Organism | UniProt | Comment | Textmining |
---|---|---|---|
Komagataella pastoris | - |
- |
- |
Komagataella pastoris GS115 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.073 | - |
substrate 1-phenylethanonee, pH 6.5, temperature not specified in the publication | Komagataella pastoris |
0.074 | - |
substrate 1-(4-chlorophenyl)ethanone, pH 6.5, temperature not specified in the publication | Komagataella pastoris |
0.522 | - |
substrate ethyl 3-oxopentanoate, pH 6.5, temperature not specified in the publication | Komagataella pastoris |
6.3 | - |
substrate 4-chlorobenzaldehyde, pH 6.5, temperature not specified in the publication | Komagataella pastoris |
7 | - |
substrate 4-fluorobenzaldehyde, pH 6.5, temperature not specified in the publication | Komagataella pastoris |
10.9 | - |
substrate 4-bromobenzaldehyde, pH 6.5, temperature not specified in the publication | Komagataella pastoris |
1502 | - |
substrate ethyl 4-chloro-3-oxobutanoate, pH 6.5, temperature not specified in the publication | Komagataella pastoris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(4-chlorophenyl)ethanone + NADPH + H+ | - |
Komagataella pastoris | (S)-1-(4-chlorophenyl)ethanol + NADP+ | 86% conversion, 99% enantiomeric excess | ? | |
1-(4-chlorophenyl)ethanone + NADPH + H+ | - |
Komagataella pastoris GS115 | (S)-1-(4-chlorophenyl)ethanol + NADP+ | 86% conversion, 99% enantiomeric excess | ? | |
1-phenylethanone + NADPH + H+ | - |
Komagataella pastoris | (S)-1-phenylethanol + NADP+ | 60% conversion, 99% enantiomeric excess | ? | |
1-phenylethanone + NADPH + H+ | - |
Komagataella pastoris GS115 | (S)-1-phenylethanol + NADP+ | 60% conversion, 99% enantiomeric excess | ? | |
4-bromobenzaldehyde + NADPH + H+ | 99% conversion | Komagataella pastoris | (4-bromophenyl)methanol + NADP+ | - |
? | |
4-bromobenzaldehyde + NADPH + H+ | 99% conversion | Komagataella pastoris GS115 | (4-bromophenyl)methanol + NADP+ | - |
? | |
4-chlorobenzaldehyde + NADPH + H+ | 99% conversion | Komagataella pastoris | (4-chlorophenyl)methanol + NADP+ | - |
? | |
4-chlorobenzaldehyde + NADPH + H+ | 99% conversion | Komagataella pastoris GS115 | (4-chlorophenyl)methanol + NADP+ | - |
? | |
4-fluorobenzaldehyde + NADPH + H+ | 99% conversion | Komagataella pastoris | (4-fluorophenyl)methanol + NADP+ | - |
? | |
ethyl 3-oxopentanoate + NADPH + H+ | - |
Komagataella pastoris | ethyl (S)-3-hydroxypentanoate + NADP+ | 99% conversion, 99% enantiomeric excess | ? | |
ethyl 4-chloro-3-oxobutanoate + NADPH + H+ | 99% conversion, 92% enantiomeric excess | Komagataella pastoris | ethyl (R)-4-chloro-3-hydroxybutanoate + NADP+ | 99% conversion, 92% enantiomeric excess | ? | |
additional information | enzyme is able to reduce both aldehydes and ketones | Komagataella pastoris | ? | - |
? | |
additional information | enzyme is able to reduce both aldehydes and ketones | Komagataella pastoris GS115 | ? | - |
? | |
phenylglyoxal + 2 NADPH + 2 H+ | - |
Komagataella pastoris | (R)-1-phenyl-1,2-ethanediol + 2 NADP+ | 99% yield and 99% enantiomeric excess, by coupling with D-glucose dehydrogenase for the regeneration of cofactor NADPH | ? |