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Literature summary for 1.1.1.203 extracted from

  • Wagschal, K.; Chan, V.; Pereira, J.; Zwart, P.; Sankaran, B.
    Chromohalobacter salixigens uronate dehydrogenase Directed evolution for improved thermal stability and mutant CsUDH-inc X-ray crystal structure (2022), Process Biochem., 114, 185-192.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.203

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Chromohalobacter salexigens

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.1 M MES pH6.5 and 30% (w/v) 2-methyl-2-4-pentanediol Chromohalobacter salexigens

Protein Variants

Protein Variants Comment Organism
A172V the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme Chromohalobacter salexigens
D246N the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme Chromohalobacter salexigens
I114T the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme Chromohalobacter salexigens
I88L the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme Chromohalobacter salexigens
L158A/N159C/I160V the mutant shows increased catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme Chromohalobacter salexigens
M175I the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme Chromohalobacter salexigens
N99K/L100H/K102V/P103K/I105V the mutant shows wild type activity with beta-D-glucuronate and reduced catalytic efficiency with beta-D-galacturonate compared to the wild type enzyme Chromohalobacter salexigens
N99K/L100H/K102V/P103K/I105V/Y116F/T113V/A172V/M175I/D236N the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme Chromohalobacter salexigens
Q55R the mutant shows wild type activity with beta-D-glucuronate and reduced catalytic efficiency with beta-D-galacturonate compared to the wild type enzyme Chromohalobacter salexigens
Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175I/D246N the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme Chromohalobacter salexigens
T113V the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme Chromohalobacter salexigens
T113V/A172V/D246N the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme Chromohalobacter salexigens
T113V/A172V/M175I the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme Chromohalobacter salexigens
T113V/A172V/M175I/D236N the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme Chromohalobacter salexigens
T113V/M175I/D246N the mutant shows increased catalytic efficiency with beta-D-glucuronate and decreased catalytic efficiency with beta-D-galacturonate compared to the wild type enzyme Chromohalobacter salexigens
Y116F the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme Chromohalobacter salexigens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 beta-D-galacturonate mutant enzyme N99K/L100H/K102V/P103K/I105V, at pH 8.0 and 25°C Chromohalobacter salexigens
0.14
-
 beta-D-galacturonate mutant enzyme L158A/N159C/I160V, at pH 8.0 and 25°C Chromohalobacter salexigens
0.15
-
 beta-D-galacturonate mutant enzyme T113V, at pH 8.0 and 25°C Chromohalobacter salexigens
0.16
-
 beta-D-galacturonate mutant enzyme N99K/L100H/K102V/P103K/I105V/Y116F/T113V/A172V/M175I/D236N, at pH 8.0 and 25°C Chromohalobacter salexigens
0.18
-
 beta-D-galacturonate wild type enzyme, at pH 8.0 and 25°C Chromohalobacter salexigens
0.18
-
 beta-D-galacturonate mutant enzyme A172V, at pH 8.0 and 25°C Chromohalobacter salexigens
0.18
-
 beta-D-galacturonate mutant enzyme D246N, at pH 8.0 and 25°C Chromohalobacter salexigens
