General Stability | Organism |
---|---|
easily inactivated in high molarity buffers | Gallus gallus |
very stable in both water and low molarity buffers of pH 7 at 0-4°C | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetone | pI 7.2 form | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
hen | - |
Purification (Comment) | Organism |
---|---|
3 enzyme forms: pI 7.2, pI 6.2 and pI 4.8 | Gallus gallus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an L-glycol + NAD(P)+ = a 2-hydroxycarbonyl compound + NAD(P)H + H+ | glyoxal reduction by enzyme form pI 7.2 follows ordered bi-bi mechanism in which the coenzyme is the first substrate to bind to the enzyme | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.4 | - |
- |
Gallus gallus |
Storage Stability | Organism |
---|---|
-18°C, enzyme at any stage of purification | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
R1-CO-CHOH-R2 + NAD(P)H | - |
Gallus gallus | R1-CHOH-CHOH-R2 + NAD(P)+ | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6.6 | - |
Gallus gallus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
very stable in both water and low molarity buffers of pH 7 at 0-4°C | Gallus gallus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Gallus gallus | |
NADH | - |
Gallus gallus | |
NADPH | - |
Gallus gallus |