Cloned (Comment) | Organism |
---|---|
liver-type isozyme, full length cDNA from cDNA library, DNA and amino acid sequence determination and analysis, transient expression of wild-type and mutant enzymes in COS-7 cells | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
H170C | site-directed mutagenesis, increased Km for prostaglandin PGD2 and 8fold decreased Km for prostaglandin PGH2 compared to the wild-type enzyme | Bos taurus |
K225S | site-directed mutagenesis, slightly increased Km for prostaglandin PGD2 and PGH2 compared to the wild-type enzyme | Bos taurus |
N306Y | site-directed mutagenesis, increased Km for prostaglandin PGD2 and 5fold increased activity with prostaglandin PGH2 compared to the wild-type enzyme | Bos taurus |
R223L | site-directed mutagenesis, increased Km for prostaglandin PGD2 compared to the wild-type enzyme | Bos taurus |
R27E | site-directed mutagenesis, increased Km for prostaglandin PGD2 compared to the wild-type enzyme | Bos taurus |
R91Q | site-directed mutagenesis, increased Km for prostaglandin PGD2 compared to the wild-type enzyme | Bos taurus |
S301R | site-directed mutagenesis, slightly increased Km for prostaglandin PGD2 and PGH2 compared to the wild-type enzyme | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
9,10-phenanthrenequinone | recombinant wild-type liver-type isozyme, 37°C | Bos taurus | |
0.003 | - |
prostaglandin PGH2 | recombinant liver-type isozyme, mutant H170C, 37°C | Bos taurus | |
0.01 | - |
prostaglandin PGH2 | recombinant liver-type isozyme, mutants R91Q, R223L and N306Y, 37°C | Bos taurus | |
0.014 | - |
prostaglandin PGH2 | recombinant liver-type isozyme, mutant R27E, 37°C | Bos taurus | |
0.015 | - |
prostaglandin PGD2 | recombinant wild-type liver-type isozyme, 37°C | Bos taurus | |
0.025 | - |
prostaglandin PGH2 | recombinant wild-type liver-type isozyme, 37°C | Bos taurus | |
0.028 | - |
prostaglandin PGH2 | recombinant liver-type isozyme, mutant K225S, 37°C | Bos taurus | |
0.03 | - |
prostaglandin PGH2 | recombinant liver-type isozyme, mutant S301R, 37°C | Bos taurus | |
0.04 | - |
prostaglandin PGD2 | recombinant liver-type isozyme mutant S301R, 37°C | Bos taurus | |
0.05 | - |
prostaglandin PGD2 | recombinant liver-type isozyme mutant K225S, 37°C | Bos taurus | |
0.075 | - |
prostaglandin PGD2 | recombinant liver-type isozyme mutant H170C, 37°C | Bos taurus | |
0.1 | - |
prostaglandin PGD2 | recombinant liver-type isozyme mutant N306Y, 37°C | Bos taurus | |
0.11 | - |
prostaglandin PGD2 | recombinant liver-type isozyme mutant R27E, 37°C | Bos taurus | |
0.12 | - |
prostaglandin PGD2 | recombinant wild-type lung-type isozyme | Bos taurus | |
0.145 | - |
prostaglandin PGD2 | recombinant liver-type isozyme mutant R91Q, 37°C | Bos taurus | |
0.18 | - |
prostaglandin PGD2 | recombinant liver-type isozyme mutant R223L, 37°C | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | liver-type isozyme | Bos taurus | 5737 | - |
additional information | no liver-type isozyme in mitochondrion, nucleus, or Golgi apparatus | Bos taurus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36666 | - |
1 * 36666, lung-type isozyme, sequence calculation, 1 * 36742, liver-type isozyme, sequence calculation | Bos taurus |
36670 | - |
lung-type isozyme, sequence calculation | Bos taurus |
36740 | - |
liver-type isozyme, sequence calculation | Bos taurus |
36742 | - |
1 * 36666, lung-type isozyme, sequence calculation, 1 * 36742, liver-type isozyme, sequence calculation | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
liver- and lung-type isozymes | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type liver-type isoyzme and mutant enzymes from COS-7 cells, to homogeneity | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endothelium | liver-type isozyme, sinusoidal endothelial cells in the liver | Bos taurus | - |
liver | liver-type isozyme, sinusoidal endothelial cells | Bos taurus | - |
lung | lung-type isozyme | Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
9,10-phenanthrenequinone + NADPH | - |
Bos taurus | ? | - |
? | |
prostaglandin D2 + NADPH + H+ | amino acid residues Arg27, Arg91, His170, Arg223, and Asn306 are involved in substrate binding | Bos taurus | prostaglandin 9alpha,11beta-F2 + NADP+ | - |
? | |
prostaglandin H2 + NADPH + H+ | His170 is involved in binding, and Asn306 is critical for catalytic activity | Bos taurus | prostaglandin F2alpha + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 36666, lung-type isozyme, sequence calculation, 1 * 36742, liver-type isozyme, sequence calculation | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
PGD2 11-ketoreductase | - |
Bos taurus |
PGFS | - |
Bos taurus |
PGH2 9,11-endoperoxidase | - |
Bos taurus |
prostaglandin F synthase | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Bos taurus |