Cloned (Comment) | Organism |
---|---|
expression of recombinant wild type and mutants of AKR1C9 | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
F118A | largest changes in kcat/Km | Rattus norvegicus |
F129A | largest changes in kcat/Km, F129A alters the stereochemical preference from oxidizing predominantly the S,S-stereoisomer to oxidizing predominantly the R,R-stereoisomer of benzo[g]chrysene-11,12-dihydrodiol | Rattus norvegicus |
L54A | mutant shows intermediate changes in kcat/Km versus wild type | Rattus norvegicus |
N306A | mutant shows intermediate changes in kcat/Km versus wild type | Rattus norvegicus |
T226A | smallest change in kcat/Km is observed when alanine is used to substitute hydrophilic residues | Rattus norvegicus |
T24A | smallest change in kcat/Km is observed when alanine is used to substitute hydrophilic residues | Rattus norvegicus |
W227A | largest changes in kcat/Km | Rattus norvegicus |
Y310A | mutant shows intermediate changes in kcat/Km versus wild type | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DMSO | 33% inhibition | Rattus norvegicus | |
naphthalene-1,2-dione | naphthalene-1,2-dione leads to the time and concentration dependent irreversible inactivation of AKR1C9 via alkylation | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
benzo[a]pyrene-7,8-dihydrodiol | kcat/Km of 39 min/mM | Rattus norvegicus | |
additional information | - |
(11S,12S)-11,12-dihydrobenzo[g]chrysene-11,12-diol | racemic benzo[g]chrysene-11,12-dihydrodiol derived from the fjord-region parent hydrocarbon benzo[g]chrysene is oxidized with a kcat/Km of 1520 min/mM, which is more than 100 times greater than that observed with benzo[a]pyrene-7,8-dihydrodiol | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P23457 | - |
- |
Purification (Comment) | Organism |
---|---|
purification of recombinant wild type and mutants of AKR1C9 | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.6 | - |
wild type homogeneous recombinant AKR1C9, at 25°C | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(11S,12S)-11,12-dihydrobenzo[g]chrysene-11,12-diol + NADP+ | enzyme is stereoselective for benzo[g]chrysene-11S,12S-dihydrodiol | Rattus norvegicus | ? + NADPH + H+ | - |
? | |
benzo[c]phenanthrene-3,4-dihydrodiol + NADP+ | enzyme metabolizes both stereoisomers of benzo[c]phenanthrene-3,4-dihydrodiol | Rattus norvegicus | ? + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AKR1C9 | - |
Rattus norvegicus |
dihydrodiol dehydrogenase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Rattus norvegicus |