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Literature summary for 1.1.1.196 extracted from

  • Shimizu, T.; Watanabe, K.; Tokumoto, H.; Hayaishi, O.
    Isolation of NADP+-dependent PGD2-specific 15-hydroxyprostaglandin dehydrogenase from swine brain (1982), Methods Enzymol., 86, 147-152.
    View publication on PubMed

General Stability

General Stability Organism
enzyme is quite unstable at the low protein concentrations less than 10 mg/ml, sulfhydryl reagents like dithiothreitol, 2-mercaptoethanol and reduced glutathione protect the enzyme from inactivation Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
NADP+
-
Sus scrofa
0.07
-
Prostaglandin D2
-
Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
2 * 28000, SDS-PAGE Sus scrofa
52000 62000 gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
prostaglandin D2 + NADP+ Sus scrofa
-
15-ketoprostaglandin D2 + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
swine
-

Purification (Commentary)

Purification (Comment) Organism
partially Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.001795
-
-
Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
15-keto-DELTA13-prostaglandin + NADP+
-
Sus scrofa ? + NADPH
-
?
prostaglandin D2 + NADP+
-
Sus scrofa 15-ketoprostaglandin D2 + NADPH + H+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 28000, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
24
-
enzyme assay Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
enzyme assay Sus scrofa

Cofactor

Cofactor Comment Organism Structure
NADP+ utilizes this cofactor exclusively Sus scrofa