Cloned (Comment) | Organism |
---|---|
gene ga2dh | Gluconobacter japonicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a mutant strain of Gluconobacter japonicus NBRC3271 is engineered to produce 2-dehydro-D-gluconate efficiently from a mixture of D-glucose and D-gluconate | Gluconobacter japonicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Gluconobacter japonicus | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-gluconate + NADP+ | Gluconobacter japonicus | - |
2-dehydro-D-gluconate + NADPH + H+ | - |
? | |
D-gluconate + NADP+ | Gluconobacter japonicus NBRC3271 | - |
2-dehydro-D-gluconate + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter japonicus | - |
- |
- |
Gluconobacter japonicus NBRC3271 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-gluconate + NADP+ | - |
Gluconobacter japonicus | 2-dehydro-D-gluconate + NADPH + H+ | - |
? | |
D-gluconate + NADP+ | - |
Gluconobacter japonicus NBRC3271 | 2-dehydro-D-gluconate + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-ketogluconate dehydrogenase | - |
Gluconobacter japonicus |
GADH | - |
Gluconobacter japonicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Gluconobacter japonicus |
General Information | Comment | Organism |
---|---|---|
physiological function | Gluconobacter oxydans NBRC3293 produces 2,5-dioxo-D-gluconate from D-glucose via D-gluconate and 2-keto-D-gluconate, with accumulation of the product in the culture medium, the efficiency of 2,5-diketo-D-gluconate production is unsatisfactory because there is a large amount of residual D-gluconate at the end of the biotransformation process. Heterologous overexpression of the kgdSLC genes in a mutant strain of Gluconobacter japonicus NBRC3271 engineered to produce 2-dehydro-D-gluconate efficiently from a mixture of D-glucose and D-gluconate, results in a mutant strain that consumes almost all of the starting materials (D-glucose and D-gluconate) to produce 2,5-dioxo-D-gluconate quantitatively as a seemingly unique metabolite | Gluconobacter japonicus |