Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Transetta (DE3) cells | Cinnamomum camphora |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0251 | - |
(+)-borneol | isoform BDH3, at pH 8.5 and 30°C | Cinnamomum camphora | |
0.0369 | - |
(-)-borneol | isoform BDH3, at pH 9.0 and 32°C | Cinnamomum camphora |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-borneol + NAD+ | Cinnamomum camphora | - |
(+)-camphor + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cinnamomum camphora | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity column chromatography | Cinnamomum camphora |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Cinnamomum camphora | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-borneol + NAD+ | - |
Cinnamomum camphora | (+)-camphor + NADH + H+ | - |
? | |
(-)-borneol + NAD+ | - |
Cinnamomum camphora | (-)-camphor + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BDH3 | - |
Cinnamomum camphora |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
9 | - |
highest activity value for (-)-borneol at 32°C | Cinnamomum camphora |
30 | - |
highest activity value for (+)-borneol at pH 30°C | Cinnamomum camphora |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0021 | - |
(-)-borneol | isoform BDH3, at pH 9.0 and 32°C | Cinnamomum camphora | |
0.0054 | - |
(+)-borneol | isoform BDH3, at pH 8.5 and 30°C | Cinnamomum camphora |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
highest activity value for (+)-borneol at pH 8.5 | Cinnamomum camphora |
9 | - |
highest activity value for (-)-borneol at pH 9.0 | Cinnamomum camphora |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Cinnamomum camphora |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.056 | - |
(-)-borneol | isoform BDH3, at pH 9.0 and 32°C | Cinnamomum camphora | |
0.22 | - |
(+)-borneol | isoform BDH3, at pH 8.5 and 30°C | Cinnamomum camphora |