Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-thiabicyclo(3,2,1)octane-3-one | complete inhibition at 4 mM | Hyoscyamus niger | |
Ca2+ | 0-15% inactivation at 1 mM | Hyoscyamus niger | |
Cd2+ | 26-43% inactivation at 1 mM | Hyoscyamus niger | |
Co2+ | 0-15% inactivation at 1 mM | Hyoscyamus niger | |
Cu2+ | 99% inactivation at 1 mM | Hyoscyamus niger | |
diethyl dicarbonate | complete inhibition at 0.1 mM | Hyoscyamus niger | |
Fe2+ | 0-15% inactivation at 1 mM | Hyoscyamus niger | |
Fe3+ | 0-15% inactivation at 1 mM | Hyoscyamus niger | |
Hg2+ | 99% inactivation at 1 mM | Hyoscyamus niger | |
Li+ | 0-15% inactivation at 1 mM | Hyoscyamus niger | |
Mg2+ | 0-15% inactivation at 1 mM | Hyoscyamus niger | |
Mn2+ | 0-15% inactivation at 1 mM | Hyoscyamus niger | |
additional information | not inhibited by EDTA and iodoacetamide | Hyoscyamus niger | |
Ni2+ | 0-15% inactivation at 1 mM | Hyoscyamus niger | |
p-chloromercuribenzoate | strong inhibition at 0.1 and 1.0 mM | Hyoscyamus niger | |
Zn2+ | 0-15% inactivation at 1 mM | Hyoscyamus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
4-methylcyclohexanone | - |
Hyoscyamus niger | |
0.003 | - |
4-ethylcyclohexanone | - |
Hyoscyamus niger | |
0.003 | - |
8-thiabicyclo(3,2,1)octane-3-one | - |
Hyoscyamus niger | |
0.004 | - |
3-methylcyclohexanone | - |
Hyoscyamus niger | |
0.011 | - |
NADPH | - |
Hyoscyamus niger | |
0.042 | - |
NADP+ | - |
Hyoscyamus niger | |
0.231 | - |
N-Methyl-4-piperidone | - |
Hyoscyamus niger | |
1.01 | - |
tropinone | - |
Hyoscyamus niger | |
1.81 | - |
3-quinuclidinone | - |
Hyoscyamus niger | |
2.6 | - |
Tropine | - |
Hyoscyamus niger | |
10 | - |
NADH | - |
Hyoscyamus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
SDS-PAGE | Hyoscyamus niger |
115000 | - |
gel filtration | Hyoscyamus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Atropa acuminata | - |
- |
- |
Atropa belladonna | - |
- |
- |
Datura stramonium | - |
- |
- |
Duboisia hopwoodii | - |
- |
- |
Duboisia leichhardtii | - |
- |
- |
Hyoscyamus albus | - |
- |
- |
Hyoscyamus bohemicus | - |
- |
- |
Hyoscyamus canariensis | - |
- |
- |
Hyoscyamus muticus | - |
- |
- |
Hyoscyamus niger | - |
and strain Hn11 | - |
Hyoscyamus niger Hn | - |
and strain Hn11 | - |
Hyoscyamus pusillus | - |
- |
- |
no activity in Brassica campestris | - |
- |
- |
no activity in Browallia americana | - |
- |
- |
no activity in Calystegia sepium | - |
- |
- |
no activity in Nicotiana tabacum | - |
- |
- |
no activity in Physalis alkekengi | - |
- |
- |
Physalis edulis | - |
- |
- |
Physalis philadelphica | - |
- |
- |
Physochlaina orientalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Hyoscyamus niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Datura stramonium | - |
root | - |
Datura stramonium | - |
root | - |
Atropa belladonna | - |
root | - |
Hyoscyamus niger | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.6 | - |
tropinone | Hyoscyamus albus |
