Reference to 1.1.1.179; Id = 286127 - BRENDA Enzyme Database

additional information

-

Macaca fuscata

cDNA subcloned in expression vector pRset, overexpression in Escherichia coli

Macaca fuscata

H79Q

increase in Km more than 25fold, increase in Kd more than 7fold, with high ionic strength Km increased by 190fold and inhibited the enzyme

Macaca fuscata

Y180F

almost inactive, high affinity for NADP(H)

Macaca fuscata

high salt concentration

recombinant H79Q mutant

Macaca fuscata

additional information

-

additional information

phosphate buffer increases the Km-value, especially for mutant H79Q, compared with Tris buffer

Macaca fuscata

0.0006

-

NADPH

camphorquinone

Macaca fuscata

0.0006

-

NADP+

D-xylose

Macaca fuscata

0.014

-

NADP+

D-xylose, mutant H79Q

Macaca fuscata

0.028

-

NADPH

camphorquinone, mutant H79Q

Macaca fuscata

0.1

-

Camphorquinone

-

Macaca fuscata

0.51

-

Camphorquinone

mutant H79Q

Macaca fuscata

4.4

-

D-xylose

-

Macaca fuscata

67

-

D-xylose

mutant H79Q

Macaca fuscata

36000

-

2 * 36000, SDS-PAGE

Macaca fuscata

70000

-

about, gel filtration

Macaca fuscata

-

recombinant enzyme and mutants Y180F, H79Q

Macaca fuscata

kidney

-

Macaca fuscata

-

5.3

5.5

wild-type and mutant H79Q

Macaca fuscata

18

-

substrate camphorquinone, mutant H79Q

Macaca fuscata

33

-

substrate camphorquinone

Macaca fuscata

camphorquinone + NADPH

-

286127

Macaca fuscata

(1R)-1,7,7-trimethylbicyclo[2.2.1]heptane-2,3-diol + NADP+

-

?

D-xylose + NADP+

-

286127

Macaca fuscata

D-xylono-1,5-lactone + NADPH + H+

-

?

additional information

catalyzing oxidation of trans-dihydrodiols of aromatic hydrocarbons to corresponding catechols, probably identical with dimeric dihydrodiol dehydrogenase EC 1.3.1.20

286127

Macaca fuscata

?

-

?

dimer

2 * 36000, SDS-PAGE

Macaca fuscata

NADP+

-

Macaca fuscata

additional information

-

Macaca fuscata

cDNA subcloned in expression vector pRset, overexpression in Escherichia coli

Macaca fuscata

NADP+

-

Macaca fuscata

H79Q

increase in Km more than 25fold, increase in Kd more than 7fold, with high ionic strength Km increased by 190fold and inhibited the enzyme

Macaca fuscata

Y180F

almost inactive, high affinity for NADP(H)

Macaca fuscata

high salt concentration

recombinant H79Q mutant

Macaca fuscata

additional information

-

additional information

phosphate buffer increases the Km-value, especially for mutant H79Q, compared with Tris buffer

Macaca fuscata

0.0006

-

NADPH

camphorquinone

Macaca fuscata

0.0006

-

NADP+

D-xylose

Macaca fuscata

0.014

-

NADP+

D-xylose, mutant H79Q

Macaca fuscata

0.028

-

NADPH

camphorquinone, mutant H79Q

Macaca fuscata

0.1

-

Camphorquinone

-

Macaca fuscata

0.51

-

Camphorquinone

mutant H79Q

Macaca fuscata

4.4

-

D-xylose

-

Macaca fuscata

67

-

D-xylose

mutant H79Q

Macaca fuscata

36000

-

2 * 36000, SDS-PAGE

Macaca fuscata

70000

-

about, gel filtration

Macaca fuscata

recombinant enzyme and mutants Y180F, H79Q

Macaca fuscata

kidney

-

Macaca fuscata

-

5.3

5.5

wild-type and mutant H79Q

Macaca fuscata

18

-

substrate camphorquinone, mutant H79Q

Macaca fuscata

33

-

substrate camphorquinone

Macaca fuscata

camphorquinone + NADPH

-

286127

Macaca fuscata

(1R)-1,7,7-trimethylbicyclo[2.2.1]heptane-2,3-diol + NADP+

-

?

