Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of guanine-prototrophic strain by deletion of the cystathione beta-synthase domain from the chromosomal gene of inosine 5'-monophosphate dehydrogenase. Mutant strain exhibits a substantially elevated ATP content and a slightly reduced GTP content. Activities of inosine 5'-monophosphate dehydrogenase, adenylosuccinate synthetase and GMP reductase are two- to threefold lower than in wild-type | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine 5'-phosphate + NAD+ | - |
Escherichia coli | xanthosine 5'-phosphate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IMPDH | - |
Escherichia coli |
inosine 5'-monophosphate dehydrogenase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.1 | - |
assay at | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | a guanine-prototrophic Escherichia coli strain, MP101, is constructed with the cystathionine beta-synthase subdomain deleted from the chromosomal gene for IMPDH. The ATP content is substantially elevated in mutant strain MP101 whereas the GTP content is slighty reduced. The activities of IMPDH, adenylosuccinate synthetase and GMP reductase are 2 to 3fold lower in MP101 crude extracts compared with wild-type strain. Results of a comparative analysis of the purine nucleotide pools, fluxes and turnover rates in the mutant and wild-type strains, indicate that the cystathionine beta-synthase subdomain of IMPDH plays an important role in the regulation of purine nucleotide metabolism | Escherichia coli |