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Literature summary for 1.1.1.183 extracted from

  • Sato-Masumoto, N.; Ito, M.
    Two types of alcohol dehydrogenase from Perilla can form citral and perillaldehyde (2014), Phytochemistry, 104, 12-20.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination, comparison, and analysis, semi-quantitative RT-PCR enzyme expression analysis Perilla frutescens
DNA and amino acid sequence determination, comparison, and analysis, semi-quantitative RT-PCR enzyme expression analysis Perilla frutescens var. hirtella
DNA and amino acid sequence determination, comparison, and analysis, semi-quantitative RT-PCR enzyme expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS Perilla frutescens var. hirtella

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geraniol + NADP+ Perilla frutescens
-
geranial + NADPH + H+
-
?
geraniol + NADP+ Perilla frutescens var. hirtella
-
geranial + NADPH + H+
-
?
geraniol + NADP+ Perilla frutescens var. hirtella
-
geranial + NADPH + H+
-
r
geraniol + NADP+ Perilla frutescens 32
-
geranial + NADPH + H+
-
?
geraniol + NADP+ Perilla frutescens var. hirtella 5031
-
geranial + NADPH + H+
-
?
geraniol + NADP+ Perilla frutescens var. hirtella 87
-
geranial + NADPH + H+
-
?
geraniol + NADP+ Perilla frutescens var. hirtella 87
-
geranial + NADPH + H+
-
r
additional information Perilla frutescens var. hirtella the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products ?
-
?
additional information Perilla frutescens the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products. Oil component identification by gas mass spectrometry ?
-
?
additional information Perilla frutescens 32 the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products. Oil component identification by gas mass spectrometry ?
-
?
additional information Perilla frutescens var. hirtella 87 the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products ?
-
?

Organism

Organism UniProt Comment Textmining
Perilla frutescens K9S1J6
-
-
Perilla frutescens K9S1J6 PA type
-
Perilla frutescens 32 K9S1J6 PA type
-
Perilla frutescens var. hirtella
-
-
-
Perilla frutescens var. hirtella K9RZL7
-
-
Perilla frutescens var. hirtella K9RZL7 C type
-
Perilla frutescens var. hirtella K9S0X0
-
-
Perilla frutescens var. hirtella K9S0X0 SF type
-
Perilla frutescens var. hirtella 5031 K9S0X0 SF type
-
Perilla frutescens var. hirtella 87 K9RZL7 C type
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS Perilla frutescens var. hirtella

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Perilla frutescens
-
leaf
-
Perilla frutescens var. hirtella
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranial + NADPH + H+
-
Perilla frutescens var. hirtella geraniol + NADP+
-
r
geranial + NADPH + H+
-
Perilla frutescens var. hirtella 87 geraniol + NADP+
-
r
geranial + NADPH + H+
-
Perilla frutescens var. hirtella nerol + NADP+ PcGeDH can reduce citral to geraniol and nerol when NADPH is added as cofactor, but not when NADP+ is the cofactor. PcGeDH can catalyze either oxidation or reduction. Oil component identification by gas mass spectrometry r
geranial + NADPH + H+
-
Perilla frutescens var. hirtella 87 nerol + NADP+ PcGeDH can reduce citral to geraniol and nerol when NADPH is added as cofactor, but not when NADP+ is the cofactor. PcGeDH can catalyze either oxidation or reduction. Oil component identification by gas mass spectrometry r
geraniol + NADP+
-
Perilla frutescens geranial + NADPH + H+
-
?
geraniol + NADP+
-
Perilla frutescens var. hirtella geranial + NADPH + H+
-
?
geraniol + NADP+
-
Perilla frutescens var. hirtella geranial + NADPH + H+
-
r
geraniol + NADP+
-
Perilla frutescens 32 geranial + NADPH + H+
-
?
geraniol + NADP+
-
Perilla frutescens var. hirtella 5031 geranial + NADPH + H+
-
?
geraniol + NADP+
-
Perilla frutescens var. hirtella 87 geranial + NADPH + H+
-
?
geraniol + NADP+
-
Perilla frutescens var. hirtella 87 geranial + NADPH + H+
-
r
additional information the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products Perilla frutescens var. hirtella ?
-
?
additional information the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products. Oil component identification by gas mass spectrometry Perilla frutescens ?
-
?
additional information the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products. Oil component identification by gas mass spectrometry Perilla frutescens var. hirtella ?
-
?
additional information the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products. Oil component identification by gas mass spectrometry Perilla frutescens 32 ?
-
?
additional information the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products. Oil component identification by gas mass spectrometry Perilla frutescens var. hirtella 5031 ?
-
?
additional information the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products Perilla frutescens var. hirtella 87 ?
-
?

Synonyms

Synonyms Comment Organism
GeDH
-
Perilla frutescens
GeDH
-
Perilla frutescens var. hirtella

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
30
-
assay at Perilla frutescens
30
-
assay at Perilla frutescens var. hirtella

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Perilla frutescens
7
-
assay at Perilla frutescens var. hirtella

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Perilla frutescens
NADP+
-
Perilla frutescens var. hirtella

General Information

General Information Comment Organism
evolution GeDH gene expression in C-, PA-, PK-, and PP-type Perilla Perilla frutescens
evolution GeDH gene expression in C-, PA-, PK-, and PP-type Perilla Perilla frutescens var. hirtella
metabolism the enzyme geraniol dehydrogenase is involved in the biosynthetic pathway from geranyl diphosphate to citral, the main compound of citral-type perilla essential oil Perilla frutescens
metabolism the enzyme geraniol dehydrogenase is involved in the biosynthetic pathway from geranyl diphosphate to citral, the main compound of citral-type perilla essential oil Perilla frutescens var. hirtella
metabolism the enzyme geraniol dehydrogenase is involved in the biosynthetic pathway from geranyl diphosphate to citral, the main compound of citral-type perilla essential oil. Geranial is formed from geraniol by enzymatic reaction of Perilla aldo-keto reductase or Perilla GeDH Perilla frutescens var. hirtella
physiological function two types of alcohol dehydrogenases, an aldo-keto reductase (AKR) and a geraniol dehydrogenase (GeDH), are thought to participate in the biosynthesis of perilla essential oil components, such as citral and perillaldehyde Perilla frutescens
physiological function two types of alcohol dehydrogenases, an aldo-keto reductase (AKR) and a geraniol dehydrogenase (GeDH), are thought to participate in the biosynthesis of perilla essential oil components, such as citral and perillaldehyde Perilla frutescens var. hirtella