D-xylose + NADP+

-

286127

Macaca fuscata

D-xylono-1,5-lactone + NADPH + H+

-

?

additional information

catalyzing oxidation of trans-dihydrodiols of aromatic hydrocarbons to corresponding catechols, probably identical with dimeric dihydrodiol dehydrogenase EC 1.3.1.20

286127

Macaca fuscata

?

-

?

dimer

2 * 36000, SDS-PAGE

Macaca fuscata

723749

Mihasan

Evidence of a plasmid-encoded ...

Paenarthrobacter nicotinovorans

Res. Microbiol.

164

22-30

2013

-

1

-

3

-

1

-

1

-

3

1

-

3

-

2

-

1

2

-

3

-

1

-

3

1

-

3

-

3

724150

Ahmed

-

Oxidation and reduction of D-x ...

Ogataea angusta

Aust. J. Basic Appl. Sci.

5

95-100

2011

-

2

-

1

-

3

-

2

-

2

-

2

-

3

-

725867

Nygard

Bioconversion of d-xylose to d ...

Trichoderma reesei

Metab. Eng.

13

383-391

2011

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

698940

Johnsen

D-xylose degradation pathway i ...

Haloferax volcanii, Haloferax volcanii DS2

J. Biol. Chem.

284

27290-27303

2009

1

-

2

-

1

-

6

-

3

2

-

3

-

1

-

1

3

-

10

1

2

-

2

-

1

-

2

-

1

-

6

-

3

2

-

1

-

1

3

-

10

1

-

684150

Carbone

Structure of monkey dimeric di ...

Macaca fascicularis

Acta Crystallogr. Sect. D

64

532-542

2008

-

1

15

-

2

13

-

2

-

2

-

1

-

2

1

-

8

1

-

1

-

1

15

-

2

-

13

-

2

-

1

-

2

1

-

8

1

-

686797

Berghaell

Identification in the mould Hy ...

Trichoderma reesei

FEMS Microbiol. Lett.

277

249-253

2007

-

1

-

4

-

1

-

1

-

1

-

1

-

6

1

2

1

-

2

1

-

1

-

1

-

4

-

1

-

1

-

1

-

6

1

-

2

1

-

655884

Johnsen

Novel xylose dehydrogenase in ...

Haloarcula marismortui

J. Bacteriol.

186

6198-6207

2004

-

1

-

3

-

3

2

1

-

1

-

1

-

1

-

5

1

-

2

1

-

3

1

-

2

-

1

2

-

3

-

3

2

1

-

1

-

1

-

5

1

2

1

-

3

1

-

3

286128

Aoki

Identity of dimeric dihydrodio ...

Canis lupus familiaris, Oryctolagus cuniculus, Homo sapiens, Macaca fuscata, Sus scrofa

Chem. Biol. Interact.

130-132

775-784

2001

4

-

2

-

1

4

-

5

-

16

-

4

1

-

8

2

-

18

-

8

-

7

-

4

-

7

-

2

-

1

-

4

-

5

-

4

-

8

2

-

18

-

8

-

286127

Asada

Roles of His-79 and Tyr-180 of ...

Macaca fuscata

Biochem. Biophys. Res. Commun.

278

333-337

2000

1

-

1

-

2

-

1

9

-

2

-

1

-

1

-

1

3

-

3

1

-

1

-

1

-

1

-

2

-

1

-

9

-

2

-

1

-

1

3

-

3

1

-

286126

Zepeda

NADP(+)-dependent D-xylose deh ...

Sus scrofa

Biochem. J.

266

637-644

1990

-

2

9

-

2

-

1

-

1

-

1

-

8

1

-

1

-

7

1

-

2

-

2

-

2

-

9

-

2

-

1

-

1

-

8

1

-

7

1

-

286124

Wissler

-

D-Xylose:NADP+ oxidoreductase ...

Bos taurus, Canis lupus familiaris, Sus scrofa

Hoppe-Seyler's Z. Physiol. Chem.

358

1300-1301

1977

-

3

-

3

-

7

-

24

-

3

-

3

-

3

-

7

-

24

-

286125

Suzuki

Oxidation and reduction of D-x ...

Starmera quercuum

Appl. Microbiol.

25

850-852

1973

-

1

-

1

-

1

-

1

-

1

-

Roles of His-79 and Tyr-180 of D-xylose/dihydrodiol dehydrogenase in catalytic function

Data extracted from this reference: