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Literature summary for 1.1.1.200 extracted from
- Inhibition of recombinant aldose-6-phosphate reductase from peach leaves by hexose-phosphates, inorganic phosphate and oxidants (2017), Plant Cell Physiol., 58, 145-155 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 Star (DE3) cells | Prunus persica |
General Stability
| General Stability | Organism |
|---|---|
| enzyme thiolation with oxidized glutathione protects the enzyme from insolubilization caused by diamide, while incubation with NADP+ completely prevents enzyme precipitation | Prunus persica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 1,6-bisphosphate | about 40% inhibition at 20 mM | Prunus persica |  |
| D-fructose 6-phosphate | about 40% inhibition at 20 mM | Prunus persica | |
| D-galactose 1-phosphate | about 40% inhibition at 20 mM | Prunus persica | |
| D-glucose 1-phosphate | about 55% inhibition at 20 mM | Prunus persica | |
| D-ribose 5-phosphate | about 40% inhibition at 20 mM | Prunus persica | |
| Diamide | enzymatic activity decreases to approximately 7% at 500 mM diamide | Prunus persica | |
| H2O2 | H2O2 oxidation causes enzyme inactivation in a time- and concentration-dependent manner | Prunus persica | |
| mannose 1-phosphate | about 40% inhibition at 20 mM | Prunus persica | |
| mannose 6-phosphate | about 30% inhibition at 20 mM | Prunus persica | |
| additional information | no inhibition by D-trehalose 6-phosphate, D-fructose 2,6-bisphosphate, D-xylitol, D-glucose and D-fructose. Not affected by 5 mM GSSG | Prunus persica | |
| phosphate | competitive inhibitor | Prunus persica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.031 | - |
NADPH | at pH 8.0 and 25°C | Prunus persica | |
| 0.059 | - |
NADP+ | at pH 8.0 and 25°C | Prunus persica | |
| 3.5 | - |
D-glucose 6-phosphate | at pH 8.0 and 25°C | Prunus persica | |
| 11 | - |
D-glucose 6-phosphate | at pH 8.0 and 25°C | Prunus persica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no significant effects are observed when the activity of the enzyme is assayed in the presence of 5 mM Mg2+ or Ca2+ | Prunus persica |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
gel filtration | Prunus persica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + NADPH + H+ | Prunus persica | - |
D-sorbitol 6-phosphate + NADP+ | - |
r | |
| D-sorbitol 6-phosphate + NADP+ | Prunus persica | - |
D-glucose 6-phosphate + NADPH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Prunus persica | A5JUQ9 | cultivar Flordakin | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| NADP+ protects the enzyme from oxidative insolubilization | Prunus persica |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisTrap column chromatography | Prunus persica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Prunus persica | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + NADPH + H+ | - |
Prunus persica | D-sorbitol 6-phosphate + NADP+ | - |
r | |
| D-sorbitol 6-phosphate + NADP+ | - |
Prunus persica | D-glucose 6-phosphate + NADPH + H+ | - |
r | |
| additional information | almost no activity with mannose 6-phosphate or ribose 5-phosphate, no activity with NADH and NAD+ | Prunus persica | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 35000, SDS-PAGE | Prunus persica |
| homodimer | 2 * 34800, calculated from amino acid sequence | Prunus persica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Ald6PRase | - |
Prunus persica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Prunus persica | |
| NADPH | - |
Prunus persica | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.07 | - |
D-glucose 1-phosphate | at pH 8.0 and 25°C | Prunus persica | |
| 26 | - |
D-fructose 1,6-bisphosphate | at pH 8.0 and 25°C | Prunus persica | |
| 29.6 | - |
phosphate | at pH 8.0 and 25°C | Prunus persica | |
| 55.4 | - |
D-fructose 6-phosphate | at pH 8.0 and 25°C | Prunus persica | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Prunus persica | calculated from amino acid sequence | - |
7.9 |
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