General Stability | Organism |
---|---|
enzyme is quite unstable at the low protein concentrations less than 10 mg/ml, sulfhydryl reagents like dithiothreitol, 2-mercaptoethanol and reduced glutathione protect the enzyme from inactivation | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
NADP+ | - |
Sus scrofa | |
0.07 | - |
Prostaglandin D2 | - |
Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
2 * 28000, SDS-PAGE | Sus scrofa |
52000 | 62000 | gel filtration | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
prostaglandin D2 + NADP+ | Sus scrofa | - |
15-ketoprostaglandin D2 + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
swine | - |
Purification (Comment) | Organism |
---|---|
partially | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.001795 | - |
- |
Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
15-keto-DELTA13-prostaglandin + NADP+ | - |
Sus scrofa | ? + NADPH | - |
? | |
prostaglandin D2 + NADP+ | - |
Sus scrofa | 15-ketoprostaglandin D2 + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 28000, SDS-PAGE | Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | - |
enzyme assay | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
enzyme assay | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | utilizes this cofactor exclusively | Sus scrofa |