Literature summary for 1.1.1.17 extracted from

Krahulec, S.; Armao, G.C.; Weber, H.; Klimacek, M.; Nidetzky, B.
Characterization of recombinant Aspergillus fumigatus mannitol-1-phosphate 5-dehydrogenase and its application for the stereoselective synthesis of protio and deuterio forms of D-mannitol 1-phosphate (2008), Carbohydr. Res., 343, 1414-1423.

Search for more literature for 1.1.1.17

Application

Application	Comment	Organism
synthesis	hydrogen transfer from formate to D-fructose 6-phosphate, mediated by NAD(H) and catalyzed by a coupled enzyme system of purified Candida boidinii formate dehydrogenase and AfM1PDH, is used for the preparative synthesis of D-mannitol 1-phosphate or, by applying an analogous procedure using deuterio formate, the 5-[2H] derivative thereof, overview	Aspergillus fumigatus

Cloned(Commentary)

Cloned (Comment)	Organism
high level overexpression in Escherichia coli strain JM109	Aspergillus fumigatus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetic isotope effects, overview	Aspergillus fumigatus
0.016	-	NADH	pH 7.1, 25°C, recombinant enzyme	Aspergillus fumigatus
0.23	-	D-mannitol 1-phosphate	pH 7.1, 25°C, recombinant enzyme	Aspergillus fumigatus
0.75	-	NAD+	pH 7.1, 25°C, recombinant enzyme	Aspergillus fumigatus
2.1	-	D-fructose 6-phosphate	pH 7.1, 25°C, recombinant enzyme	Aspergillus fumigatus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	x * 40000, recombinant enzyme, SDS-PAGE	Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-mannitol 1-phosphate + NAD+	Aspergillus fumigatus	-	D-fructose 6-phosphate + NADH + H+	-	r

Organism

Organism	UniProt	Comment	Textmining
Aspergillus fumigatus	Q4X1A4	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme 2.1fold from Escherichia coli by affinity chromatography	Aspergillus fumigatus

Reaction

Reaction	Comment	Organism	Reaction ID
D-mannitol 1-phosphate + NAD+ = D-fructose 6-phosphate + NADH + H+	the hydride transfer is strongly rate-determining for the overall enzymatic reaction	Aspergillus fumigatus	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
17.6	-	oxidation reaction, purified recombinant enzyme	Aspergillus fumigatus
169	-	reduction reaction, purified recombinant enzyme	Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-mannitol 1-phosphate + NAD+	-	Aspergillus fumigatus	D-fructose 6-phosphate + NADH + H+	-	r
D-mannitol 1-phosphate + NAD+	M1PDH is stereospecific for transferring the hydrogen to NAD+, in situ proton NMR studies of enzymatic oxidation of D-5-[2H]-mannitol 1-phosphate, overview	Aspergillus fumigatus	D-fructose 6-phosphate + NADH + H+	-	r

Subunits

Subunits	Comment	Organism
?	x * 40000, recombinant enzyme, SDS-PAGE	Aspergillus fumigatus

Synonyms

Synonyms	Comment	Organism
long-chain mannitol-1-phosphate 5-dehydrogenase	-	Aspergillus fumigatus
M1PDH	-	Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	-	Aspergillus fumigatus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
8.5	-	D-mannitol 1-phosphate	pH 7.1, 25°C, recombinant enzyme	Aspergillus fumigatus
125	-	D-fructose 6-phosphate	pH 7.1, 25°C, recombinant enzyme	Aspergillus fumigatus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.1	-	-	Aspergillus fumigatus

Cofactor

Cofactor	Comment	Organism	Structure
additional information	poor activity with NADP(H)	Aspergillus fumigatus
NAD+	dependent on	Aspergillus fumigatus
NADH	dependent on	Aspergillus fumigatus

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Release 2023.1 (January 2023)