Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.202 extracted from

  • Tang, X.; Tan, Y.; Zhu, H.; Zhao, K.; Shen, W.
    Microbial conversion of glycerol to 1,3-propanediol by an engineered strain of Escherichia coli (2009), Appl. Environ. Microbiol., 75, 1628-1634.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
glycerol
-
Escherichia coli

Application

Application Comment Organism
industry development of an economical and eco-friendly biological process for the production of propane-1,3-diol from renewable resources by construction of a novel operon including YqhD Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
genes for the production of propane-1,3-diol in Clostridium butyricum, dhaB1 and dhaB2, which encode the vitamin B12-independent glycerol dehydratase DhaB1 and its activating factor, DhaB2, respectively, tandemly arrayed with the Escherichia coli yqhD gene, which encodes the 1,3-propanediol oxidoreductase isoenzyme YqhD. Heterologous expression of the propane-1,3-diol operon under the control of the temperature-sensitive lambda phage PLPR promoter regulated by the cIts857 repressor, from plasmid pDY220, in Escherichia coli K-12 ER2925 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli SYU 21132
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxypropanal + NADPH + H+ improvement of propane-1,3-diol production by a novel propane-1,3-diol operon of three genes (dhaB1 and dhaB2 from Clostridium butyricum and YqhD from Escherichia coli) tandemly arrayed under the control of a constitutive, temperature-sensitive promoter in the vector pBV220 for heterologous expression in Escherichia coli Escherichia coli propane-1,3-diol + NADP+
-
?
3-hydroxypropanal + NADPH + H+ improvement of propane-1,3-diol production by a novel propane-1,3-diol operon of three genes (dhaB1 and dhaB2 from Clostridium butyricum and YqhD from Escherichia coli) tandemly arrayed under the control of a constitutive, temperature-sensitive promoter in the vector pBV220 for heterologous expression in Escherichia coli Escherichia coli SYU 21132 propane-1,3-diol + NADP+
-
?

Synonyms

Synonyms Comment Organism
1,3-propanediol oxidoreductase
-
Escherichia coli
YqhD
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Escherichia coli