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Literature summary for 1.1.1.214 extracted from

  • Cheng, P.; Wang, J.; Wu, Y.; Jiang, X.; Pei, X.; Su, W.
    Recombinant expression and molecular insights into the catalytic mechanism of an NADPH-dependent conjugated polyketone reductase for the asymmetric synthesis of (R)-pantolactone (2019), Enzyme Microb. Technol., 126, 77-85 .
    View publication on PubMed
Search for more literature for 1.1.1.214

Application

Application Comment Organism
synthesis in a continuous feeding reaction, 200 mM ketopantolactone is reduced to (R)-pantolactone with 98% conversion and 99% enantiomeric excess within 2.0 h Candida dubliniensis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Candida dubliniensis

Protein Variants

Protein Variants Comment Organism
H128A about 20% of wild-type activity Candida dubliniensis
W30A about 54% of wild-type activity Candida dubliniensis
Y66A complete loss of activity Candida dubliniensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.484
-
 2-dehydropantolactone wild-type, pH 7, 30°C Candida dubliniensis
3.5
-
 2-dehydropantolactone mutant T30A, pH 7, 30°C Candida dubliniensis
20.4
-
 2-dehydropantolactone mutant H128A, pH 7, 30°C Candida dubliniensis

Metals/Ions

Metals/Ions Comment Organism Structure
Ag+ 1 mM, no residual activity Candida dubliniensis
Co2+ 1 mM, 75.3% residual activity Candida dubliniensis
Cu2+ 1 mM, 29.7% residual activity Candida dubliniensis
EDTA 1 mM, 85.7% residual activity Candida dubliniensis
Fe2+ 1 mM, 66.8% residual activity Candida dubliniensis
Mg2+ 1 mM, 56.8% residual activity Candida dubliniensis
Mg2+ 1 mM, 77.6% residual activity Candida dubliniensis
Ni2+ 1 mM, 88.5% residual activity Candida dubliniensis
Pb2+ 1 mM, no residual activity Candida dubliniensis
Zn2+ 1 mM, 10.8% residual activity Candida dubliniensis

Organism

Organism UniProt Comment Textmining
Candida dubliniensis
-
-
-
Candida dubliniensis CD36
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Candida dubliniensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydropantolactone + NADPH + H+
-
 Candida dubliniensis (R)-pantolactone + NADP+
-
 ?
2-dehydropantolactone + NADPH + H+
-
 Candida dubliniensis CD36 (R)-pantolactone + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
CPR
-
 Candida dubliniensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6
-
 2-dehydropantolactone mutant H128A, pH 7, 30°C Candida dubliniensis
15.5
-
 2-dehydropantolactone mutant T30A, pH 7, 30°C Candida dubliniensis
59
-
 2-dehydropantolactone wild-type, pH 7, 30°C Candida dubliniensis

General Information

General Information Comment Organism
metabolism residue Tyr66 functions as a proton donor following hydrogen transfer from NADPH. Thr30 and His128 are critical residues to bind and orient substrate 2-dehydropantolactone Candida dubliniensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.295
-
 2-dehydropantolactone mutant H128A, pH 7, 30°C Candida dubliniensis
4.43
-
 2-dehydropantolactone mutant T30A, pH 7, 30°C Candida dubliniensis
122
-
 2-dehydropantolactone wild-type, pH 7, 30°C Candida dubliniensis