Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Salvia rosmarinus |
expression in Escherichia coli | Salvia officinalis |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.1 M HEPES/NaOH pH 7.0 to pH 7.8, 5/4 pentaerythritol propoxylate 25% (v/v), 30%, 35%, 0.1 M and 0.2 M KCl | Salvia rosmarinus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-borneol + NAD+ | Salvia rosmarinus | - |
(+)-camphor + NADH + H+ | - |
? | |
(+)-borneol + NAD+ | Pseudomonas sp. TCU-HL1 | - |
(+)-camphor + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. TCU-HL1 | - |
- |
- |
Salvia officinalis | - |
- |
- |
Salvia rosmarinus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-Trap column chromatography | Salvia rosmarinus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0029 | - |
with (-)-carveol as substrate, at pH 7.5 and 25°C | Salvia rosmarinus |
0.0032 | - |
with propan-1-ol as substrate, at pH 7.5 and 25°C | Pseudomonas sp. TCU-HL1 |
0.0033 | - |
with cyclohexanol as substrate, at pH 7.5 and 25°C | Salvia rosmarinus |
0.0033 | - |
with propan-1-ol as substrate, at pH 7.5 and 25°C | Salvia rosmarinus |
0.0039 | - |
with (+)-endo-norborneol as substrate, at pH 7.5 and 25°C | Salvia rosmarinus |
0.0044 | - |
with 3-methyl cyclohexanol as substrate, at pH 7.5 and 25°C | Salvia rosmarinus |
0.0046 | - |
with (+)-fenchol as substrate, at pH 7.5 and 25°C | Salvia rosmarinus |
0.0048 | - |
with (-)-exo-norborneol as substrate, at pH 7.5 and 25°C | Salvia rosmarinus |
0.005 | - |
with (-)-carveol as substrate, at pH 7.5 and 25°C | Pseudomonas sp. TCU-HL1 |
0.0062 | - |
with heptan-2-ol as substrate, at pH 7.5 and 25°C | Pseudomonas sp. TCU-HL1 |
0.0083 | - |
with 4-methyl cyclohexanol as substrate, at pH 7.5 and 25°C | Salvia rosmarinus |
0.0098 | - |
with heptan-2-ol as substrate, at pH 7.5 and 25°C | Salvia rosmarinus |
0.01 | - |
with 2-methyl cyclohexanol as substrate, at pH 7.5 and 25°C | Salvia rosmarinus |
0.011 | - |
with 4-methyl cyclohexanol as substrate, at pH 7.5 and 25°C | Pseudomonas sp. TCU-HL1 |
0.013 | - |
with octan-3-ol as substrate, at pH 7.5 and 25°C | Salvia rosmarinus |
0.018 | - |
with 3-methyl cyclohexanol as substrate, at pH 7.5 and 25°C | Pseudomonas sp. TCU-HL1 |
0.023 | - |
with 2-methyl cyclohexanol as substrate, at pH 7.5 and 25°C | Pseudomonas sp. TCU-HL1 |
0.024 | - |
with (+)-isoborneol as substrate, at pH 7.5 and 25°C | Salvia rosmarinus |
0.028 | - |
with (+)-endo-norborneol as substrate, at pH 7.5 and 25°C | Pseudomonas sp. TCU-HL1 |
0.028 | - |
with cyclohexanol as substrate, at pH 7.5 and 25°C | Pseudomonas sp. TCU-HL1 |
0.03 | - |
with (+)-fenchol as substrate, at pH 7.5 and 25°C | Pseudomonas sp. TCU-HL1 |
0.03 | - |
with (+/-)-borneol as substrate, at pH 7.5 and 25°C | Salvia rosmarinus |
0.043 | - |
with octan-3-ol as substrate, at pH 7.5 and 25°C | Pseudomonas sp. TCU-HL1 |
0.069 | - |
with (-)-exo-norborneol as substrate, at pH 7.5 and 25°C | Pseudomonas sp. TCU-HL1 |
0.115 | - |
with (+)-isoborneol as substrate, at pH 7.5 and 25°C | Pseudomonas sp. TCU-HL1 |
0.122 | - |
with (+/-)-borneol as substrate, at pH 7.5 and 25°C | Pseudomonas sp. TCU-HL1 |
9.8 | - |
isoform BDH1, substrate 2-hydroxyheptanol, pH 9, temperature not specified in the publication | Salvia officinalis |
10 | - |
isoform BDH1, substrate 2-methylcyclohexanol, pH 9, temperature not specified in the publication | Salvia officinalis |
10 | - |
isoform BDH2, substrate rac-borneol, pH 9, temperature not specified in the publication | Salvia officinalis |
13 | - |
isoform BDH1, substrate 6-hydroxyoctanol, pH 9, temperature not specified in the publication | Salvia officinalis |
16 | - |
isoform BDH2, substrate (-)-carveol, pH 9, temperature not specified in the publication | Salvia officinalis |
24 | - |
isoform BDH1, substrate isoborneol, pH 9, temperature not specified in the publication | Salvia officinalis |
30 | - |
