Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) DELTAguaB cells | Aspergillus nidulans |
expressed in Escherichia coli BL21(DE3) DELTAguaB cells | Penicillium brevicompactum |
Protein Variants | Comment | Organism |
---|---|---|
A267S | the mutant enzyme is inhibited by mycophenolic acid | Aspergillus nidulans |
S267A | the mutant enzyme of isoform IMPDHA is inhibited by mycophenolic acid | Penicillium brevicompactum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not inhibited by mycophenolic acid | Aspergillus nidulans | |
additional information | the enzyme is not inhibited by mycophenolic acid | Penicillium brevicompactum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
IMP | wild type enzyme, at pH 8.0 and 25°C | Aspergillus nidulans | |
0.015 | - |
IMP | mutant enzyme A267S, at pH 8.0 and 25°C | Aspergillus nidulans | |
0.079 | - |
IMP | mutant enzyme S267A of isoform IMPDHA, at pH 8.0 and 25°C | Penicillium brevicompactum | |
0.093 | - |
IMP | wild type enzyme isoform IMPDHA, at pH 8.0 and 25°C | Penicillium brevicompactum | |
0.12 | - |
NAD+ | wild type enzyme, at pH 8.0 and 25°C | Aspergillus nidulans | |
0.19 | - |
NAD+ | mutant enzyme A267S, at pH 8.0 and 25°C | Aspergillus nidulans | |
0.33 | - |
NAD+ | mutant enzyme S267A of isoform IMPDHA, at pH 8.0 and 25°C | Penicillium brevicompactum | |
0.4 | - |
NAD+ | wild type enzyme isoform IMPDHA, at pH 8.0 and 25°C | Penicillium brevicompactum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
IMP + NAD+ + H2O | Aspergillus nidulans | - |
XMP + NADH + H+ | - |
? | |
IMP + NAD+ + H2O | Penicillium brevicompactum | - |
XMP + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus nidulans | - |
- |
- |
Penicillium brevicompactum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
IMP + NAD+ + H2O | - |
Aspergillus nidulans | XMP + NADH + H+ | - |
? | |
IMP + NAD+ + H2O | - |
Penicillium brevicompactum | XMP + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IMPDH | - |
Aspergillus nidulans |
IMPDHA | isoform | Aspergillus nidulans |
IMPDHA | isoform | Penicillium brevicompactum |
inosine 5'-monophosphate dehydrogenase | - |
Aspergillus nidulans |
inosine 5'-monophosphate dehydrogenase | - |
Penicillium brevicompactum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
NAD+ | mutant enzyme S267A of isoform IMPDHA, at pH 8.0 and 25°C | Penicillium brevicompactum | |
1.6 | - |
IMP | mutant enzyme S267A of isoform IMPDHA, at pH 8.0 and 25°C | Penicillium brevicompactum | |
1.9 | - |
NAD+ | wild type enzyme, at pH 8.0 and 25°C | Aspergillus nidulans | |
1.9 | - |
IMP | wild type enzyme, at pH 8.0 and 25°C | Aspergillus nidulans | |
1.9 | - |
NAD+ | mutant enzyme A267S, at pH 8.0 and 25°C | Aspergillus nidulans | |
1.9 | - |
IMP | mutant enzyme A267S, at pH 8.0 and 25°C | Aspergillus nidulans | |
2.7 | - |
NAD+ | wild type enzyme isoform IMPDHA, at pH 8.0 and 25°C | Penicillium brevicompactum | |
2.7 | - |
IMP | wild type enzyme isoform IMPDHA, at pH 8.0 and 25°C | Penicillium brevicompactum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Aspergillus nidulans | |
NAD+ | - |
Penicillium brevicompactum |