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Literature summary for 1.1.1.203 extracted from

  • Li, Y.; Xue, Y.; Cao, Z.; Zhou, T.; Alnadari, F.
    Characterization of a uronate dehydrogenase from Thermobispora bispora for production of glucaric acid from hemicellulose substrate (2018), World J. Microbiol. Biotechnol., 34, 102 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermobispora bispora

General Stability

General Stability Organism
the enzyme is more active in PBS than in Tris-HCl buffer Thermobispora bispora

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.115
-
NAD+ at pH 7.0 and 60┬░C Thermobispora bispora
0.115
-
D-galacturonic acid at pH 7.0 and 60┬░C Thermobispora bispora
0.165
-
D-glucuronic acid from a beechwood xylan at pH 7.0 and 60┬░C Thermobispora bispora

Organism

Organism UniProt Comment Textmining
Thermobispora bispora D6Y7Y8
-
-
Thermobispora bispora DSM 43833 D6Y7Y8
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-affinity chromatography Thermobispora bispora

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
63.4
-
with D-galaceturonic acid as substrate, at pH 7.0 and 60┬░C Thermobispora bispora
95.17
-
with D-glucuronic acid from a beechwood xylan as substrate, at pH 7.0 and 60┬░C Thermobispora bispora

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galacturonic acid + NAD+
-
Thermobispora bispora D-galactaric acid + NADH + H+
-
?
D-galacturonic acid + NAD+
-
Thermobispora bispora DSM 43833 D-galactaric acid + NADH + H+
-
?
D-glucuronic acid from a beechwood xylan + NAD+ after 60 min of incubation at 50┬░C, the enzyme exhibits a conversion ratio for glucuronic acid to the glucaric acid of 84% on chemical reagent and 81.3% on hydrolysates from beechwood xylans formed by xylanase and alpha-glucuronidase Thermobispora bispora D-glucaric acid + NADH + H+
-
?
D-glucuronic acid from a beechwood xylan + NAD+ after 60 min of incubation at 50┬░C, the enzyme exhibits a conversion ratio for glucuronic acid to the glucaric acid of 84% on chemical reagent and 81.3% on hydrolysates from beechwood xylans formed by xylanase and alpha-glucuronidase Thermobispora bispora DSM 43833 D-glucaric acid + NADH + H+
-
?

Subunits

Subunits Comment Organism
? x * 31000, SDS-PAGE Thermobispora bispora
? x * 30700, calculated from amino acid sequence Thermobispora bispora

Synonyms

Synonyms Comment Organism
Udh
-
Thermobispora bispora

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
60
-
-
Thermobispora bispora

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
50 65 about 45% activity at 45┬░C, about 60% activity at 50┬░C, 100% activity at 60┬░C, about 90% activity at 65┬░C, about 20% activity at 70┬░C Thermobispora bispora

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
60 65 the purified enzyme retains over 58% of its activity after holding a pH ranging from 7.0 to 7.5 for 1 h at 60┬░C. After incubation at 65┬░C for 1 h, the activity of the enzyme is 6.8% of the original activity Thermobispora bispora

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
58.333
-
D-galacturonic acid at pH 7.0 and 60┬░C Thermobispora bispora
65.882
-
D-glucuronic acid from a beechwood xylan at pH 7.0 and 60┬░C Thermobispora bispora
74.667
-
NAD+ at pH 7.0 and 60┬░C Thermobispora bispora

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Thermobispora bispora

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 more than 40% activity between pH 6.0 and 8.5 Thermobispora bispora

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 7.5 after incubation at pH 7.0 and 7.5 for 1 h, the enzyme activity is 98.4 and 96.6%, respectively, but its stability decreases significantly below pH 7.0 or above pH 7.5 Thermobispora bispora

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Thermobispora bispora

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
400.014
-
D-glucuronic acid from a beechwood xylan at pH 7.0 and 60┬░C Thermobispora bispora
509.018
-
D-galacturonic acid at pH 7.0 and 60┬░C Thermobispora bispora
622.22
-
NAD+ at pH 7.0 and 60┬░C Thermobispora bispora