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Literature summary for 1.1.1.2 extracted from

  • Liu, X.; Dong, Y.; Zhang, J.; Zhang, A.; Wang, L.; Feng, L.
    Two novel metal-independent long-chain alkyl alcohol dehydrogenases from Geobacillus thermodenitrificans NG80-2 (2009), Microbiology, 155, 2078-2085 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
EDTA 1 mM, 109.1% of initial activity Geobacillus thermodenitrificans

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Geobacillus thermodenitrificans

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 1 mM, 14.7% of initial activity Geobacillus thermodenitrificans
Ca2+ 1 mM, 0% of initial activity Geobacillus thermodenitrificans
Co2+ 1 mM, 68.7% of initial activity Geobacillus thermodenitrificans
Cu2+ 1 mM, 14.0% of initial activity Geobacillus thermodenitrificans
K+ 1 mM, 82.3% of initial activity Geobacillus thermodenitrificans
Mg2+ 1 mM, 0.3% of initial activity Geobacillus thermodenitrificans
Mn2+ 1 mM, 23.4% of initial activity Geobacillus thermodenitrificans
Ni2+ 1 mM, 18.2% of initial activity Geobacillus thermodenitrificans
SDS 1 mM,0% of initial activity Geobacillus thermodenitrificans
Zn2+ 1 mM, 14.1% of initial activity Geobacillus thermodenitrificans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.84
-
NADH cosubstrate octanal, pH 8, 60°C Geobacillus thermodenitrificans
1.15
-
octanal pH 8, 60°C Geobacillus thermodenitrificans
1.44
-
NAD+ cosubstrate octan-1-ol, pH 8, 60°C Geobacillus thermodenitrificans
2.25
-
acetaldehyde pH 8, 60°C Geobacillus thermodenitrificans
2.4
-
NADP+ cosubstrate octan-1-ol, pH 8, 60°C Geobacillus thermodenitrificans
3.88
-
octan-1-ol pH 8, 60°C Geobacillus thermodenitrificans
37.6
-
ethanol pH 8, 60°C Geobacillus thermodenitrificans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 1 mM, 168.9% of initial activity Geobacillus thermodenitrificans
additional information purified enzyme does not contain a significant amount of Fe, Ca, Co, Cu, Mg, Mn or Zn Geobacillus thermodenitrificans
Na+ 1 mM, 103.8% of initial activity Geobacillus thermodenitrificans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
PAGE Geobacillus thermodenitrificans

