Reference to 1.1.1.179; Id = 684150 - BRENDA Enzyme Database

wild-type protein and mutants are expressed in Escherichia coli BL21 (DE3)

Macaca fascicularis

crystals are obtained at 19.9Â°C in a culture plate via the vapour-diffusion method, crystal structure of dimeric D-xylose dehydrogenase complexed with the inhibitor isoascorbic acid is determined at 2.59 A resolution

Macaca fascicularis

A36D

site-directed mutagenesis

Macaca fascicularis

D280A

by site-directed mutagenesis

Macaca fascicularis

F154A

by site-directed mutagenesis

Macaca fascicularis

F279A

by site-directed mutagenesis

Macaca fascicularis

H76Q

by site-directed mutagenesis

Macaca fascicularis

H79Q

by site-directed mutagenesis

Macaca fascicularis

K97M

by site-directed mutagenesis

Macaca fascicularis

K97R

by site-directed mutagenesis

Macaca fascicularis

R37A

site-directed mutagenesis

Macaca fascicularis

R37D

site-directed mutagenesis

Macaca fascicularis

R41A

by site-directed mutagenesis

Macaca fascicularis

R41D

by site-directed mutagenesis

Macaca fascicularis

W125Y

by site-directed mutagenesis

Macaca fascicularis

W254A

by site-directed mutagenesis

Macaca fascicularis

W254Y

by site-directed mutagenesis

Macaca fascicularis

4-hydroxyacetophenone

-

Macaca fascicularis

isoascorbic acid

competitive inhibitor

Macaca fascicularis

2.4

-

D-xylose

oxidation reaction of mutant R41A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

Macaca fascicularis

2.9

-

D-xylose

oxidation reaction of mutant R41D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

Macaca fascicularis

3.4

-

D-xylose

oxidation reaction of mutant R37A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

Macaca fascicularis

4.4

-

D-xylose

oxidation reaction of wild type enzyme, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

Macaca fascicularis

8

9

D-xylose

oxidation reaction of mutant F279A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

Macaca fascicularis

11

-

D-xylose

oxidation reaction of mutant R37D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

Macaca fascicularis

14

-

D-xylose

oxidation reaction of mutant W254Y, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

Macaca fascicularis

15

-

D-xylose

oxidation reaction of mutant H79Q, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

Macaca fascicularis

36

-

D-xylose

oxidation reaction of mutant F154A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

Macaca fascicularis

41

-

D-xylose

oxidation reaction of mutant H76Q, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

Macaca fascicularis

65

-

D-xylose

oxidation reaction of mutant A36D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

Macaca fascicularis

67

-

D-xylose

oxidation reaction of mutant W125Y, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

Macaca fascicularis

90

-

D-xylose

oxidation reaction of mutant W254A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

Macaca fascicularis

D-xylose + NADP+

Macaca fascicularis

enzyme has dehydrogenase and reductase activities, enzyme is identical to dihydrodiol dehydrogenase

D-xylono-1,5-lactone + NADPH + H+

-

?

additional information

Macaca fascicularis

D-xylose dehydrogenase catalyzes the NADP+-linked oxidation of dihydrodiols of aromatic hydrocarbons to their corresponding catechols and is regarded as a toxication enzyme in the metabolism of carcinogenic polycyclic aromatic hydrocarbons because oxidation by the enzyme yields reactive and redox-active ortho-quinones and reactive oxygen species, broad substrate specificity indicates additional roles of the enzyme in the metabolism of endogenous and xenobiotic carbonyl compounds and the prevention of the development of glycation

?

-

?

Macaca fascicularis

Q9TQS6

-

the recombinant enzymes are purified from the 12000g supernatants of the homogenates of the cells

Macaca fascicularis

D-xylose + NADP+

enzyme has dehydrogenase and reductase activities, enzyme is identical to dihydrodiol dehydrogenase

684150

Macaca fascicularis

D-xylono-1,5-lactone + NADPH + H+

-

?

additional information

D-xylose dehydrogenase catalyzes the NADP+-linked oxidation of dihydrodiols of aromatic hydrocarbons to their corresponding catechols and is regarded as a toxication enzyme in the metabolism of carcinogenic polycyclic aromatic hydrocarbons because oxidation by the enzyme yields reactive and redox-active ortho-quinones and reactive oxygen species, broad substrate specificity indicates additional roles of the enzyme in the metabolism of endogenous and xenobiotic carbonyl compounds and the prevention of the development of glycation

684150

Macaca fascicularis

?

