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Literature summary for 1.1.1.205 extracted from

  • Hampton, A.; Nomura, A.
    Inosine 5-phosphate dehydrogenase. Site of inhibition by guanosine 5-phosphate and of inactivation by 6-chloro- and 6-mercaptopurine ribonucleoside 5-phosphates (1967), Biochemistry, 6, 679-689.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin final concentration of 0.05% stimulates 50% Klebsiella aerogenes
glutathione level for maximum activity: 2-4 mM Klebsiella aerogenes

General Stability

General Stability Organism
activity is maintained when stored for 3 weeks at -20°C, during which it can be thawed and frozen twice and kept at 2°C for two 24-h periods Klebsiella aerogenes

Inhibitors

Inhibitors Comment Organism Structure
6-Chloropurine ribonucleoside 5'-phosphate inosine 5'-phosphate and guanosine 5'-phosphate retard inactivation Klebsiella aerogenes
6-thioinosine 5'-phosphate complete inactivation after 10 min preincubation at a concentration of 0.001 mM; in absence of glutathione Klebsiella aerogenes
glutathione above 4 mM Klebsiella aerogenes
GMP competitive with respect to IMP Klebsiella aerogenes
K+ above 0.1 M Klebsiella aerogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
6-thio-IMP pH 8.1 Klebsiella aerogenes
0.021
-
inosine 5'-phosphate 6-thioinosine 5'-phosphate, constant in pH-range 6.5-8.2, sharp decrease at higher pH Klebsiella aerogenes
1.1
-
NAD+
-
Klebsiella aerogenes

Metals/Ions

Metals/Ions Comment Organism Structure
K+ requirement of 0.1 M for maximal activity Klebsiella aerogenes
NH4+ required Klebsiella aerogenes

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.2
-
-
Klebsiella aerogenes

Storage Stability

Storage Stability Organism
2°C, 50% loss of activity after 2-3 days in dilutions with water Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-thio-IMP + NAD+ + H2O
-
Klebsiella aerogenes 6-thio-XMP + NADH + H+
-
r
6-thioinosine 5'-phosphate + NAD+ + H2O
-
Klebsiella aerogenes 6-thioxanthosine 5'-phosphate + NADH
-
?
inosine 5'-phosphate + NAD+ + H2O
-
Klebsiella aerogenes xanthosine 5'-phosphate + NADH
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
Klebsiella aerogenes

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Klebsiella aerogenes

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.13
-
GMP
-
Klebsiella aerogenes
0.14
-
6-thio-IMP
-
Klebsiella aerogenes