Literature summary for 1.1.1.184 extracted from

Ye, Q.; Yan, M.; Xu, L.; Cao, H.; Li, Z.; Chen, Y.; Li, S.; Ying, H.
A novel carbonyl reductase from Pichia stipitis for the production of ethyl (S)-4-chloro-3-hydroxybutanoate (2009), Biotechnol. Lett., 31, 537-542.

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Application

Application	Comment	Organism
industry	enzyme has a promising future for industrial application	Scheffersomyces stipitis

Cloned(Commentary)

Cloned (Comment)	Organism
expressed from vector pET-22b in Escherichia coli BL21 (DE3)	Scheffersomyces stipitis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30100	-	sequence analysis	Scheffersomyces stipitis

Organism

Organism	UniProt	Comment	Textmining
Scheffersomyces stipitis	A3GF07	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-chloro-3-oxobutanoate + NADPH + H+	reduction of 4-chloro-3-oxobutanoate to ethyl (S)-4-chloro-3-hydroxybutanoate by transformants in an aqueous mono-phase system for 18 h, gives a molar yield of 94% and an optical purity of the (S)-isomer of more than 99% enantiomeric excess	Scheffersomyces stipitis	ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
carbonyl reductase	-	Scheffersomyces stipitis

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Release 2023.1 (January 2023)