Application | Comment | Organism |
---|---|---|
molecular biology | genome editing of human cell lines can be used to complement mouse KO models to validate the function of genes in differentiated tissues and cells | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
gene AKR1B1, cloning from genomic DNA, DNA and amino acid sequence determination and analysis, recombinant expression in immortalized human endometrial stromal cells, HIESC-2 cells, expression analysis in clones 16-1, 16-2, and 16-4. Clones 16-1 and 16-2 present with a complete absence of AKR1B1 protein, while clone 16-4 is able to express the AKR1B1 protein. Following treatment of cells with interleukin-1beta, both wild-type and clone 16-4 cells exhibit a similar response, but clone 16-2 does not respond with increased production of eitherPGF2alpha or PGE2. At the protein level, IL-1beta induces an increase in mPGES-1 and COX-2 protein expression but in clone 16-2 the relative increase of Cox-2 protein is weaker | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | knockout of AKR1B1 gene expression in human endometrial cell lines using the CRISPR/Cas9 system. Clone 16-2 exhibits deletion of 68 and 2 nucleotides, respectively, on each of the alleles. Cells from this clone lose their ability to produce PGF2alpha but maintain their original human endometrial stromal cells-2 phenotype including the capacity to decidualize in the presence of progesterone (medroxyprogesterone acetate) and 8-bromo-cAMP. Knockout cells also maintain their ability to increase PGE2 production in response to interleukin-1beta | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
prostaglandin H2 + NADP+ | Homo sapiens | - |
prostaglandin F2alpha + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P15121 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
uterine endometrium | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
prostaglandin H2 + NADP+ | - |
Homo sapiens | prostaglandin F2alpha + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AKR1B1 | - |
Homo sapiens |
aldo-ketoreductase 1B1 | - |
Homo sapiens |
More | cf. EC 1.1.1.21 | Homo sapiens |
PGF synthase | - |
Homo sapiens |
prostaglandin F2alpha synthase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | most prostaglandin F2alpha synthases (PGFS) identified to date are aldo-ketoreductases, AKRs | Homo sapiens |
metabolism | AKR1B1 is involved in the synthesis of PGF2alpha. Pathways of prostaglandin F2alpha biosynthesis in human cells, overview | Homo sapiens |
physiological function | prostaglandins (PGs) are important regulators of female reproductive function. The primary PGs produced in the endometrium are PGE2 and PGF2alpha. Role of aldo-ketoreductase (AKR)1B1 in increased PGF2alpha production by human endometrial cells following stimulation with interleukin-1beta (IL-1beta) | Homo sapiens |