Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, 109.1% of initial activity | Geobacillus thermodenitrificans |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Geobacillus thermodenitrificans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 1 mM, 14.7% of initial activity | Geobacillus thermodenitrificans | |
Ca2+ | 1 mM, 0% of initial activity | Geobacillus thermodenitrificans | |
Co2+ | 1 mM, 68.7% of initial activity | Geobacillus thermodenitrificans | |
Cu2+ | 1 mM, 14.0% of initial activity | Geobacillus thermodenitrificans | |
K+ | 1 mM, 82.3% of initial activity | Geobacillus thermodenitrificans | |
Mg2+ | 1 mM, 0.3% of initial activity | Geobacillus thermodenitrificans | |
Mn2+ | 1 mM, 23.4% of initial activity | Geobacillus thermodenitrificans | |
Ni2+ | 1 mM, 18.2% of initial activity | Geobacillus thermodenitrificans | |
SDS | 1 mM,0% of initial activity | Geobacillus thermodenitrificans | |
Zn2+ | 1 mM, 14.1% of initial activity | Geobacillus thermodenitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.84 | - |
NADH | cosubstrate octanal, pH 8, 60°C | Geobacillus thermodenitrificans | |
1.15 | - |
octanal | pH 8, 60°C | Geobacillus thermodenitrificans | |
1.44 | - |
NAD+ | cosubstrate octan-1-ol, pH 8, 60°C | Geobacillus thermodenitrificans | |
2.25 | - |
acetaldehyde | pH 8, 60°C | Geobacillus thermodenitrificans | |
2.4 | - |
NADP+ | cosubstrate octan-1-ol, pH 8, 60°C | Geobacillus thermodenitrificans | |
3.88 | - |
octan-1-ol | pH 8, 60°C | Geobacillus thermodenitrificans | |
37.6 | - |
ethanol | pH 8, 60°C | Geobacillus thermodenitrificans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 1 mM, 168.9% of initial activity | Geobacillus thermodenitrificans | |
additional information | purified enzyme does not contain a significant amount of Fe, Ca, Co, Cu, Mg, Mn or Zn | Geobacillus thermodenitrificans | |
Na+ | 1 mM, 103.8% of initial activity | Geobacillus thermodenitrificans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
PAGE | Geobacillus thermodenitrificans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermodenitrificans | A4ISB9 | cf. EC 1.1.1.192 | - |
Geobacillus thermodenitrificans NG80-2 | A4ISB9 | cf. EC 1.1.1.192 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, purification by nickel ion affinity chromatography | Geobacillus thermodenitrificans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
363.6 | - |
substrate octan-1-ol, cosubstrate NADP+, pH 8, 60°C | Geobacillus thermodenitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-propanediol + 2 NADP+ + H2O | - |
Geobacillus thermodenitrificans | propanedial + 2 NADPH + 2 H+ | - |
r | |
acetaldehyde + NADPH + H+ | - |
Geobacillus thermodenitrificans | ethanol + NADP+ | - |
r | |
acetaldehyde + NADPH + H+ | - |
Geobacillus thermodenitrificans NG80-2 | ethanol + NADP+ | - |
r | |
acetone + NADP+ | - |
Geobacillus thermodenitrificans | propan-2-ol + NADPH + H+ | - |
r | |
butan-1-ol + NADP+ | - |
Geobacillus thermodenitrificans | butanal + NADPH + H+ | - |
r | |
butan-1-ol + NADP+ | - |
Geobacillus thermodenitrificans NG80-2 | butanal + NADPH + H+ | - |
r | |
decan-1-ol + NADP+ | - |
Geobacillus thermodenitrificans | decanal + NADPH + H+ | - |
r | |
decanal + NADP+ | - |
Geobacillus thermodenitrificans | decan-1-ol + NADPH + H+ | - |
r | |
dodecan-1-ol + NADP+ | - |
Geobacillus thermodenitrificans | dodecanal + NADPH + H+ | - |
r | |
dodecanal + NADP+ | - |
Geobacillus thermodenitrificans | dodecan-1-ol + NADPH + H+ | - |
r | |
eicosan-1-ol + NADP+ | - |
Geobacillus thermodenitrificans | n-eicosanal + NADPH + H+ | - |
r | |
ethanol + NADP+ | ethanol is the best substrate | Geobacillus thermodenitrificans | acetaldehyde + NADPH + H+ | - |
r | |
ethanol + NADP+ | ethanol is the best substrate | Geobacillus thermodenitrificans NG80-2 | acetaldehyde + NADPH + H+ | - |
