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Literature summary for 1.1.1.203 extracted from

  • Bateman, D.F.; Kosuge, T.; Kilgore, W.W.
    Purification and properties of uronate dehydrogenase from Pseudomonas syringae (1970), Arch. Biochem. Biophys., 136, 97-105.
    View publication on PubMed

General Stability

General Stability Organism
NAD+ increases stability Pseudomonas syringae

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ complete inhibition at 1 mM Pseudomonas syringae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.057
-
D-galacturonate
-
Pseudomonas syringae
0.15
-
D-glucuronate
-
Pseudomonas syringae
5.3
-
NAD+ with 0.67 mM D-galacturonate Pseudomonas syringae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-galacturonate + NAD+ + H2O Pseudomonas syringae 50% of the activity compared to D-glucoronate D-galactarate + NADH + H+
-
ir
D-glucuronate + NAD+ + H2O Pseudomonas syringae strong substrate specificity, best substrate D-glucaric acid + NADH + H+
-
ir

Organism

Organism UniProt Comment Textmining
Pseudomonas syringae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Pseudomonas syringae

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Pseudomonas syringae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
5.2
-
-
Pseudomonas syringae

Storage Stability

Storage Stability Organism
-10┬░C, 50 mM Tris-HCl, pH 7.2, 2.9 mM NAD+, less then 10% loss of activity up to 6 months Pseudomonas syringae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galacturonate + NAD+ + H2O 50% of the activity compared to D-glucoronate Pseudomonas syringae D-galactarate + NADH + H+
-
ir
D-glucuronate + NAD+ + H2O strong substrate specificity, best substrate Pseudomonas syringae D-glucaric acid + NADH + H+
-
ir

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
25
-
stable Pseudomonas syringae
30 35 loss of activity after 30 min Pseudomonas syringae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.4
-
Pseudomonas syringae

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5
-
50% remaining activity Pseudomonas syringae
9.4
-
50% remaining activity Pseudomonas syringae

Cofactor

Cofactor Comment Organism Structure
NAD+ can not be replaced by NADP+ Pseudomonas syringae