-

?

dimer

-

Macaca fascicularis

NADP+-dependent D-xylose dehydrogenase

-

Macaca fascicularis

0.4

-

D-xylose

oxidation reaction of mutant R37D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose

Macaca fascicularis

0.45

-

D-xylose

oxidation reaction of mutant A36D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose

Macaca fascicularis

0.5

-

D-xylose

oxidation reaction of mutant H76Q, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose

Macaca fascicularis

0.9

-

D-xylose

oxidation reaction of mutant H79Q, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose

Macaca fascicularis

1

-

D-xylose

oxidation reaction of mutant R41D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose

Macaca fascicularis

1.2

-

D-xylose

oxidation reaction of mutant R37A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose

Macaca fascicularis

1.7

-

D-xylose

oxidation reaction of mutant R41A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose

Macaca fascicularis

2.6

-

D-xylose

oxidation reaction of wild type enzyme, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose

Macaca fascicularis

7.5

-

assay at

Macaca fascicularis

NADP+

-

Macaca fascicularis

wild-type protein and mutants are expressed in Escherichia coli BL21 (DE3)

Macaca fascicularis

NADP+

-

Macaca fascicularis

crystals are obtained at 19.9Â°C in a culture plate via the vapour-diffusion method, crystal structure of dimeric D-xylose dehydrogenase complexed with the inhibitor isoascorbic acid is determined at 2.59 A resolution

Macaca fascicularis

A36D

site-directed mutagenesis

Macaca fascicularis

D280A

by site-directed mutagenesis

Macaca fascicularis

F154A

by site-directed mutagenesis

Macaca fascicularis

F279A

by site-directed mutagenesis

Macaca fascicularis

H76Q

by site-directed mutagenesis

Macaca fascicularis

H79Q

by site-directed mutagenesis

Macaca fascicularis

K97M

by site-directed mutagenesis

Macaca fascicularis

K97R

by site-directed mutagenesis

Macaca fascicularis

R37A

site-directed mutagenesis

Macaca fascicularis

R37D

site-directed mutagenesis

Macaca fascicularis

R41A

by site-directed mutagenesis

Macaca fascicularis

R41D

by site-directed mutagenesis

Macaca fascicularis

W125Y

by site-directed mutagenesis

Macaca fascicularis

W254A

by site-directed mutagenesis

Macaca fascicularis

W254Y

by site-directed mutagenesis

Macaca fascicularis

4-hydroxyacetophenone

-

Macaca fascicularis

isoascorbic acid

competitive inhibitor

Macaca fascicularis

2.4

-

D-xylose

oxidation reaction of mutant R41A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

Macaca fascicularis

2.9

-

D-xylose

oxidation reaction of mutant R41D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

Macaca fascicularis

3.4

-

D-xylose

oxidation reaction of mutant R37A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

Macaca fascicularis

4.4

-

D-xylose

oxidation reaction of wild type enzyme, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

Macaca fascicularis

8

9

D-xylose

oxidation reaction of mutant F279A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

Macaca fascicularis

11

-

D-xylose

oxidation reaction of mutant R37D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

Macaca fascicularis

14

-

D-xylose

oxidation reaction of mutant W254Y, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

Macaca fascicularis

15

-

D-xylose

oxidation reaction of mutant H79Q, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

Macaca fascicularis

36

-

D-xylose

oxidation reaction of mutant F154A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

Macaca fascicularis

41

-

D-xylose

oxidation reaction of mutant H76Q, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

Macaca fascicularis

65

-

D-xylose

oxidation reaction of mutant A36D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

Macaca fascicularis

67

-

D-xylose

oxidation reaction of mutant W125Y, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

Macaca fascicularis

90

-

D-xylose

oxidation reaction of mutant W254A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

Macaca fascicularis

D-xylose + NADP+

Macaca fascicularis

enzyme has dehydrogenase and reductase activities, enzyme is identical to dihydrodiol dehydrogenase

D-xylono-1,5-lactone + NADPH + H+

-

?

additional information

Macaca fascicularis

D-xylose dehydrogenase catalyzes the NADP+-linked oxidation of dihydrodiols of aromatic hydrocarbons to their corresponding catechols and is regarded as a toxication enzyme in the metabolism of carcinogenic polycyclic aromatic hydrocarbons because oxidation by the enzyme yields reactive and redox-active ortho-quinones and reactive oxygen species, broad substrate specificity indicates additional roles of the enzyme in the metabolism of endogenous and xenobiotic carbonyl compounds and the prevention of the development of glycation

?

-

?

the recombinant enzymes are purified from the 12000g supernatants of the homogenates of the cells

Macaca fascicularis

D-xylose + NADP+

enzyme has dehydrogenase and reductase activities, enzyme is identical to dihydrodiol dehydrogenase

684150

Macaca fascicularis

D-xylono-1,5-lactone + NADPH + H+

-

?

additional information

D-xylose dehydrogenase catalyzes the NADP+-linked oxidation of dihydrodiols of aromatic hydrocarbons to their corresponding catechols and is regarded as a toxication enzyme in the metabolism of carcinogenic polycyclic aromatic hydrocarbons because oxidation by the enzyme yields reactive and redox-active ortho-quinones and reactive oxygen species, broad substrate specificity indicates additional roles of the enzyme in the metabolism of endogenous and xenobiotic carbonyl compounds and the prevention of the development of glycation

684150

Macaca fascicularis

?

