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Literature summary for 1.1.1.185 extracted from

  • Bernardo, A.; Burgos, J.; Martin, R.
    Purification and some properties of L-glycol dehydrogenase from hens muscle (1981), Biochim. Biophys. Acta, 659, 189-198.
    View publication on PubMed
Search for more literature for 1.1.1.185

General Stability

General Stability Organism
easily inactivated in high molarity buffers Gallus gallus
very stable in both water and low molarity buffers of pH 7 at 0-4°C Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Gallus gallus
0.026
-
 glyceraldehyde pI 7.2 form Gallus gallus
0.026
-
 glyceraldehyde enzyme form of pI 7.2, at pH 7.0 and 25°C Gallus gallus
0.029
-
 glyceraldehyde pI 6.2 form Gallus gallus
0.029
-
 glyceraldehyde enzyme form of pI 6.2, at pH 7.0 and 25°C Gallus gallus
0.053
-
 Glyoxal pI 6.2 form Gallus gallus
0.053
-
 Glyoxal enzyme form of pI 6.2, at pH 7.0 and 25°C Gallus gallus
0.06
-
 2,3-Pentanedione pI 7.2 form Gallus gallus
0.097
-
 2,3-Pentanedione pI 6.2 form Gallus gallus
0.098
-
 methylglyoxal pI 6.2 form Gallus gallus
0.102
-
 methylglyoxal pI 7.2 form Gallus gallus
0.109
-
 diacetyl pI 7.2 form Gallus gallus
0.38
-
 diacetyl pI 6.2 form Gallus gallus
0.411
-
 Glyoxal pI 4.8 form Gallus gallus
0.411
-
 Glyoxal enzyme form of pI 4.8, at pH 7.0 and 25°C Gallus gallus
0.522
-
 Glyoxal pI 7.2 form Gallus gallus
0.522
-
 Glyoxal enzyme form of pI 7.2, at pH 7.0 and 25°C Gallus gallus
0.566
-
 glyceraldehyde pI 4.8 form Gallus gallus
0.566
-
 glyceraldehyde enzyme form of pI 4.8, at pH 7.0 and 25°C Gallus gallus
0.675
-
 2,3-Pentanedione pI 4.8 form Gallus gallus
2.013
-
 acetoin pI 6.2 form Gallus gallus
2.164
-
 acetoin pI 4.8 form Gallus gallus
2.59
-
 acetoin pI 7.2 form Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 gel filtration Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
 hen
-

Purification (Commentary)

Purification (Comment) Organism
3 enzyme forms: pI 7.2, pI 6.2 and pI 4.8 Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Gallus gallus

Storage Stability

Storage Stability Organism
-18°C, enzyme at any stage of purification Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-pentanedione + NADH
-
 Gallus gallus L-acetylethylcarbinol + NAD+
-
 ?
diacetyl + NADH
-
 Gallus gallus L-acetoin + NAD+
-
 ?
ethyl pyruvate + NAD(P)H
-
 Gallus gallus ethyl lactate
-
 ?
glyceraldehyde + NADPH + H+
-
 Gallus gallus ?
-
 r
glyoxal + NADPH + H+
-
 Gallus gallus ?
-
 r
methyl pyruvate + NAD(P)H
-
 Gallus gallus ?
-
 ?
methylglyoxal + NADH
-
 Gallus gallus L-lactaldehyde
-
 ?
additional information not: acetaldehyde, monoketones, non-vicinal diketones, free ketoacids Gallus gallus ?
-
 ?
R1-CO-CHOH-R2 + NAD(P)H 2-hydroxycarbonyl compound e.g. glycolaldehyde, glyceraldehyde, acetoin, acetylethylcarbinol, diacetylmethylcarbinol Gallus gallus R1-CHOH-CHOH-R2 + NAD(P)+
-
 r
R1-CO-CHOH-R2 + NAD(P)H much more efficient in dehydrogenase direction Gallus gallus R1-CHOH-CHOH-R2 + NAD(P)+
-
 r
R1-CO-CO-R2 + NAD(P)H methylglyoxal, diacetyl, 2,3-pentanedione Gallus gallus R1-CO-CHOH-R2 + NAD(P)+
-
 ?
R1-CO-CO-R2 + NAD(P)H vicinal dicarbonyl: e.g. glyoxal Gallus gallus R1-CO-CHOH-R2 + NAD(P)+
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6.6
-
 Gallus gallus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 very stable in both water and low molarity buffers of pH 7 at 0-4°C Gallus gallus

Cofactor

Cofactor Comment Organism Structure
additional information no activity with alpha-NADH Gallus gallus
NAD+
-
 Gallus gallus
NADH
-
 Gallus gallus
NADPH
-
 Gallus gallus