Organism

Organism UniProt Comment Textmining
Geobacillus thermodenitrificans A4ISB9 cf. EC 1.1.1.192
-
Geobacillus thermodenitrificans NG80-2 A4ISB9 cf. EC 1.1.1.192
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, purification by nickel ion affinity chromatography Geobacillus thermodenitrificans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
363.6
-
substrate octan-1-ol, cosubstrate NADP+, pH 8, 60°C Geobacillus thermodenitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-propanediol + 2 NADP+ + H2O
-
Geobacillus thermodenitrificans propanedial + 2 NADPH + 2 H+
-
r
acetaldehyde + NADPH + H+
-
Geobacillus thermodenitrificans ethanol + NADP+
-
r
acetaldehyde + NADPH + H+
-
Geobacillus thermodenitrificans NG80-2 ethanol + NADP+
-
r
acetone + NADP+
-
Geobacillus thermodenitrificans propan-2-ol + NADPH + H+
-
r
butan-1-ol + NADP+
-
Geobacillus thermodenitrificans butanal + NADPH + H+
-
r
butan-1-ol + NADP+
-
Geobacillus thermodenitrificans NG80-2 butanal + NADPH + H+
-
r
decan-1-ol + NADP+
-
Geobacillus thermodenitrificans decanal + NADPH + H+
-
r
decanal + NADP+
-
Geobacillus thermodenitrificans decan-1-ol + NADPH + H+
-
r
dodecan-1-ol + NADP+
-
Geobacillus thermodenitrificans dodecanal + NADPH + H+
-
r
dodecanal + NADP+
-
Geobacillus thermodenitrificans dodecan-1-ol + NADPH + H+
-
r
eicosan-1-ol + NADP+
-
Geobacillus thermodenitrificans n-eicosanal + NADPH + H+
-
r
ethanol + NADP+ ethanol is the best substrate Geobacillus thermodenitrificans acetaldehyde + NADPH + H+
-
r
ethanol + NADP+ ethanol is the best substrate Geobacillus thermodenitrificans NG80-2 acetaldehyde + NADPH + H+
-
r
formaldehyde + NADPH + H+
-
Geobacillus thermodenitrificans methanol + NADP+
-
r
glycerol + NADP+
-
Geobacillus thermodenitrificans dihydroxyacetone + NADPH + H+
-
r
glycerol + NADP+
-
Geobacillus thermodenitrificans NG80-2 dihydroxyacetone + NADPH + H+
-
r
hexadecan-1-ol + NADP+
-
Geobacillus thermodenitrificans hexadecanal + NADPH + H+
-
r
hexan-1-ol + NADP+
-
Geobacillus thermodenitrificans hexanal + NADPH + H+
-
r
isoamylalcohol + NADP+
-
Geobacillus thermodenitrificans 3-methylbutanal + NADPH + H+
-
r
isopropanol + NADP+
-
Geobacillus thermodenitrificans propan-2-one + NADPH + H+
-
r
methanol + NAD+
-
Geobacillus thermodenitrificans formaldehyde + NADH + H+
-
r
methanol + NAD+
-
Geobacillus thermodenitrificans NG80-2 formaldehyde + NADH + H+
-
r
octacosan-1-ol + NADP+
-
Geobacillus thermodenitrificans octacosanal + NADPH + H+
-
r
octadecan-1-ol + NADP+
-
Geobacillus thermodenitrificans octadecanal + NADPH + H+
-
r
octan-1-ol + NADP+
-
Geobacillus thermodenitrificans octanal + NADPH + H+
-
r
octanal + NADP+
-
Geobacillus thermodenitrificans octan-1-ol + NADPH + H+
-
r
tetracosanol-1-ol + NADP+
-
Geobacillus thermodenitrificans tetracosanal + NADPH + H+
-
r
tetradecan-1-ol + NADP+
-
Geobacillus thermodenitrificans tetradecanal + NADPH + H+
-
r
triacontan-1-ol + NADP+
-
Geobacillus thermodenitrificans triacontanal + NADPH + H+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 45000, SDS-PAGE, 2 * 42804, calculated from sequence Geobacillus thermodenitrificans

Synonyms

Synonyms Comment Organism
ADH2
-
Geobacillus thermodenitrificans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Geobacillus thermodenitrificans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 65
-
Geobacillus thermodenitrificans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
14 h, more than 50% of initial activity Geobacillus thermodenitrificans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
38.5
-
NAD+ cosubstrate octan-1-ol, pH 8, 60°C Geobacillus thermodenitrificans
136.2
-
ethanol pH 8, 60°C Geobacillus thermodenitrificans
138.1
-
acetaldehyde pH 8, 60°C Geobacillus thermodenitrificans
260.7
-
octan-1-ol pH 8, 60°C Geobacillus thermodenitrificans
283.1
-
NADP+ cosubstrate octan-1-ol, pH 8, 60°C Geobacillus thermodenitrificans
518.4
-
octanal pH 8, 60°C Geobacillus thermodenitrificans
4808.2
-
NADH cosubstrate octanal, pH 8, 60°C Geobacillus thermodenitrificans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Geobacillus thermodenitrificans

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
Geobacillus thermodenitrificans

Cofactor

Cofactor Comment Organism Structure
NAD+ NADP+ is about 4fold preferred over NADP+ Geobacillus thermodenitrificans
NADP+ NADP+ is about 4fold preferred over NADP+ Geobacillus thermodenitrificans
NADPH
-
Geobacillus thermodenitrificans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.62
-
ethanol pH 8, 60°C Geobacillus thermodenitrificans
26.7
-
NAD+ cosubstrate octan-1-ol, pH 8, 60°C Geobacillus thermodenitrificans
61.4
-
acetaldehyde pH 8, 60°C Geobacillus thermodenitrificans
67.2
-
octan-1-ol pH 8, 60°C Geobacillus thermodenitrificans
119.9
-
NADP+ cosubstrate octan-1-ol, pH 8, 60°C Geobacillus thermodenitrificans
450.8
-
octanal pH 8, 60°C Geobacillus thermodenitrificans
5724
-
NADH cosubstrate octanal, pH 8, 60°C Geobacillus thermodenitrificans