0.18
-
 beta-D-galacturonate mutant enzyme M175I, at pH 8.0 and 25°C Chromohalobacter salexigens
0.18
-
 beta-D-galacturonate mutant enzyme T113V/A172V/M175I, at pH 8.0 and 25°C Chromohalobacter salexigens
0.18
-
 beta-D-galacturonate mutant enzyme T113V/A172V/M175I/D236N, at pH 8.0 and 25°C Chromohalobacter salexigens
0.19
-
 beta-D-galacturonate mutant enzyme Q55R, at pH 8.0 and 25°C Chromohalobacter salexigens
0.19
-
 beta-D-galacturonate mutant enzyme T113V/M175I/D246N, at pH 8.0 and 25°C Chromohalobacter salexigens
0.2
-
 beta-D-galacturonate mutant enzyme I88L, at pH 8.0 and 25°C Chromohalobacter salexigens
0.21
-
 beta-D-galacturonate mutant enzyme Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175I/D246N, at pH 8.0 and 25°C Chromohalobacter salexigens
0.22
-
 beta-D-galacturonate mutant enzyme T113V/A172V/D246N, at pH 8.0 and 25°C Chromohalobacter salexigens
0.22
-
 beta-D-galacturonate mutant enzyme Y116F, at pH 8.0 and 25°C Chromohalobacter salexigens
0.41
-
 beta-D-galacturonate mutant enzyme I114T, at pH 8.0 and 25°C Chromohalobacter salexigens
0.43
-
 beta-D-glucuronate mutant enzyme N99K/L100H/K102V/P103K/I105V/Y116F/T113V/A172V/M175I/D236N, at pH 8.0 and 25°C Chromohalobacter salexigens
0.65
-
 beta-D-glucuronate mutant enzyme Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175I/D246N, at pH 8.0 and 25°C Chromohalobacter salexigens
0.9
-
 beta-D-glucuronate mutant enzyme N99K/L100H/K102V/P103K/I105V, at pH 8.0 and 25°C Chromohalobacter salexigens
0.95
-
 beta-D-glucuronate mutant enzyme T113V/A172V/M175I/D236N, at pH 8.0 and 25°C Chromohalobacter salexigens
1.03
-
 beta-D-glucuronate mutant enzyme M175I, at pH 8.0 and 25°C Chromohalobacter salexigens
1.1
-
 beta-D-glucuronate mutant enzyme T113V/A172V/M175I, at pH 8.0 and 25°C Chromohalobacter salexigens
1.1
-
 beta-D-glucuronate mutant enzyme T113V/M175I/D246N, at pH 8.0 and 25°C Chromohalobacter salexigens
1.2
-
 beta-D-glucuronate mutant enzyme L158A/N159C/I160V, at pH 8.0 and 25°C Chromohalobacter salexigens
1.3
-
 beta-D-glucuronate mutant enzyme D246N, at pH 8.0 and 25°C Chromohalobacter salexigens
1.5
-
 beta-D-glucuronate mutant enzyme I88L, at pH 8.0 and 25°C Chromohalobacter salexigens
1.5
-
 beta-D-glucuronate mutant enzyme T113V/A172V/D246N, at pH 8.0 and 25°C Chromohalobacter salexigens
1.6
-
 beta-D-glucuronate wild type enzyme, at pH 8.0 and 25°C Chromohalobacter salexigens
1.7
-
 beta-D-glucuronate mutant enzyme A172V, at pH 8.0 and 25°C Chromohalobacter salexigens
1.7
-
 beta-D-glucuronate mutant enzyme Q55R, at pH 8.0 and 25°C Chromohalobacter salexigens
1.7
-
 beta-D-glucuronate mutant enzyme T113V, at pH 8.0 and 25°C Chromohalobacter salexigens
2
-
 beta-D-glucuronate mutant enzyme Y116F, at pH 8.0 and 25°C Chromohalobacter salexigens
3.2
-
 beta-D-glucuronate mutant enzyme I114T, at pH 8.0 and 25°C Chromohalobacter salexigens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-D-galacturonate + NAD+ Chromohalobacter salexigens
-
 D-galactaro-1,5-lactone + NADH + H+
-
 ?
beta-D-glucuronate + NAD+ Chromohalobacter salexigens
-
 D-glucaro-1,5-lactone + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Chromohalobacter salexigens Q1QUN7
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Chromohalobacter salexigens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-galacturonate + NAD+
-
 Chromohalobacter salexigens D-galactaro-1,5-lactone + NADH + H+
-
 ?
