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Literature summary for 1.1.1.184 extracted from

  • Wada, M.; Kataoka, M.; Kawabata, H.; Yasohara, Y.; Kizaki, N.; Hasegawa, J.; Shimizu, S.
    Purification and characterization of NADPH-dependent carbonyl reductase, involved in stereoselective reduction of ethyl 4-chloro-3-oxobutanoate, from Candida magnoliae (1998), Biosci. Biotechnol. Biochem., 62, 280-285.
    View publication on PubMed
Search for more literature for 1.1.1.184

Inhibitors

Inhibitors Comment Organism Structure
Hg2+
-
 Starmerella magnoliae
quercetin
-
 Starmerella magnoliae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 NADPH
-
 Starmerella magnoliae
4.1
-
 ethyl 2-chloro-3-oxobutanoate
-
 Starmerella magnoliae
4.6
-
 ethyl 4-chloro-3-oxobutanoate
-
 Starmerella magnoliae
7.4
-
 diacetyl
-
 Starmerella magnoliae
10
-
 ketopantoyl lactone
-
 Starmerella magnoliae
10
-
 methyl 4-chloro-3-oxobutanoate
-
 Starmerella magnoliae
21
-
 ethyl pyruvate
-
 Starmerella magnoliae
33
-
 2,3-Pentanedione
-
 Starmerella magnoliae
39
-
 methyl 2-chloro-3-oxobutanoate
-
 Starmerella magnoliae
61
-
 ethyl 2-oxobutanoate
-
 Starmerella magnoliae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 Edman degradation Starmerella magnoliae
32000
-
 2 * 32000, SDS-PAGE Starmerella magnoliae
76000
-
 gel filtration Starmerella magnoliae

Organism

Organism UniProt Comment Textmining
Starmerella magnoliae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Starmerella magnoliae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-pentanedione + NADPH + H+
-
 Starmerella magnoliae ? + NADP+
-
 ?
diacetyl + NADPH + H+
-
 Starmerella magnoliae acetoin + NADP+
-
 r
ethyl 2-chloro-3-oxobutanoate + NADPH + H+
-
 Starmerella magnoliae ethyl-2-chloro-3-hydroxybutanoate + NADP+
-
 ?
ethyl 2-oxobutanoate + NADPH + H+ 16% activity compared to ethyl 4-chloro-3-oxobutanoate Starmerella magnoliae ethyl 2-hydroxybutanoate + NADP+
-
 r
ethyl 3-oxobutanoate + NADPH
-
 Starmerella magnoliae ethyl 3-hydroxybutanoate + NADP+
-
 ?
ethyl 4-chloro-3-oxobutanoate + NADPH
-
 Starmerella magnoliae ethyl-4-chloro-3-hydroxybutanoate + NADP+
-
 ?
ethyl pyruvate + NADPH + H+ 24% activity compared to ethyl 4-chloro-3-oxobutanoate Starmerella magnoliae ethyl 2-hydroxypropanoic acid + NADP+
-
 r
ketopantoyl lactone + NADPH
-
 Starmerella magnoliae ?
-
 ?
methyl 2-chloro-3-oxobutanoate + NADPH + H+
-
 Starmerella magnoliae (S)-methyl 2-chloro-3-hydroxybutanoate + NADP+
-
 ?
methyl 4-chloro-3-oxobutanoate + NADPH + H+ 9% activity compared to ethyl 4-chloro-3-oxobutanoate Starmerella magnoliae methyl 4-chloro-3-hydroxybutanoate + NADP+
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 32000, SDS-PAGE Starmerella magnoliae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 pH 7.0, reduction of ethyl 4-chloro-3-oxobutanoate Starmerella magnoliae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Starmerella magnoliae

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Starmerella magnoliae