Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3,4-trinitrobenzenesulfonate sodium | acts on a catalytic lysine located at the cofactor binding site, rapid inactivation of the liver enzyme, kinetics, NADP+, 2'-AMP, 2',5'-ADP, and 2'-phospho-5'-ADP-ribose partly protect, but 4-acetylpyridine, 5'-AMP, 5'-ADP, NMN and NAD+ do not | Oryctolagus cuniculus | |
additional information | protective effects of cofactor derivatives against inactivation by phenylglyoxal and 2,3,4-trinitrobenzenesulfonate sodium, overview | Oryctolagus cuniculus | |
Phenylglyoxal | acts on a catalytic arginine located at the cofactor binding site, rapid inactivation of the liver enzyme, kinetics, NADP+, 2'-AMP, 2',5'-ADP, and 2'-phospho-5'-ADP-ribose partly protect, but 4-acetylpyridine, 5'-AMP, 5'-ADP, NMN and NAD+ do not | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
loxoprofen | pH 6.5, 30°C | Oryctolagus cuniculus | |
0.92 | - |
acetohexamide | pH 6.5, 30°C | Oryctolagus cuniculus | |
1.44 | - |
befunolol | pH 6.5, 30°C | Oryctolagus cuniculus | |
3 | - |
daunorubicin | pH 6.5, 30°C | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Oryctolagus cuniculus | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
R-CHOH-R' + NADP+ = R-CO-R' + NADPH + H+ | ordered bi bi mechanism, in which NADPH binds first and NADP+ leaves last | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Oryctolagus cuniculus | - |
liver | - |
Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-acetylpyridine + NADPH | - |
Oryctolagus cuniculus | ? | - |
? | |
acetohexamide + NADPH | - |
Oryctolagus cuniculus | ? | - |
? | |
befunolol + NADPH | - |
Oryctolagus cuniculus | ? | - |
? | |
daunorubicin + NADPH | - |
Oryctolagus cuniculus | ? | - |
? | |
loxoprofen + NADPH | - |
Oryctolagus cuniculus | ? | - |
? | |
additional information | substrate specificity | Oryctolagus cuniculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
carbonyl reductase | - |
Oryctolagus cuniculus |
CR | - |
Oryctolagus cuniculus |
RLCR | - |
Oryctolagus cuniculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | catalytically important arginine and lysine residues are located in the enzymes' cofactor binding site and interact with the 2'-phosphate group of the cofactor | Oryctolagus cuniculus |