Crystallization (Comment) | Organism |
---|---|
X-ray diffraction structure determination and analysis of enzyme mutant A12K/D35S/V36R complex with NADP+ | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
A12K/D35S/V36R | site-directed mutagenesis, the triple mutant has a value of 570000 M/s in reaction with NADP+, higher than that of the wild-type IDH with NAD+. The binding of the coenzyme in the mutant is altered such that although the nicotinamide ring maintains the required position for catalysis, the coenzyme has twisted by nearly 90°, so the adenine moiety no longer binds to a hydrophobic cleft in the Rossmann fold as in the wild-type enzyme | Bacillus subtilis |
D35S/V36R | site-directed mutagenesis, the double mutant prefers NADP+ to NAD+ by a factor of 5. The mutant is an excellent catalyst with a second-order rate constant with respect to NADP of 370000 M/s | Bacillus subtilis |
additional information | convertion of NAD+-specific inositol dehydrogenase to an efficient NADP+-selective catalyst to enhance understanding of coenzyme selectivity and to create an enzyme capable of recycling NADP+ in biocatalytic processes | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol + NAD+ | Bacillus subtilis | - |
2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+ | i.e. scyllo-inosose | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P26935 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol + NAD+ | - |
Bacillus subtilis | 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+ | i.e. scyllo-inosose | ? |
Synonyms | Comment | Organism |
---|---|---|
IDH | - |
Bacillus subtilis |
myo-inositol dehydrogenase | - |
Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | strictly dependent on | Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is related to the GFO/MocA/IDH family of dehydrogenases | Bacillus subtilis |