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Literature summary for 1.1.1.184 extracted from

  • Zhang, R.; Xu, Y.; Geng, Y.; Wang, S.; Sun, Y.; Xiao, R.
    Improved production of (R)-1-phenyl-1,2-ethanediol by a codon-optimized R-specific carbonyl reductase from Candida parapsilosis in Escherichia coli (2009), Appl. Biochem. Biotechnol., 160, 868-878.
    View publication on PubMed
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Application

Application Comment Organism
industry improved (R)-1-phenyl-1,2-ethanediol production by a codon optimized R-specific carbonyl reductase whose coding gene rcr is engineered by truncating its disorder sequence of 4-27 bp at 5'-terminus and adaption of codon usage bias in Escherichia coli. It will provide a new method to establish an effective expression system for improving chiral alcohol productivity by codon optimization Candida parapsilosis

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant enzymes inserted into vector pQE30a and expressed in Escherichia coli BL21 (AITM) cells Candida parapsilosis

Protein Variants

Protein Variants Comment Organism
additional information codon variants DELTA24 (truncation of disorder sequence of 4-27 bp at the 5'-terminus), aRCR (its nine out of 11 rare codons are adapted according to the codon bias in Escherichia coli), and mRCR (its disorder sequence of 4-27 bp at the 5'-terminus is truncated and nine out of 11 rare codons are adapted) are expressed at higher levels than the wild-type. Mutant mRCR presents highest enzyme activity. When compared with wild-type, mRCR shows an increase of 35.6% in the total activity of cell-free extracts Candida parapsilosis

Organism

Organism UniProt Comment Textmining
Candida parapsilosis A1X808
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-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes purified to homogeneity by Ni-affinity chromatography Candida parapsilosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxyacetophenone + NADPH + H+
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 Candida parapsilosis (R)-1-phenyl-1,2-ethanediol + NADP+
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 ?

Synonyms

Synonyms Comment Organism
R-specific carbonyl reductase
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 Candida parapsilosis