KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
NADPH | pH 8.0, 80°C | Pseudothermotoga hypogea | |
0.02 | - |
NADP+ | pH 11.0, 80°C | Pseudothermotoga hypogea | |
0.45 | - |
Butyraldehyde | pH 8.0, 80°C | Pseudothermotoga hypogea | |
1.9 | - |
butanol | pH 11.0, 80°C | Pseudothermotoga hypogea | |
3.1 | - |
acetaldehyde | pH 8.0, 80°C | Pseudothermotoga hypogea | |
9.7 | - |
ethanol | pH 11.0, 80°C | Pseudothermotoga hypogea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | a fully active enzyme contains iron of 1.02 gatoms/subunit | Pseudothermotoga hypogea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE | Pseudothermotoga hypogea |
70000 | - |
gel filtration | Pseudothermotoga hypogea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
aldehyde + NADPH + H+ | Pseudothermotoga hypogea | the physiological role of this enzyme is likely to catalyze the reduction of aldehydes to alcohols | alcohol + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudothermotoga hypogea | - |
- |
- |
Oxidation Stability | Organism |
---|---|
the enzyme is oxygen sensitive. Loss of enzyme activity by exposure to oxygen could be recovered by incubation with dithiothreitol and Fe2+ | Pseudothermotoga hypogea |
Purification (Comment) | Organism |
---|---|
- |
Pseudothermotoga hypogea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
57 | - |
- |
Pseudothermotoga hypogea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-propanediol + NADP+ | - |
Pseudothermotoga hypogea | ? | - |
? | |
1,4-butanediol + NADP+ | - |
Pseudothermotoga hypogea | ? | - |
? | |
1,5-pentanediol + NADP+ | - |
Pseudothermotoga hypogea | ? | - |
? | |
1-butanol + NADP+ | - |
Pseudothermotoga hypogea | butanal + NADPH + H+ | - |
? | |
1-butanol + NADP+ | - |
Pseudothermotoga hypogea | butyraldehyde + NADPH + H+ | - |
r | |
1-heptanol + NADP+ | - |
Pseudothermotoga hypogea | heptanal + NADPH + H+ | - |
? | |
1-octanol + NADP+ | - |
Pseudothermotoga hypogea | octanal + NADPH + H+ | - |
? | |
1-pentanol + NADP+ | - |
Pseudothermotoga hypogea | n-pentanal + NADPH + H+ | - |
? | |
1-propanol + NADP+ | - |
Pseudothermotoga hypogea | ? | - |
? | |
2-phenylethanol + NADP+ | - |
Pseudothermotoga hypogea | phenylacetaldehyde + NADPH + H+ | - |
? | |
aldehyde + NADPH + H+ | the physiological role of this enzyme is likely to catalyze the reduction of aldehydes to alcohols | Pseudothermotoga hypogea | alcohol + NADP+ | - |
? | |
ethanol + NADP+ | - |
Pseudothermotoga hypogea | acetaldehyde + NADPH + H+ | - |
r | |
hexyl alcohol + NADP+ | - |
Pseudothermotoga hypogea | hexanal + NADPH + H+ | - |
? | |
methylglyoxal + NADPH | - |
Pseudothermotoga hypogea | ? | - |
? | |
additional information | no activity with methanol, 2-propanol, 2-butanol and acetone | Pseudothermotoga hypogea | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, SDS-PAGE | Pseudothermotoga hypogea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
activity increases along with the rise of temperature up to 95°C | Pseudothermotoga hypogea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
half-life: 10 h | Pseudothermotoga hypogea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14 | - |
ethanol | pH 11.0, 80°C | Pseudothermotoga hypogea | |
15 | - |
acetaldehyde | pH 8.0, 80°C | Pseudothermotoga hypogea | |
48 | - |
butanol | pH 11.0, 80°C | Pseudothermotoga hypogea | |
48 | - |
Butyraldehyde | pH 8.0, 80°C | Pseudothermotoga hypogea | |
50 | - |
NADP+ | pH 11.0, 80°C | Pseudothermotoga hypogea | |
52 | - |
NADPH | pH 8.0, 80°C | Pseudothermotoga hypogea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
production of alcohol | Pseudothermotoga hypogea |
11 | - |
oxidation of alcohol | Pseudothermotoga hypogea |