Inhibitors | Comment | Organism | Structure |
---|---|---|---|
prostaglandin E2 | inhibits the reduction of 20alpha-hydroxy-pregn-4-en-3-one to progesterone competitively | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
9,10-phenanthrenequinone | - |
Oryctolagus cuniculus | |
0.0164 | - |
NADPH | - |
Oryctolagus cuniculus | |
0.028 | - |
20alpha-hydroxy-pregn-4-en-3-one | - |
Oryctolagus cuniculus | |
0.122 | - |
prostaglandin E2 | - |
Oryctolagus cuniculus | |
0.83 | - |
DL-glyceraldehyde | - |
Oryctolagus cuniculus | |
1.1 | - |
methylglyoxal | - |
Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Oryctolagus cuniculus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
gel filtration and SDS-PAGE | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
corpus luteum | pseudopregnant rabbits | Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.25 | - |
- |
Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+ | - |
Oryctolagus cuniculus | (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate + NADPH + H+ | - |
? | |
20alpha-hydroxy-pregn-4-en-3-one + NADP+ | - |
Oryctolagus cuniculus | progesterone + NADPH | - |
r | |
9,10-phenanthrenequinone + NADPH | - |
Oryctolagus cuniculus | ? | - |
? | |
DL-glyceraldehyde + NADPH + H+ | - |
Oryctolagus cuniculus | ? | - |
r | |
methylglyoxal + NADPH + H+ | - |
Oryctolagus cuniculus | ? | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | alpha, 1 * 36000, SDS-PAGE | Oryctolagus cuniculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Oryctolagus cuniculus |
8 | - |
assay of 20alpha-hydroxy-pregn-4-en-3-one | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Oryctolagus cuniculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
prostaglandin E2 | competitive inhibitor for the reduction of 20alpha-hydroxy-pregn-4-en-3-one to progesterone | Oryctolagus cuniculus |