1.2 | - |
tropinone | Hyoscyamus bohemicus |
1.2 | - |
tropinone | Atropa acuminata |
3 | - |
tropinone | Physochlaina orientalis |
7.5 | - |
tropinone, strain Hn | Hyoscyamus niger |
7.5 | - |
tropinone | Atropa belladonna |
7.5 | - |
tropinone | Hyoscyamus canariensis |
10.2 | - |
tropinone | Hyoscyamus pusillus |
10.8 | - |
tropinone | Hyoscyamus muticus |
15 | - |
tropinone, strain Hn11 | Hyoscyamus niger |
30 | - |
tropinone | Duboisia leichhardtii |
30 | - |
tropinone | Duboisia hopwoodii |
45 | - |
tropinone | Datura stramonium |
54 | - |
tropinone | Physalis edulis |
66 | - |
tropinone | Physalis philadelphica |
Storage Stability | Organism |
---|---|
4°C, NADPH, NADP+ and glycerol enhance stability | Hyoscyamus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylcyclohexanone + NADPH | - |
Datura stramonium | 3-methylcyclohexanol + NADP+ | - |
? | |
3-methylcyclohexanone + NADPH | - |
Hyoscyamus niger | 3-methylcyclohexanol + NADP+ | - |
? | |
3-methylcyclohexanone + NADPH | - |
Hyoscyamus niger Hn | 3-methylcyclohexanol + NADP+ | - |
? | |
3-quinuclidinone + NADPH + H+ | - |
Datura stramonium | quinuclidin-3-ol + NADP+ | - |
? | |
3-quinuclidinone + NADPH + H+ | - |
Hyoscyamus niger | quinuclidin-3-ol + NADP+ | - |
? | |
4-ethylcyclohexanone + NADPH | - |
Datura stramonium | 4-ethylcyclohexanol + NADP+ | - |
? | |
4-ethylcyclohexanone + NADPH | - |
Hyoscyamus niger | 4-ethylcyclohexanol + NADP+ | - |
? | |
4-ethylcyclohexanone + NADPH | - |
Hyoscyamus niger Hn | 4-ethylcyclohexanol + NADP+ | - |
? | |
4-methylcyclohexanone + NADPH | - |
Datura stramonium | 4-methylcyclohexanol + NADP+ | - |
? | |
4-methylcyclohexanone + NADPH | - |
Hyoscyamus niger | 4-methylcyclohexanol + NADP+ | - |
? | |
4-methylcyclohexanone + NADPH | - |
Hyoscyamus niger Hn | 4-methylcyclohexanol + NADP+ | - |
? | |
8-thiabicyclo(3,2,1)octane-3-one + NADPH | - |
Hyoscyamus niger | 8-thiabicyclo(3,2,1)octane-3-ol + NADP+ | - |
? | |
N-methyl-4-piperidone + NADPH | - |
Datura stramonium | 1-methylpiperidin-4-ol + NADP+ | - |
? | |
N-methyl-4-piperidone + NADPH | - |
Hyoscyamus niger | 1-methylpiperidin-4-ol + NADP+ | - |
? | |
N-methyl-4-piperidone + NADPH | - |
Hyoscyamus niger Hn | 1-methylpiperidin-4-ol + NADP+ | - |
? | |
tropine + NADP+ | - |
Datura stramonium | tropinone + NADPH | - |
r | |
tropine + NADP+ | - |
Hyoscyamus niger | tropinone + NADPH | - |
r | |
tropine + NADP+ | - |
Hyoscyamus niger Hn | tropinone + NADPH | - |
r | |
tropinone + NADH + H+ | - |
Hyoscyamus niger | tropine + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 30000, gel filtration | Hyoscyamus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.1 | - |
tropinone reduction, narrow pH-range | Hyoscyamus niger |
7.6 | - |
tropine oxidation | Hyoscyamus niger |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | lower affinity than with NADPH | Hyoscyamus niger | |
NADPH | - |
Datura stramonium | |
NADPH | - |
Hyoscyamus niger |