isoform BDH1, substrate rac-borneol, pH 9, temperature not specified in the publication | Salvia officinalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-borneol + NAD+ | - |
Salvia officinalis | (+)-camphor + NADH + H+ | - |
? | |
(+)-borneol + NAD+ | - |
Salvia rosmarinus | (+)-camphor + NADH + H+ | - |
? | |
(+)-borneol + NAD+ | - |
Pseudomonas sp. TCU-HL1 | (+)-camphor + NADH + H+ | - |
? | |
(+)-endo-norborneol + NAD+ | - |
Salvia rosmarinus | (-)-exonorcamphor + NADH + H+ | - |
? | |
(+)-endo-norborneol + NAD+ | - |
Pseudomonas sp. TCU-HL1 | (-)-exonorcamphor + NADH + H+ | - |
? | |
(+)-fenchol + NAD+ | - |
Salvia rosmarinus | (-)-fenchone + NADH + H+ | - |
? | |
(+)-fenchol + NAD+ | - |
Pseudomonas sp. TCU-HL1 | (-)-fenchone + NADH + H+ | - |
? | |
(+)-isoborneol + NAD+ | - |
Salvia rosmarinus | (-)-camphor + NADH + H+ | - |
? | |
(+)-isoborneol + NAD+ | - |
Pseudomonas sp. TCU-HL1 | (-)-camphor + NADH + H+ | - |
? | |
(+/-)-borneol + NAD+ | - |
Salvia rosmarinus | (+/-)-camphor + NADH + H+ | - |
? | |
(+/-)-borneol + NAD+ | - |
Pseudomonas sp. TCU-HL1 | (+/-)-camphor + NADH + H+ | - |
? | |
(-)-carveol + NAD+ | - |
Salvia rosmarinus | ? | - |
? | |
(-)-carveol + NAD+ | - |
Pseudomonas sp. TCU-HL1 | ? | - |
? | |
(-)-cis-carveol + NAD+ | - |
Salvia officinalis | (-)-carvone + NADH + H+ | - |
? | |
(-)-exo-norborneol + NAD+ | - |
Salvia rosmarinus | (+)-exonorcamphor + NADH + H+ | - |
? | |
(-)-exo-norborneol + NAD+ | - |
Pseudomonas sp. TCU-HL1 | (+)-exonorcamphor + NADH + H+ | - |
? | |
2-hydroxyheptanol + NAD+ | - |
Salvia officinalis | 2-oxoheptanol + NADH + H+ | - |
? | |
2-methyl cyclohexanol + NAD+ | - |
Salvia rosmarinus | ? | - |
? | |
2-methyl cyclohexanol + NAD+ | - |
Pseudomonas sp. TCU-HL1 | ? | - |
? | |
2-methylcyclohexanol + NAD+ | - |
Salvia officinalis | 2-methylcyclohexanone + NADH + H+ | - |
? | |
3-hydroxyoctanol + NAD+ | - |
Salvia officinalis | 3-oxooctanol + NADH + H+ | - |
? | |
3-methyl cyclohexanol + NAD+ | - |
Salvia officinalis | 3-methycyclohexanone + NADH + H+ | - |
? | |
3-methyl cyclohexanol + NAD+ | - |
Salvia rosmarinus | ? | - |
? | |
3-methyl cyclohexanol + NAD+ | - |
Pseudomonas sp. TCU-HL1 | ? | - |
? | |
4-methyl cyclohexanol + NAD+ | - |
Salvia rosmarinus | ? | - |
? | |
4-methyl cyclohexanol + NAD+ | - |
Pseudomonas sp. TCU-HL1 | ? | - |
? | |
cyclohexanol + NAD+ | - |
Salvia rosmarinus | ? | - |
? | |
cyclohexanol + NAD+ | - |
Pseudomonas sp. TCU-HL1 | ? | - |
? | |
heptan-2-ol + NAD+ | - |
Salvia rosmarinus | ? | - |
? | |
heptan-2-ol + NAD+ | - |
Pseudomonas sp. TCU-HL1 | ? | - |
? | |
additional information | isoforms BDH1 and BDH2 also accept monocyclic and linear substrates, clearly favoring secondary alcohols over primary alcohols. No substrate: menthol | Salvia officinalis | ? | - |
- |
|
additional information | no activity with menthol | Salvia rosmarinus | ? | - |
- |
|
additional information | no activity with menthol | Pseudomonas sp. TCU-HL1 | ? | - |
- |
|
octan-3-ol + NAD+ | - |
Salvia rosmarinus | ? | - |
? | |
octan-3-ol + NAD+ | - |
Pseudomonas sp. TCU-HL1 | ? | - |
? | |
propan-1-ol + NAD+ | - |
Salvia rosmarinus | ? | - |
? | |
propan-1-ol + NAD+ | - |
Pseudomonas sp. TCU-HL1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BDH | - |
Pseudomonas sp. TCU-HL1 |
BDH1 | - |
Salvia officinalis |
BDH1 | - |
Salvia rosmarinus |
BDH2 | - |
Salvia officinalis |
borneol-type dehydrogenase 1 | - |
Salvia rosmarinus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Salvia rosmarinus |
7.5 | - |
- |
Pseudomonas sp. TCU-HL1 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10.5 | isoform BDH1, 30 min, more than 50% residual activity | Salvia officinalis |
5 | 10.5 | the enzyme retains over 50% activity for pH values between 5.0 and 10.5 after 30 min of incubation. After 24 h of incubation, the activity showed little variation for pH between 5.5 and 10.5 | Salvia rosmarinus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Salvia rosmarinus | |
NAD+ | - |
Pseudomonas sp. TCU-HL1 |