Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, wild-type and mutant enzyme | Thermoanaerobacter brockii |
expression in Escherichia coli, wild-type and mutant enzyme | Entamoeba histolytica |
Crystallization (Comment) | Organism |
---|---|
crystals of D275PEhADH1 are grown using the hanging-drop vapor-diffusion method at 20°C. Crystal structure of the thermostabilized mutant D275P-EhADH1 suggests that a proline residue at position 275 thermostabilizes the enzymes by reducing flexibility and by reinforcing hydrophobic interactions at the dimerdimer interface of the tetrameric ADH | Entamoeba histolytica |
crystals of P275DTbADH1 are grown using the hanging-drop vapor-diffusion method at 20°C. Crystal structure of the thermostabilized mutant P275D-EhADH1 suggests that a proline residue at position 275 thermostabilizes the enzymes by reducing flexibility and by reinforcing hydrophobic interactions at the dimerdimer interface of the tetrameric ADH | Thermoanaerobacter brockii |
Protein Variants | Comment | Organism |
---|---|---|
D275P | mutation significantly enhances the thermal stability of EhADH1 | Entamoeba histolytica |
P275D | mutation reduces the thermostability of the enzyme | Thermoanaerobacter brockii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | P35630 | - |
- |
Thermoanaerobacter brockii | P14941 | - |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzyme | Thermoanaerobacter brockii |
wild-type and mutant enzyme | Entamoeba histolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-butanol + NADP+ | - |
Thermoanaerobacter brockii | ? | - |
? | |
2-butanol + NADP+ | - |
Entamoeba histolytica | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EhADH1 | - |
Entamoeba histolytica |
TbADH1 | - |
Thermoanaerobacter brockii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
76 | - |
1 h, 50% loss of activity of wild-type enzyme | Thermoanaerobacter brockii |
76 | - |
1 h, 50% loss of activity of wild-type enzyme | Entamoeba histolytica |
87 | - |
1 h, 50% loss of activity of mutant enzyme D275P | Thermoanaerobacter brockii |
87 | - |
1 h, 50% loss of activity of mutant enzyme D275P | Entamoeba histolytica |