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Literature summary for 1.1.1.181 extracted from

  • Wikvall, K.
    Purification and properties of a 3beta-hydroxy-DELTA5-C27-steroid oxidoreductase from rabbit liver microsomes (1981), J. Biol. Chem., 256, 3376-3380.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
FMN 30% inhibition at 0.1 mM Oryctolagus cuniculus
Quinacrine slight inhibition at 1 mM Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
cholest-5-ene-3beta,7alpha-diol
-
Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
gel filtration Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholest-5-ene-3beta,7alpha-diol + NAD+ Oryctolagus cuniculus highly specific 7alpha-hydroxycholest-4-en-3-one + NADH
-
r

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
rabbit
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
0.23
-
-
Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholest-5-ene-3beta,7alpha-diol + NAD+ highly specific Oryctolagus cuniculus 7alpha-hydroxycholest-4-en-3-one + NADH
-
r

Subunits

Subunits Comment Organism
monomer 1 * 46000, SDS-PAGE Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 in potassium phosphate buffer Oryctolagus cuniculus
9
-
in Tris-HCl buffer Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
NAD+ can not be replaced by NADP+ Oryctolagus cuniculus