Inhibitors | Comment | Organism | Structure |
---|---|---|---|
FMN | 30% inhibition at 0.1 mM | Oryctolagus cuniculus | |
Quinacrine | slight inhibition at 1 mM | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
cholest-5-ene-3beta,7alpha-diol | - |
Oryctolagus cuniculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
gel filtration | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholest-5-ene-3beta,7alpha-diol + NAD+ | Oryctolagus cuniculus | highly specific | 7alpha-hydroxycholest-4-en-3-one + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
rabbit | - |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.23 | - |
- |
Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholest-5-ene-3beta,7alpha-diol + NAD+ | highly specific | Oryctolagus cuniculus | 7alpha-hydroxycholest-4-en-3-one + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 46000, SDS-PAGE | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | in potassium phosphate buffer | Oryctolagus cuniculus |
9 | - |
in Tris-HCl buffer | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | can not be replaced by NADP+ | Oryctolagus cuniculus |