-

?

dimer

-

Macaca fascicularis

0.4

-

D-xylose

oxidation reaction of mutant R37D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose

Macaca fascicularis

0.45

-

D-xylose

oxidation reaction of mutant A36D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose

Macaca fascicularis

0.5

-

D-xylose

oxidation reaction of mutant H76Q, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose

Macaca fascicularis

0.9

-

D-xylose

oxidation reaction of mutant H79Q, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose

Macaca fascicularis

1

-

D-xylose

oxidation reaction of mutant R41D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose

Macaca fascicularis

1.2

-

D-xylose

oxidation reaction of mutant R37A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose

Macaca fascicularis

1.7

-

D-xylose

oxidation reaction of mutant R41A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose

Macaca fascicularis

2.6

-

D-xylose

oxidation reaction of wild type enzyme, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose

Macaca fascicularis

7.5

-

assay at

Macaca fascicularis

723749

Mihasan

Evidence of a plasmid-encoded ...

Paenarthrobacter nicotinovorans

Res. Microbiol.

164

22-30

2013

-

1

-

3

-

1

-

1

-

3

1

-

3

-

2

-

1

2

-

3

-

1

-

3

1

-

3

-

3

724150

Ahmed

-

Oxidation and reduction of D-x ...

Ogataea angusta

Aust. J. Basic Appl. Sci.

5

95-100

2011

-

2

-

1

-

3

-

2

-

2

-

2

-

3

-

725867

Nygard

Bioconversion of d-xylose to d ...

Trichoderma reesei

Metab. Eng.

13

383-391

2011

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

698940

Johnsen

D-xylose degradation pathway i ...

Haloferax volcanii, Haloferax volcanii DS2

J. Biol. Chem.

284

27290-27303

2009

1

-

2

-

1

-

6

-

3

2

-

3

-

1

-

1

3

-

10

1

2

-

2

-

1

-

2

-

1

-

6

-

3

2

-

1

-

1

3

-

10

1

-

684150

Carbone

Structure of monkey dimeric di ...

Macaca fascicularis

Acta Crystallogr. Sect. D

64

532-542

2008

-

1

15

-

2

13

-

2

-

2

-

1

-

2

1

-

8

1

-

1

-

1

15

-

2

-

13

-

2

-

1

-

2

1

-

8

1

-

686797

Berghaell

Identification in the mould Hy ...

Trichoderma reesei

FEMS Microbiol. Lett.

277

249-253

2007

-

1

-

4

-

1

-

1

-

1

-

1

-

6

1

2

1

-

2

1

-

1

-

1

-

4

-

1

-

1

-

1

-

6

1

-

2

1

-

655884

Johnsen

Novel xylose dehydrogenase in ...

Haloarcula marismortui

J. Bacteriol.

186

6198-6207

2004

-

1

-

3

-

3

2

1

-

1

-

1

-

1

-

5

1

-

2

1

-

3

1

-

2

-

1

2

-

3

-

3

2

1

-

1

-

1

-

5

1

2

1

-

3

1

-

3

286128

Aoki

Identity of dimeric dihydrodio ...

Canis lupus familiaris, Oryctolagus cuniculus, Homo sapiens, Macaca fuscata, Sus scrofa

Chem. Biol. Interact.

130-132

775-784

2001

4

-

2

-

1

4

-

5

-

16

-

4

1

-

8

2

-

18

-

8

-

7

-

4

-

7

-

2

-

1

-

4

-

5

-

4

-

8

2

-

18

-

8

-

286127

Asada

Roles of His-79 and Tyr-180 of ...

Macaca fuscata

Biochem. Biophys. Res. Commun.

278

333-337

2000

1

-

1

-

2

-

1

9

-

2

-

1

-

1

-

1

3

-

3

1

-

1

-

1

-

1

-

2

-

1

-

9

-

2

-

1

-

1

3

-

3

1

-

286126

Zepeda

NADP(+)-dependent D-xylose deh ...

Sus scrofa

Biochem. J.

266

637-644

1990

-

2

9

-

2

-

1

-

1

-

1

-

8

1

-

1

-

7

1

-

2

-

2

-

2

-

9

-

2

-

1

-

1

-

8

1

-

7

1

-

286124

Wissler

-

D-Xylose:NADP+ oxidoreductase ...

Bos taurus, Canis lupus familiaris, Sus scrofa

Hoppe-Seyler's Z. Physiol. Chem.

358

1300-1301

1977

-

3

-

3

-

7

-

24

-

3

-

3

-

3

-

7

-

24

-

286125

Suzuki

Oxidation and reduction of D-x ...

Starmera quercuum

Appl. Microbiol.

25

850-852

1973

-

1

-

1

-

1

-

1

-

1

-

Structure of monkey dimeric dihydrodiol dehydrogenase in complex with isoascorbic acid

Data extracted from this reference: