Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Homo sapiens | |
AMP | - |
Homo sapiens | |
additional information | ATP shows no significant effect on the catalytic activity of the enzyme | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
isoform IMPDH2 in complex with GTP, hanging drop vapor diffusion method, using 25% PEG-1500 and 0.1 M buffer MIB (malonic acid, imidazole and boric acid) at pH 9.0. Isoform IMPDH2 in complex with GDP, hanging drop vapor diffusion method, using 25% PEG-1500 and 0.1 M sodium citrate at pH 5.5, 0.2 M lithium sulfate and 15% (v/v) ethanol | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D226N | the mutant enzyme cannot be allosterically inhibited in vitro neither by GTP/GDP | Homo sapiens |
K238E | the mutant enzyme cannot be allosterically inhibited in vitro neither by GTP/GDP | Homo sapiens |
L227P | the mutant enzyme cannot be allosterically inhibited in vitro neither by GTP/GDP | Homo sapiens |
N198K | the mutant enzyme cannot be allosterically inhibited in vitro neither by GTP/GDP | Homo sapiens |
R224P | the mutant enzyme cannot be allosterically inhibited in vitro neither by GTP/GDP | Homo sapiens |
R231P | the mutant enzyme cannot be allosterically inhibited in vitro neither by GTP/GDP | Homo sapiens |
Y12A | the mutation abrogates polymerization of the enzyme. Mutant enzymes remain as monodisperse tetramers in solution | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GDP | - |
Homo sapiens | |
GTP | - |
Homo sapiens | |
additional information | enzyme cytoophidia are catalytically active and resistant to GDP allosteric inhibition | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 1 mM used in assay conditions | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
IMP + NAD+ + H2O | Homo sapiens | - |
XMP + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P12268 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293T cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
IMP + NAD+ + H2O | - |
Homo sapiens | XMP + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
IMPDH2 | isoform | Homo sapiens |
inosine 5'-monophosphate dehydrogenase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens |