Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21 (DE3) using a pGEX-KG vector | Halobacterium salinarum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | activity at 2 M NaCl is more than 4fold higher than that in the absence of salt | Halobacterium salinarum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
IMP + NAD+ + H2O | Halobacterium salinarum | rate-limiting enzyme in the de novo purine biosynthetic pathway leading to the formation of guanine ribonucleotides | XMP + NADH + H+ | - |
? | |
IMP + NAD+ + H2O | Halobacterium salinarum NRC 34002 | rate-limiting enzyme in the de novo purine biosynthetic pathway leading to the formation of guanine ribonucleotides | XMP + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halobacterium salinarum | - |
- |
- |
Halobacterium salinarum NRC 34002 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Halobacterium salinarum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.53 | - |
pH 8.0, 22°C, in absence of salt, substrate: IMP | Halobacterium salinarum |
2.41 | - |
pH 8.0, 22°C, at 2 M NaCl, substrate: IMP | Halobacterium salinarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
IMP + NAD+ + H2O | - |
Halobacterium salinarum | XMP + NADH + H+ | - |
? | |
IMP + NAD+ + H2O | rate-limiting enzyme in the de novo purine biosynthetic pathway leading to the formation of guanine ribonucleotides | Halobacterium salinarum | XMP + NADH + H+ | - |
? | |
IMP + NAD+ + H2O | - |
Halobacterium salinarum NRC 34002 | XMP + NADH + H+ | - |
? | |
IMP + NAD+ + H2O | rate-limiting enzyme in the de novo purine biosynthetic pathway leading to the formation of guanine ribonucleotides | Halobacterium salinarum NRC 34002 | XMP + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IMPDH | - |
Halobacterium salinarum |
inosine monophosphate dehydrogenase | - |
Halobacterium salinarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Halobacterium salinarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Halobacterium salinarum |
General Information | Comment | Organism |
---|---|---|
physiological function | rate-limiting enzyme in the de novo purine biosynthetic pathway leading to the formation of guanine ribonucleotides | Halobacterium salinarum |