Identification of histidine residue in the active site of fetal lamb 3beta,20alpha-hydroxysteroid oxidoreductase

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

5alpha-dihydrotestosterone 17-bromoacetate

competitive inhibition of 3beta- and 20alpha-reductase activity, time dependent and simultaneous, complete inactivation

Ovis aries

Reaction

Commentary

Organism

Reaction ID

5alpha-androstan-3beta,17beta-diol + NADP+ = 17beta-hydroxy-5alpha-androstan-3-one + NADPH + H+

3beta and 20alpha substrates share the same active site

Ovis aries

Cofactor

Commentary

Organism

Structure

NADP+

-

Ovis aries

NADPH

-

Ovis aries

Cofactor

Commentary

Organism

Structure

NADP+

-

Ovis aries

NADPH

-

Ovis aries

Inhibitors

Commentary

Organism

Structure

5alpha-dihydrotestosterone 17-bromoacetate

competitive inhibition of 3beta- and 20alpha-reductase activity, time dependent and simultaneous, complete inactivation

Ovis aries

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

657329

Clementi

Luteal 3beta-hydroxysteroid de ...

Rattus norvegicus

Reprod. Biol. Endocrinol.

2

22

2004

-

-

-

-

-

-

-

-

1

-

-

1

-

1

-

-

-

-

-

1

-

-

1

-

-

1

-

-

-

1

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

1

-

-

1

-

-

-

-

-

1

-

-

1

-

1

-

-

-

1

-

-

-

-

-

-

-

-

-

286268

Chen

-

Identification of histidine re ...

Ovis aries

Sheng Wu Hua Xue Yu Sheng Wu Wu Li Jin Zhan

18

113-117

1991

-

-

-

-

-

-

1

-

1

-

-

-

-

1

-

-

-

1

-

1

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

2

-

-

-

-

1

-

-

1

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

286267

Chen

Fetal lamb 3beta,20alpha-hydro ...

Ovis aries

Biochemistry

28

8856-8863

1989

-

-

-

-

-

-

-

1

1

-

2

-

-

1

-

-

1

1

-

1

1

1

2

1

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

2

-

-

-

-

-

-

1

1

-

2

-

-

-

-

1

-

1

1

1

2

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

286266

Chen

Isolation of 3beta,20alpha-hyd ...

Ovis aries

Steroids

49

477-496

1987

-

-

-

-

-

2

3

2

1

-

2

2

-

1

-

-

1

1

-

1

1

-

3

1

-

-

-

-

-

-

-

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2

-

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-

2

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2

-

3

-

2

1

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2

2

-

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-

1

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1

1

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3

1

-

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-

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-

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-

-

-

-

-

-

-

-

286265

Sharaf

Dual activity at an enzyme act ...

Bos taurus

Biochemistry

21

4615-4620

1982

-

-

-

-

-

1

3

2

1

-

1

2

-

1

-

-

1

1

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1

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1

3

1

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1

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1

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2

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2

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1

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1

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1

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1

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1

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1

3

1

1

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1

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