mutans
GTP 11% inhibition of mannitol 1-phosphate oxidation Streptococcus mutans
guanosine 10% inhibition of fructose 6-phosphate reduction Streptococcus mutans
phosphoenolpyruvate 14% inhibition of mannitol 1-phosphate oxidation Streptococcus mutans
thymidine 8% inhibition of fructose 6-phosphate reduction Streptococcus mutans
uridine 16% inhibition of fructose 6-phosphate reduction Streptococcus mutans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
NADH cosubstrate: D-fructose 6-phosphate Streptococcus mutans
0.06
-
NAD+ cosubstrate: D-mannitol 1-phosphate Streptococcus mutans
0.15
-
D-mannitol 1-phosphate cosubstrate: NAD+ Streptococcus mutans
1.66
-
D-fructose 6-phosphate cosubstrate: NADH Streptococcus mutans

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
Streptococcus mutans 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
gel filtration Streptococcus mutans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-mannitol 1-phosphate + NAD+ Streptococcus mutans highly specific for both substrates D-fructose 6-phosphate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Streptococcus mutans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps, affinity chromatography Streptococcus mutans

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Streptococcus mutans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.43
-
-
Streptococcus mutans

Storage Stability

Storage Stability Organism
-80°C, 0.05 M Tris-HCl, pH 7.5, presence of 2-mercaptoethanol, long term storage without loss of activity Streptococcus mutans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-mannitol 1-phosphate + NAD+ highly specific for both substrates Streptococcus mutans D-fructose 6-phosphate + NADH + H+
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
reduction of D-fructose 6-phosphate Streptococcus mutans
8.5 9 oxidation of D-mannitol 1-phosphate Streptococcus mutans

Cofactor

Cofactor Comment Organism Structure
NAD+ strictly specific Streptococcus mutans
NADH
-
Streptococcus mutans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2
-
ADP competitive inhibition with respect to NADH, noncompetitive inhibition with respect to fructose 6-phosphate Streptococcus mutans
2.2
-
ATP strong inhibition of mannitol 1-phosphate oxidation Streptococcus mutans
3.33
-
D-fructose 6-phosphate able to inhibit the forward reaction to 100%, competitive inhibition with respect to mannitol 1-phosphate, noncompetitive inhibition with respect to NAD+ Streptococcus mutans