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Literature summary for 1.1.1.188 extracted from

  • Lacroix Pepin, N.; Chapdelaine, P.; Rodriguez, Y.; Tremblay, J.P.; Fortier, M.A.
    Generation of human endometrial knockout cell lines with the CRISPR/Cas9 system confirms the prostaglandin F2alpha synthase activity of aldo-ketoreductase 1B1 (2014), Mol. Hum. Reprod., 20, 650-663.
    View publication on PubMed

Application

Application Comment Organism
molecular biology genome editing of human cell lines can be used to complement mouse KO models to validate the function of genes in differentiated tissues and cells Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
gene AKR1B1, cloning from genomic DNA, DNA and amino acid sequence determination and analysis, recombinant expression in immortalized human endometrial stromal cells, HIESC-2 cells, expression analysis in clones 16-1, 16-2, and 16-4. Clones 16-1 and 16-2 present with a complete absence of AKR1B1 protein, while clone 16-4 is able to express the AKR1B1 protein. Following treatment of cells with interleukin-1beta, both wild-type and clone 16-4 cells exhibit a similar response, but clone 16-2 does not respond with increased production of eitherPGF2alpha or PGE2. At the protein level, IL-1beta induces an increase in mPGES-1 and COX-2 protein expression but in clone 16-2 the relative increase of Cox-2 protein is weaker Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information knockout of AKR1B1 gene expression in human endometrial cell lines using the CRISPR/Cas9 system. Clone 16-2 exhibits deletion of 68 and 2 nucleotides, respectively, on each of the alleles. Cells from this clone lose their ability to produce PGF2alpha but maintain their original human endometrial stromal cells-2 phenotype including the capacity to decidualize in the presence of progesterone (medroxyprogesterone acetate) and 8-bromo-cAMP. Knockout cells also maintain their ability to increase PGE2 production in response to interleukin-1beta Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
prostaglandin H2 + NADP+ Homo sapiens
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prostaglandin F2alpha + NADPH + H+
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?

Organism

Organism UniProt Comment Textmining
Homo sapiens P15121
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-

Source Tissue

Source Tissue Comment Organism Textmining
uterine endometrium
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Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
prostaglandin H2 + NADP+
-
Homo sapiens prostaglandin F2alpha + NADPH + H+
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?

Synonyms

Synonyms Comment Organism
AKR1B1
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Homo sapiens
aldo-ketoreductase 1B1
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Homo sapiens
More cf. EC 1.1.1.21 Homo sapiens
PGF synthase
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Homo sapiens
prostaglandin F2alpha synthase
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Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Homo sapiens

General Information

General Information Comment Organism
evolution most prostaglandin F2alpha synthases (PGFS) identified to date are aldo-ketoreductases, AKRs Homo sapiens
metabolism AKR1B1 is involved in the synthesis of PGF2alpha. Pathways of prostaglandin F2alpha biosynthesis in human cells, overview Homo sapiens
physiological function prostaglandins (PGs) are important regulators of female reproductive function. The primary PGs produced in the endometrium are PGE2 and PGF2alpha. Role of aldo-ketoreductase (AKR)1B1 in increased PGF2alpha production by human endometrial cells following stimulation with interleukin-1beta (IL-1beta) Homo sapiens