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Literature summary for 1.1.1.2 extracted from

  • Alsafadi, D.; Paradisi, F.
    Effect of organic solvents on the activity and stability of halophilic alcohol dehydrogenase (ADH2) from Haloferax volcanii (2013), Extremophiles, 17, 115-122.
    View publication on PubMed
Search for more literature for 1.1.1.2

General Stability

General Stability Organism
high concentration of salt, essential to maintain the enzymatic activity and structural integrity of the halophilic enzyme under standard conditions may be partially replaced by DMSO and MeOH Haloferax volcanii

Metals/Ions

Metals/Ions Comment Organism Structure
KCl high concentration of salt, essential to maintain the enzymatic activity and structural integrity of the halophilic enzyme under standard conditions may be partially replaced by DMSO and MeOH Haloferax volcanii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ethanol + NADP+ Haloferax volcanii
-
 acetaldehyde + NADPH + H+
-
 r

Organic Solvent Stability

Organic Solvent Comment Organism
acetonitrile 62% remaining activity at 10% after 72 h at 5°C, 34% at 5°C, rapid inactivation at 50°C and 30% Haloferax volcanii
DMSO 78% remaining activity at 30% after 72 h at 5°C, inactivation within 24 h at 50°C and 30% Haloferax volcanii
Methanol at 5°C and 50°C, 38% remaining activity at 30%, 67% at 10%, and 70% at 5% after 72 h, inactivation within 24 h at 50°C and 30% Haloferax volcanii
additional information longterm effects of organic solvents at 5°C and 50°C, overview. High concentration of salt, essential to maintain the enzymatic activity and structural integrity of the halophilic enzyme under standard conditions may be partially replaced by DMSO and MeOH. Presence of organic solvents does not induce gross changes in substrate specificity Haloferax volcanii
tetrahydrofuran at 5°C, 32% activity remaining after 24 h, 7% after 72 h at 5% THF, 12% remaining activity at 10% THF after 24 h, inactivation within 72 h at 10% THF, and within 24 h at 30%THF Haloferax volcanii

Organism

Organism UniProt Comment Textmining
Haloferax volcanii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethanol + NADP+
-
 Haloferax volcanii acetaldehyde + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
ADH2
-
 Haloferax volcanii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Haloferax volcanii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
 oxidative reaction Haloferax volcanii

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Haloferax volcanii
NADPH
-
 Haloferax volcanii