beta-D-glucuronate + NAD+
-
 Chromohalobacter salexigens D-glucaro-1,5-lactone + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
Udh
-
 Chromohalobacter salexigens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 8 beta-D-galacturonate mutant enzyme Q55R, at pH 8.0 and 25°C Chromohalobacter salexigens
17
-
 beta-D-galacturonate mutant enzyme I114T, at pH 8.0 and 25°C Chromohalobacter salexigens
17
-
 beta-D-galacturonate mutant enzyme N99K/L100H/K102V/P103K/I105V, at pH 8.0 and 25°C Chromohalobacter salexigens
17
-
 beta-D-galacturonate mutant enzyme Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175I/D246N, at pH 8.0 and 25°C Chromohalobacter salexigens
19
-
 beta-D-galacturonate mutant enzyme T113V/A172V/M175I, at pH 8.0 and 25°C Chromohalobacter salexigens
20
-
 beta-D-galacturonate mutant enzyme M175I, at pH 8.0 and 25°C Chromohalobacter salexigens
20
-
 beta-D-galacturonate mutant enzyme N99K/L100H/K102V/P103K/I105V/Y116F/T113V/A172V/M175I/D236N, at pH 8.0 and 25°C Chromohalobacter salexigens
20
-
 beta-D-galacturonate mutant enzyme T113V, at pH 8.0 and 25°C Chromohalobacter salexigens
22
-
 beta-D-galacturonate mutant enzyme D246N, at pH 8.0 and 25°C Chromohalobacter salexigens
22
-
 beta-D-galacturonate mutant enzyme I88L, at pH 8.0 and 25°C Chromohalobacter salexigens
22
-
 beta-D-galacturonate mutant enzyme T113V/A172V/M175I/D236N, at pH 8.0 and 25°C Chromohalobacter salexigens
25
-
 beta-D-galacturonate mutant enzyme Y116F, at pH 8.0 and 25°C Chromohalobacter salexigens
27
-
 beta-D-galacturonate mutant enzyme A172V, at pH 8.0 and 25°C Chromohalobacter salexigens
27
-
 beta-D-galacturonate mutant enzyme L158A/N159C/I160V, at pH 8.0 and 25°C Chromohalobacter salexigens
29
-
 beta-D-galacturonate mutant enzyme T113V/M175I/D246N, at pH 8.0 and 25°C Chromohalobacter salexigens
30
-
 beta-D-galacturonate mutant enzyme T113V/A172V/D246N, at pH 8.0 and 25°C Chromohalobacter salexigens
30.8
-
 beta-D-galacturonate wild type enzyme, at pH 8.0 and 25°C Chromohalobacter salexigens
50
-
 beta-D-glucuronate mutant enzyme Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175I/D246N, at pH 8.0 and 25°C Chromohalobacter salexigens
59
-
 beta-D-glucuronate mutant enzyme N99K/L100H/K102V/P103K/I105V/Y116F/T113V/A172V/M175I/D236N, at pH 8.0 and 25°C Chromohalobacter salexigens
119
-
 beta-D-glucuronate mutant enzyme T113V/A172V/M175I, at pH 8.0 and 25°C Chromohalobacter salexigens
130
-
 beta-D-glucuronate mutant enzyme T113V/A172V/M175I/D236N, at pH 8.0 and 25°C Chromohalobacter salexigens
142
-
 beta-D-glucuronate mutant enzyme N99K/L100H/K102V/P103K/I105V, at pH 8.0 and 25°C Chromohalobacter salexigens
153
-
 beta-D-glucuronate mutant enzyme M175I, at pH 8.0 and 25°C Chromohalobacter salexigens
155
-
 beta-D-glucuronate mutant enzyme I114T, at pH 8.0 and 25°C Chromohalobacter salexigens
179
-
 beta-D-glucuronate mutant enzyme T113V/M175I/D246N, at pH 8.0 and 25°C Chromohalobacter salexigens
192
-
 beta-D-glucuronate mutant enzyme D246N, at pH 8.0 and 25°C Chromohalobacter salexigens
192
-
 beta-D-glucuronate mutant enzyme I88L, at pH 8.0 and 25°C Chromohalobacter salexigens
192
-
 beta-D-glucuronate mutant enzyme T113V, at pH 8.0 and 25°C Chromohalobacter salexigens
200
-
 beta-D-glucuronate mutant enzyme Y116F, at pH 8.0 and 25°C Chromohalobacter salexigens
214
-
 beta-D-glucuronate mutant enzyme T113V/A172V/D246N, at pH 8.0 and 25°C Chromohalobacter salexigens
219
-
 beta-D-glucuronate mutant enzyme L158A/N159C/I160V, at pH 8.0 and 25°C Chromohalobacter salexigens
225
-
 beta-D-glucuronate mutant enzyme A172V, at pH 8.0 and 25°C Chromohalobacter salexigens
263
-
 beta-D-glucuronate wild type enzyme, at pH 8.0 and 25°C Chromohalobacter salexigens
270
-
