Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.175 extracted from

  • Nygard, Y.; Maaheimo, H.; Mojzita, D.; Toivari, M.; Wiebe, M.; Resnekov, O.; Gustavo Pesce, C.; Ruohonen, L.; Penttilae, M.
    Single cell and in vivo analyses elucidate the effect of xylC lactonase during production of D-xylonate in Saccharomyces cerevisiae (2014), Metab. Eng., 25, 238-247.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene xylB, recombinnat expression in Saccharomyces cerevisiae strain VVT B-67002 Caulobacter vibrioides

Protein Variants

Protein Variants Comment Organism
additional information both D-xylono-gamma-lactone and D-xylonate are produced when the Caulobacter crescentus gene xylB encoding D-xylose dehydrogenase is expressed in Saccharomyces cerevisiae, with or without co-expressionof xylC (D-xylonolactonase). XylC facilitates rapid opening of the lactone and more D-xylonate is initially produced than in its absence. In vivo [1H]NMR analysis of cell extracts, culture media and intact cells is used for analysis. The lactone and linear forms of D-xylonic acid are produced, accumulated intracellularly, and partially exported within 15┬ľ60 min after D-xylose provision. Co-expression of xylB and xylC leads to rapid loss of pHluorin fluorescence and loss of vitality during production of D-xylonate. Loss of vitality in the presence of D-xylose is correlated to the extracellular pH, but fluorescence is lost from xylB and xylC expressing cells regardless of the extracellular condition. Method optimization, overview Caulobacter vibrioides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-xylose + NAD+ Caulobacter vibrioides
-
D-xylonolactone + NADH + H+
-
?
D-xylose + NAD+ Caulobacter vibrioides NA1000
-
D-xylonolactone + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Caulobacter vibrioides B8H1Z0
-
-
Caulobacter vibrioides NA1000 B8H1Z0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-xylose + NAD+
-
Caulobacter vibrioides D-xylonolactone + NADH + H+
-
?
D-xylose + NAD+
-
Caulobacter vibrioides NA1000 D-xylonolactone + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
D-xylose 1-dehydrogenase
-
Caulobacter vibrioides
XylB
-
Caulobacter vibrioides

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
30
-
in vivo assay, cell growth at 30┬░C Caulobacter vibrioides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
assay at Caulobacter vibrioides

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Caulobacter vibrioides