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Literature summary for 1.1.1.185 extracted from

  • Bernardo, A.; Burgos, J.; Martin, R.
    Purification and some properties of L-glycol dehydrogenase from hens muscle (1981), Biochim. Biophys. Acta, 659, 189-198.
    View publication on PubMed

General Stability

General Stability Organism
easily inactivated in high molarity buffers Gallus gallus
very stable in both water and low molarity buffers of pH 7 at 0-4┬░C Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Gallus gallus
0.026
-
glyceraldehyde pI 7.2 form Gallus gallus
0.026
-
glyceraldehyde enzyme form of pI 7.2, at pH 7.0 and 25┬░C Gallus gallus
0.029
-
glyceraldehyde pI 6.2 form Gallus gallus
0.029
-
glyceraldehyde enzyme form of pI 6.2, at pH 7.0 and 25┬░C Gallus gallus
0.053
-
Glyoxal pI 6.2 form Gallus gallus
0.053
-
Glyoxal enzyme form of pI 6.2, at pH 7.0 and 25┬░C Gallus gallus
0.06
-
2,3-Pentanedione pI 7.2 form Gallus gallus
0.097
-
2,3-Pentanedione pI 6.2 form Gallus gallus
0.098
-
methylglyoxal pI 6.2 form Gallus gallus
0.102
-
methylglyoxal pI 7.2 form Gallus gallus
0.109
-
diacetyl pI 7.2 form Gallus gallus
0.38
-
diacetyl pI 6.2 form Gallus gallus
0.411
-
Glyoxal pI 4.8 form Gallus gallus
0.411
-
Glyoxal enzyme form of pI 4.8, at pH 7.0 and 25┬░C Gallus gallus
0.522
-
Glyoxal pI 7.2 form Gallus gallus
0.522
-
Glyoxal enzyme form of pI 7.2, at pH 7.0 and 25┬░C Gallus gallus
0.566
-
glyceraldehyde pI 4.8 form Gallus gallus
0.566
-
glyceraldehyde enzyme form of pI 4.8, at pH 7.0 and 25┬░C Gallus gallus
0.675
-
2,3-Pentanedione pI 4.8 form Gallus gallus
2.013
-
acetoin pI 6.2 form Gallus gallus
2.164
-
acetoin pI 4.8 form Gallus gallus
2.59
-
acetoin pI 7.2 form Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
gel filtration Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
hen
-

Purification (Commentary)

Purification (Comment) Organism
3 enzyme forms: pI 7.2, pI 6.2 and pI 4.8 Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
additional information
-
-
Gallus gallus

Storage Stability

Storage Stability Organism
-18┬░C, enzyme at any stage of purification Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-pentanedione + NADH
-
Gallus gallus L-acetylethylcarbinol + NAD+
-
?
diacetyl + NADH
-
Gallus gallus L-acetoin + NAD+
-
?
ethyl pyruvate + NAD(P)H
-
Gallus gallus ethyl lactate
-
?
glyceraldehyde + NADPH + H+
-
Gallus gallus ?
-
r
glyoxal + NADPH + H+
-
Gallus gallus ?
-
r
methyl pyruvate + NAD(P)H
-
Gallus gallus ?
-
?
methylglyoxal + NADH
-
Gallus gallus L-lactaldehyde
-
?
additional information not: acetaldehyde, monoketones, non-vicinal diketones, free ketoacids Gallus gallus ?
-
?
R1-CO-CHOH-R2 + NAD(P)H 2-hydroxycarbonyl compound e.g. glycolaldehyde, glyceraldehyde, acetoin, acetylethylcarbinol, diacetylmethylcarbinol Gallus gallus R1-CHOH-CHOH-R2 + NAD(P)+
-
r
R1-CO-CHOH-R2 + NAD(P)H much more efficient in dehydrogenase direction Gallus gallus R1-CHOH-CHOH-R2 + NAD(P)+
-
r
R1-CO-CO-R2 + NAD(P)H methylglyoxal, diacetyl, 2,3-pentanedione Gallus gallus R1-CO-CHOH-R2 + NAD(P)+
-
?
R1-CO-CO-R2 + NAD(P)H vicinal dicarbonyl: e.g. glyoxal Gallus gallus R1-CO-CHOH-R2 + NAD(P)+
-
?

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
25
-
assay at Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6.6
-
Gallus gallus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
very stable in both water and low molarity buffers of pH 7 at 0-4┬░C Gallus gallus

Cofactor

Cofactor Comment Organism Structure
additional information no activity with alpha-NADH Gallus gallus
NAD+
-
Gallus gallus
NADH
-
Gallus gallus
NADPH
-
Gallus gallus