 beta-D-glucuronate mutant enzyme Q55R, at pH 8.0 and 25°C Chromohalobacter salexigens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Chromohalobacter salexigens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
41
-
 beta-D-galacturonate mutant enzyme I114T, at pH 8.0 and 25°C Chromohalobacter salexigens
48
-
 beta-D-glucuronate mutant enzyme I114T, at pH 8.0 and 25°C Chromohalobacter salexigens
77
-
 beta-D-glucuronate mutant enzyme Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175I/D246N, at pH 8.0 and 25°C Chromohalobacter salexigens
78
-
 beta-D-galacturonate mutant enzyme Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175I/D246N, at pH 8.0 and 25°C Chromohalobacter salexigens
101
-
 beta-D-glucuronate mutant enzyme Y116F, at pH 8.0 and 25°C Chromohalobacter salexigens
104
-
 beta-D-galacturonate mutant enzyme T113V/A172V/M175I, at pH 8.0 and 25°C Chromohalobacter salexigens
111
-
 beta-D-glucuronate mutant enzyme T113V/A172V/M175I, at pH 8.0 and 25°C Chromohalobacter salexigens
111
-
 beta-D-galacturonate mutant enzyme Y116F, at pH 8.0 and 25°C Chromohalobacter salexigens
112
-
 beta-D-galacturonate mutant enzyme I88L, at pH 8.0 and 25°C Chromohalobacter salexigens
115
-
 beta-D-glucuronate mutant enzyme T113V, at pH 8.0 and 25°C Chromohalobacter salexigens
116
-
 beta-D-galacturonate mutant enzyme M175I, at pH 8.0 and 25°C Chromohalobacter salexigens
125
-
 beta-D-glucuronate mutant enzyme I88L, at pH 8.0 and 25°C Chromohalobacter salexigens
125
-
 beta-D-galacturonate mutant enzyme N99K/L100H/K102V/P103K/I105V, at pH 8.0 and 25°C Chromohalobacter salexigens
125
-
 beta-D-galacturonate mutant enzyme T113V/A172V/M175I/D236N, at pH 8.0 and 25°C Chromohalobacter salexigens
126
-
 beta-D-galacturonate mutant enzyme D246N, at pH 8.0 and 25°C Chromohalobacter salexigens
126
-
 beta-D-galacturonate mutant enzyme N99K/L100H/K102V/P103K/I105V/Y116F/T113V/A172V/M175I/D236N, at pH 8.0 and 25°C Chromohalobacter salexigens
132
-
 beta-D-galacturonate mutant enzyme T113V, at pH 8.0 and 25°C Chromohalobacter salexigens
136
-
 beta-D-glucuronate mutant enzyme A172V, at pH 8.0 and 25°C Chromohalobacter salexigens
136
-
 beta-D-glucuronate mutant enzyme N99K/L100H/K102V/P103K/I105V/Y116F/T113V/A172V/M175I/D236N, at pH 8.0 and 25°C Chromohalobacter salexigens
138
-
 beta-D-galacturonate mutant enzyme T113V/A172V/D246N, at pH 8.0 and 25°C Chromohalobacter salexigens
138
-
 beta-D-glucuronate mutant enzyme T113V/A172V/M175I/D236N, at pH 8.0 and 25°C Chromohalobacter salexigens
139
-
 beta-D-glucuronate mutant enzyme T113V/A172V/D246N, at pH 8.0 and 25°C Chromohalobacter salexigens
146
-
 beta-D-glucuronate mutant enzyme D246N, at pH 8.0 and 25°C Chromohalobacter salexigens
149
-
 beta-D-glucuronate mutant enzyme M175I, at pH 8.0 and 25°C Chromohalobacter salexigens
151
-
 beta-D-galacturonate mutant enzyme A172V, at pH 8.0 and 25°C Chromohalobacter salexigens
152
-
 beta-D-galacturonate mutant enzyme Q55R, at pH 8.0 and 25°C Chromohalobacter salexigens
155
-
 beta-D-galacturonate mutant enzyme T113V/M175I/D246N, at pH 8.0 and 25°C Chromohalobacter salexigens
161
-
 beta-D-glucuronate wild type enzyme, at pH 8.0 and 25°C Chromohalobacter salexigens
161
-
 beta-D-glucuronate mutant enzyme Q55R, at pH 8.0 and 25°C Chromohalobacter salexigens
162
-
 beta-D-glucuronate mutant enzyme N99K/L100H/K102V/P103K/I105V, at pH 8.0 and 25°C Chromohalobacter salexigens
164
-
 beta-D-glucuronate mutant enzyme T113V/M175I/D246N, at pH 8.0 and 25°C Chromohalobacter salexigens
172
-
 beta-D-galacturonate wild type enzyme, at pH 8.0 and 25°C Chromohalobacter salexigens
186
-
 beta-D-glucuronate mutant enzyme L158A/N159C/I160V, at pH 8.0 and 25°C Chromohalobacter salexigens
187
-
 beta-D-galacturonate mutant enzyme L158A/N159C/I160V, at pH 8.0 and 25°C Chromohalobacter salexigens