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Literature summary for 1.1.1.213 extracted from

  • Mythen, S.; Devendran, S.; Méndez-García, C.; Cann, I.; Ridlon, J.
    Targeted synthesis and characterization of a gene cluster encoding NAD(P)H-dependent 3alpha-, 3beta-, and 12alpha-hydroxysteroid dehydrogenases from Eggerthella CAG 298, a gut metagenomic sequence (2018), Appl. Environ. Microbiol., 84, e02475-17 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
heterologous expression in Escherichia coli Eggerthella sp. CAG:298

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.155
-
deoxycholic acid coenzyme: NADP+, pH 10, temperature not specified in the publication Eggerthella sp. CAG:298
0.179
-
3-oxo-deoxycholic acid coenzyme: NADPH, pH 7.0, temperature not specified in the publication Eggerthella sp. CAG:298

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26355
-
calculation from sequence Eggerthella sp. CAG:298

Organism

Organism UniProt Comment Textmining
Eggerthella sp. CAG:298 R7B6R4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Eggerthella sp. CAG:298

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-oxo-cholic acid + NAD(P)H + H+ 84.5% of the activity compared to the substrate 3-oxodeoxycholic acid Eggerthella sp. CAG:298 cholic acid + NAD(P)+
-
r
3-oxo-deoxycholic acid + NAD(P)H + H+
-
Eggerthella sp. CAG:298 deoxycholic acid + NAD(P)+
-
r
chenodeoxycholic acid + NAD(P)+ 19.8% of the activity compared to the substrate 3-oxodeoxycholic acid Eggerthella sp. CAG:298 3-oxo-chenodeoxycholic acid + NAD(P)H + H+
-
r
cholic acid + NAD(P)+ 65.5% of the activity compared to the substrate 3-oxodeoxycholic acid Eggerthella sp. CAG:298 3-oxo-cholic acid + NAD(P)H + H+
-
r
deoxycholic acid + NAD(P)+ 71.7% of the activity compared to the substrate 3-oxodeoxycholic acid Eggerthella sp. CAG:298 3-oxo-deoxycholic acid + NAD(P)H + H+
-
r
glycochenodeoxycholic acid + NAD(P)+ 50.7% of the activity compared to the substrate 3-oxodeoxycholic acid Eggerthella sp. CAG:298 ? + NAD(P)H + H+
-
r
glycocholic acid + NAD(P)+ 61.2% of the activity compared to the substrate 3-oxodeoxycholic acid Eggerthella sp. CAG:298 ? + NAD(P)H + H+
-
r
glycodeoxycholic acid + NAD(P)+ 57.8% of the activity compared to the substrate 3-oxodeoxycholic acid Eggerthella sp. CAG:298 ? + NAD(P)H + H+
-
r
additional information no activity with isodeoxycholic acid Eggerthella sp. CAG:298 ?
-
-
taurochenodeoxycholic acid + NAD(P)+ 53.3% of the activity compared to the substrate 3-oxodeoxycholic acid Eggerthella sp. CAG:298 ? + NAD(P)H + H+
-
r
taurocholic acid + NAD(P)+ 63.9% of the activity compared to the substrate 3-oxodeoxycholic acid Eggerthella sp. CAG:298 ? + NAD(P)H + H+
-
r
taurodeoxychloic acid + NAD(P)+ 59.2% of the activity compared to the substrate 3-oxodeoxycholic acid Eggerthella sp. CAG:298 ? + NAD(P)H + H+
-
r

Subunits

Subunits Comment Organism
? x * 26355, calculation from sequence Eggerthella sp. CAG:298

Synonyms

Synonyms Comment Organism
3alpha-HSDH
-
Eggerthella sp. CAG:298
CDD59474
-
Eggerthella sp. CAG:298

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
54.5
-
3-oxo-deoxycholic acid coenzyme: NADPH, pH 7.0, temperature not specified in the publication Eggerthella sp. CAG:298
55.9
-
deoxycholic acid coenzyme: NADP+, pH 10, temperature not specified in the publication Eggerthella sp. CAG:298

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
reductive reaction Eggerthella sp. CAG:298
10
-
oxidative reaction Eggerthella sp. CAG:298

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Eggerthella sp. CAG:298
NADPH
-
Eggerthella sp. CAG:298

General Information

General Information Comment Organism
physiological function enzyme in an iso-bile acid pathway Eggerthella sp. CAG:298

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.015
-
3-oxodeoxycholic acid coenzyme: NADPH, pH 6.5, temperature not specified in the publication Eggerthella sp. CAG:298
0.078
-
isodeoxycholic acid coenzyme: NADP+, pH 9.5, temperature not specified in the publication Eggerthella sp. CAG:298
71.6
-
deoxycholic acid coenzyme: NADP+, pH 7.0, temperature not specified in the publication Eggerthella sp. CAG:298
304.5
-
3-oxo-deoxycholic acid coenzyme: NADPH, pH 7.0, temperature not specified in the publication Eggerthella sp. CAG:298
360.6
-
deoxycholic acid coenzyme: NADP+, pH 10, temperature not specified in the publication Eggerthella sp. CAG:298
581.6
-
12-oxo-deoxycholic acid coenzyme: NADPH, pH 7.0, temperature not specified in the publication Eggerthella sp. CAG:298