r | |
formaldehyde + NADPH + H+ | - |
Geobacillus thermodenitrificans | methanol + NADP+ | - |
r | |
glycerol + NADP+ | - |
Geobacillus thermodenitrificans | dihydroxyacetone + NADPH + H+ | - |
r | |
glycerol + NADP+ | - |
Geobacillus thermodenitrificans NG80-2 | dihydroxyacetone + NADPH + H+ | - |
r | |
hexadecan-1-ol + NADP+ | - |
Geobacillus thermodenitrificans | hexadecanal + NADPH + H+ | - |
r | |
hexan-1-ol + NADP+ | - |
Geobacillus thermodenitrificans | hexanal + NADPH + H+ | - |
r | |
isoamylalcohol + NADP+ | - |
Geobacillus thermodenitrificans | 3-methylbutanal + NADPH + H+ | - |
r | |
isopropanol + NADP+ | - |
Geobacillus thermodenitrificans | propan-2-one + NADPH + H+ | - |
r | |
methanol + NAD+ | - |
Geobacillus thermodenitrificans | formaldehyde + NADH + H+ | - |
r | |
methanol + NAD+ | - |
Geobacillus thermodenitrificans NG80-2 | formaldehyde + NADH + H+ | - |
r | |
octacosan-1-ol + NADP+ | - |
Geobacillus thermodenitrificans | octacosanal + NADPH + H+ | - |
r | |
octadecan-1-ol + NADP+ | - |
Geobacillus thermodenitrificans | octadecanal + NADPH + H+ | - |
r | |
octan-1-ol + NADP+ | - |
Geobacillus thermodenitrificans | octanal + NADPH + H+ | - |
r | |
octanal + NADP+ | - |
Geobacillus thermodenitrificans | octan-1-ol + NADPH + H+ | - |
r | |
tetracosanol-1-ol + NADP+ | - |
Geobacillus thermodenitrificans | tetracosanal + NADPH + H+ | - |
r | |
tetradecan-1-ol + NADP+ | - |
Geobacillus thermodenitrificans | tetradecanal + NADPH + H+ | - |
r | |
triacontan-1-ol + NADP+ | - |
Geobacillus thermodenitrificans | triacontanal + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 45000, SDS-PAGE, 2 * 42804, calculated from sequence | Geobacillus thermodenitrificans |
Synonyms | Comment | Organism |
---|---|---|
ADH2 | - |
Geobacillus thermodenitrificans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Geobacillus thermodenitrificans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 65 | - |
Geobacillus thermodenitrificans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
14 h, more than 50% of initial activity | Geobacillus thermodenitrificans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
38.5 | - |
NAD+ | cosubstrate octan-1-ol, pH 8, 60°C | Geobacillus thermodenitrificans | |
136.2 | - |
ethanol | pH 8, 60°C | Geobacillus thermodenitrificans | |
138.1 | - |
acetaldehyde | pH 8, 60°C | Geobacillus thermodenitrificans | |
260.7 | - |
octan-1-ol | pH 8, 60°C | Geobacillus thermodenitrificans | |
283.1 | - |
NADP+ | cosubstrate octan-1-ol, pH 8, 60°C | Geobacillus thermodenitrificans | |
518.4 | - |
octanal | pH 8, 60°C | Geobacillus thermodenitrificans | |
4808.2 | - |
NADH | cosubstrate octanal, pH 8, 60°C | Geobacillus thermodenitrificans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Geobacillus thermodenitrificans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Geobacillus thermodenitrificans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | NADP+ is about 4fold preferred over NADP+ | Geobacillus thermodenitrificans | |
NADP+ | NADP+ is about 4fold preferred over NADP+ | Geobacillus thermodenitrificans | |
NADPH | - |
Geobacillus thermodenitrificans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.62 | - |
ethanol | pH 8, 60°C | Geobacillus thermodenitrificans | |
26.7 | - |
NAD+ | cosubstrate octan-1-ol, pH 8, 60°C | Geobacillus thermodenitrificans | |
61.4 | - |
acetaldehyde | pH 8, 60°C | Geobacillus thermodenitrificans | |
67.2 | - |
octan-1-ol | pH 8, 60°C | Geobacillus thermodenitrificans | |
119.9 | - |
NADP+ | cosubstrate octan-1-ol, pH 8, 60°C | Geobacillus thermodenitrificans | |
450.8 | - |
octanal | pH 8, 60°C | Geobacillus thermodenitrificans | |
5724 | - |
NADH | cosubstrate octanal, pH 8, 60°C | Geobacillus thermodenitrificans |