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Literature summary for 1.1.1.215 extracted from

  • Kataoka, N.; Matsutani, M.; Yakushi, T.; Matsushita, K.
    Efficient production of 2,5-diketo-D-gluconate via heterologous expression of 2-ketogluconate dehydrogenase in Gluconobacter japonicus (2015), Appl. Environ. Microbiol., 81, 3552-3560.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene ga2dh Gluconobacter japonicus

Protein Variants

Protein Variants Comment Organism
additional information a mutant strain of Gluconobacter japonicus NBRC3271 is engineered to produce 2-dehydro-D-gluconate efficiently from a mixture of D-glucose and D-gluconate Gluconobacter japonicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Gluconobacter japonicus 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-gluconate + NADP+ Gluconobacter japonicus
-
2-dehydro-D-gluconate + NADPH + H+
-
?
D-gluconate + NADP+ Gluconobacter japonicus NBRC3271
-
2-dehydro-D-gluconate + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Gluconobacter japonicus
-
-
-
Gluconobacter japonicus NBRC3271
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-gluconate + NADP+
-
Gluconobacter japonicus 2-dehydro-D-gluconate + NADPH + H+
-
?
D-gluconate + NADP+
-
Gluconobacter japonicus NBRC3271 2-dehydro-D-gluconate + NADPH + H+
-
?

Synonyms

Synonyms Comment Organism
2-ketogluconate dehydrogenase
-
Gluconobacter japonicus
GADH
-
Gluconobacter japonicus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Gluconobacter japonicus

General Information

General Information Comment Organism
physiological function Gluconobacter oxydans NBRC3293 produces 2,5-dioxo-D-gluconate from D-glucose via D-gluconate and 2-keto-D-gluconate, with accumulation of the product in the culture medium, the efficiency of 2,5-diketo-D-gluconate production is unsatisfactory because there is a large amount of residual D-gluconate at the end of the biotransformation process. Heterologous overexpression of the kgdSLC genes in a mutant strain of Gluconobacter japonicus NBRC3271 engineered to produce 2-dehydro-D-gluconate efficiently from a mixture of D-glucose and D-gluconate, results in a mutant strain that consumes almost all of the starting materials (D-glucose and D-gluconate) to produce 2,5-dioxo-D-gluconate quantitatively as a seemingly unique metabolite Gluconobacter japonicus