Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM inhibits complex formation of ALR 2 with alpha-crystallin | Bos taurus | |
Mg2+ | 10 mM inhibits complex formation of ALR 2 with alpha-crystallin, induces ALR 2 aggregation and precipitation | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
isozyme ALR 2, gel filtration | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
2 enzymes AR I (ALR 1) and AR II (ALR 2) | - |
Purification (Comment) | Organism |
---|---|
ALR 2 | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lens | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.2 | - |
NADPH, purified enzyme | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-glyceraldehyde + NAD(P)H | - |
Bos taurus | glycerol + NAD(P)+ | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
thermal aggregation and precipitation is prevented by specific, thermal stress induced complexing of alpha-crystallin, stable at least for 2 h at 55°C and for hours at room temperature, complexing can be hindered by Tris | Bos taurus |