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Literature summary for 1.1.1.205 extracted from

  • Yamada, Y.; Natsumeda, Y.; Weber, G.
    Action of the active metabolites of tiazofurin and ribavirin on purified IMP dehydrogenase (1988), Biochemistry, 27, 2193-2196.
    View publication on PubMed

General Stability

General Stability Organism
bovine serum albumin stabilizes during dialysis Rattus norvegicus
purified enzyme very unstable Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
additional information inhibition by products and antimetabolites Rattus norvegicus
NAD+ substrate inhibition at high concentration Rattus norvegicus
NADH
-
Rattus norvegicus
ribavirin 5'-monophosphate
-
Rattus norvegicus
thiazole-4-carboxamide adenine dinucleotide
-
Rattus norvegicus
xanthosine 5'-phosphate competitive inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
IMP
-
Rattus norvegicus
0.065
-
NAD+
-
Rattus norvegicus
7.8
-
K+
-
Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
4 * 60000, rat hepatoma 3924 A cells, SDS-PAGE Rattus norvegicus
245000
-
rat hepatoma 3924 A cells, gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
hepatoma 3924A
-

Purification (Commentary)

Purification (Comment) Organism
hepatoma cells 3924 A Rattus norvegicus
homogeneitiy Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
IMP + NAD+ + H2O = XMP + NADH + H+ enzyme acts on the hydroxyl group of the hydrated derivative of the substrate, ordered bi-bi mechanism Rattus norvegicus
IMP + NAD+ + H2O = XMP + NADH + H+ IMP binds first, followed by NAD+, NADH dissociates from the ternary complex first, then XMP is released Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
Morris hepatoma 3924A cell
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
-70°C, 10 mg/ml bovine serum albumin, 3 weeks, 15% loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inosine 5'-phosphate + NAD+ + H2O
-
Rattus norvegicus xanthosine 5'-phosphate + NADH
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 60000, rat hepatoma 3924 A cells, SDS-PAGE Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0008
-
ribavirin 5'-monophosphate
-
Rattus norvegicus
0.0013
-
thiazole-4-carboxamide adenine dinucleotide
-
Rattus norvegicus
0.136
-
xanthosine 5'-phosphate
-
Rattus norvegicus
0.21
-
NADH
-
Rattus norvegicus